Antibody responses to SARS-CoV-2 in patients with COVID-19

Long, Quan; Liu, Bai Zhong; Deng, Hai; Wu, Gui Cheng; Deng, Kun; Chen, Yao Kai; Liao, Pu; Qiu, Jing Fu; Lin, Yong; Cai, Xue; Wang, De Qiang; Hu, Yuan; Ren, Ji Hua; Tang, Ni; Xu, Yingkun; Yu, Li; Zhan, Ming; Gong, Fang; Zhang, Xiao Li; Tian, Wen; Hu, Li; Zhang, Xian Xiang; Xiang, Jiang; Du, Hong Xin; Liu, Hua Wen; Lang, Chunhui; Luo, Xiao He; Wu, Shourong; Cui, Xiao; Zheng, Zhou; Zhu, Man; Wang, Jing; Xue, Cheng Jun; Li, Xiao Feng; Wang, Li; Li, Zhi Jie; Wang, Kun; Niu, Chang; Yang, Qing; Tang, Xiao; Zhang, Yong; Liu, Xia Mao; Li, Jin Jing; Zhang, De Chun; Zhang, Fan; Liu, Ping; Yuan, Jun; Li, Qin; Hu, Jie; Chen, Juan; Huang, Ailong

doi:10.1038/s41591-020-0897-1

Cited by 2,716 publications

(3,001 citation statements)

References 7 publications

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“…The time required for the immune system to produce detectable levels of immunoglobulins typically ranges from 5 to 10 days, but this may take longer in the case of SARS-CoV-2 [7,12,13]. The Immunochromatographic Lateral Flow Assay (Wondfo SARS-CoV-2 Antibody Test, China) [14] used in this study detects IgG and IgM combined antibodies in blood, plasma or serum samples.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity and specificity of a rapid test for assessment of exposure to SARS-CoV-2 in a community-based setting in Brazil

Pellanda

2020

Preprint

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Background: While the recommended laboratory diagnosis of COVID-19 is a molecular based assay, population-based studies to determine the prevalence of COVID-19 usually use serological assays. Objective: To evaluate the sensitivity and specificity of a rapid diagnostic test for COVID-19 compared to quantitative reverse transcription polymerase chain reaction (qRT-PCR). Methods: We evaluated the sensitivity using a panel of finger prick blood samples from participants >18 years of age that had been tested for COVID-19 by qRT-PCR. For assessing specificity, we used serum samples from the 1982 Pelotas (Brazil) Birth Cohort participants collected in 2012 with no exposure to SARS-CoV-2. Results: The sensitivity of the test was 77.1% (95% CI 66.6 -85.6), based upon 83 subjects who had tested positive for qRT-PCR at least 10 days before the rapid diagnostic test (RDT). Based upon 100 sera samples, specificity was 98.0% (95% CI 92.9 -99.8). There was substantial agreement (Kappa score 0.76) between the qRT-PCR results and the RDT. Interpretation. The validation results are well in line with previous assessments of the test, and confirm that it is sufficiently precise for epidemiological studies aimed at monitoring levels and trends of the COVID-19 pandemic.

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Section: Introductionmentioning

confidence: 99%

Sensitivity and specificity of a rapid test for assessment of exposure to SARS-CoV-2 in a community-based setting in Brazil

Pellanda

2020

Preprint

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“…Highly accurate detection of antibodies against SARS-CoV-2 is crucial to combating the pandemic by aiding diagnosis and assessing infection timing, prevalence, duration of antibody response, and potential immunity. Thus far, a variety of antibody tests have been developed using lateral flow rapid tests, enzyme-linked immunosorbent assay (ELISA) and chemiluminescence (CLIA) platforms, including ones authorized by the U.S. Food and Drug Administration (FDA) for emergency use 2,[13][14][15][16][17][18][19][20][21] . Despite the progress, few assays differentiate antibody subtypes to glean infection timing with > 99.5 % specificity for both IgG and IgM 22,23 .…”

mentioning

confidence: 99%

High-Accuracy Multiplexed SARS-CoV-2 Antibody Assay with Avidity and Saliva Capability on a Nano-Plasmonic Platform

