Antibody responses in humans to an inactivated hantavirus vaccine (Hantavax®)

Cho, Hae-Wol; Howard, Colin R.

doi:10.1016/s0264-410x(99)00057-2

Cited by 67 publications

(45 citation statements)

References 10 publications

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“…Currently, formalin-inactivated HTNV is used as a vaccine in South Korea. However, the protective response to this vaccine is limited and requires continuous vaccination every second year to gain sufficient protection (Cho & Howard, 1999;Cho et al, 2002;Sohn et al, 2001). Reassortants between HTNV and PHV, which present the immunogenic properties of HTNV with the nonpathogenic properties of PHV, might be a promising option for the development of a novel vaccination strategy.…”

Section: Discussionmentioning

confidence: 99%

Generation and characterization of genetic reassortants between Puumala and Prospect Hill hantavirus in vitro

Handke

Oelschlegel

Franke

et al. 2010

Journal of General Virology

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Hantaviruses belong to the family Bunyaviridae characterized by tri-segmented RNA genomes. Depending on the hantavirus species, infection can lead to hantavirus cardiopulmonary or haemorrhagic fever with renal syndrome. In vitro studies suggest that pathogenic hantaviruses evade induction of innate antiviral responses, and this ability might determine the virulence in humans. Since reverse genetic systems are not available, in vitro reassortment is currently the only way to culture defined hantavirus variants. Here, we demonstrate for the first time the generation of a reassortant between a pathogenic Old World and a non-pathogenic New