Liu¹,

Hsiung²,

Zhao³

et al. 2020

Preprint

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The outbreak and rapid spread of SARS-CoV-2 virus has led to a dire global pandemic with millions of people infected and ~ 400,000 deaths thus far. Highly accurate detection of antibodies for COVID-19 is an indispensable part of the effort to combat the pandemic 1,2 . Here we developed two-plex antibody detection against SARS-CoV-2 spike proteins 3 (the S1 subunit and receptor binding domain RBD) in human serum and saliva on a near-infrared nanoplasmonic gold (pGOLD) platform 4-8 . By testing nearly 600 serum samples, pGOLD COVID-19 assay achieved ~ 99.78 % specificity for detecting both IgG and IgM with 100 % sensitivity in sera collected > 14 days post disease symptom onset, with zero cross-reactivity to other diseases. Two-plex correlation analysis revealed higher binding of serum IgM to RBD than to S1. IgG antibody avidity toward multiple antigens were measured, shedding light on antibody maturation in COVID-19 patients and affording a powerful tool for differentiating recent from remote infections and identifying re-infection by SARS-CoV-2. Just as important, due to high analytical sensitivity, the pGOLD COVID-19 assay detected minute amounts of antibodies in human saliva, offering the first non-invasive detection of SARS-CoV-2 antibodies.

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“…Thus, NP RT-PCR is the current recommended investigation [31]. Conflicting findings, varying accessibility, and rapid innovation make it challenging to recommend a single ideal serological test [32]. However, the use of available serological testing as an adjunct with either NP or OP RT-PCR has been shown to yield combined sensitivities of >98% [33,34].…”

Section: Preoperative Testingmentioning

confidence: 99%

Addressing Coronavirus Disease 2019 in Spine Surgery: A Rapid National Consensus Using the Delphi Method via Teleconference

et al. 2020

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The magnitude and potential duration of the current coronavirus disease 2019 pandemic is something that most doctors currently in practice have yet to experience. While considerable information regarding COVID-19 is being published every day, it is challenging to filter out the most relevant or appropriate information for our individual practice. The Spine Society of Singapore convened via a teleconference on April 24, 2020 to collaborate on a national level and share collective wisdom in order to tackle the ongoing crisis. In the teleconference, 13 spine surgeons from across various hospitals in Singapore constituted the panel of experts.The following topics were discussed: repurposing of surgeons, continuity of spine services, introduction of telemedicine, triaging of spinal surgeries, preoperative testing, new challenges in performing spine surgery, and preparing for the post-pandemic era. While some issues required only the sharing of best practices, the Delphi panel method was adopted to form a consensus on others. Existing spine specific triage guidelines were debated and a locally accepted set of guidelines was established. Although preoperative testing is currently not performed routinely, the panel voted in favor of its implementation because they concluded that it is vital to protect themselves, their colleagues, and their patients. Solutions to operating room specific concerns were also discussed. This article reflects the opinions and insights shared during this meeting and reviews the evidence relevant to the issues that were raised. The rapid consensus reached during the teleconference has enabled us to be concerted, and thus stronger, in our national efforts to provide the best standard of care via our spine services in these challenging times. We believe that this article will provide some guidance for addressing COVID-19 in spine surgery and encourage other national/regional societies to conduct similar discussions that would help their navigation of this pandemic.

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Antibody responses to SARS-CoV-2 in patients with COVID-19

Cited by 2,716 publications

References 7 publications

Sensitivity and specificity of a rapid test for assessment of exposure to SARS-CoV-2 in a community-based setting in Brazil

Sensitivity and specificity of a rapid test for assessment of exposure to SARS-CoV-2 in a community-based setting in Brazil

High-Accuracy Multiplexed SARS-CoV-2 Antibody Assay with Avidity and Saliva Capability on a Nano-Plasmonic Platform

Addressing Coronavirus Disease 2019 in Spine Surgery: A Rapid National Consensus Using the Delphi Method via Teleconference

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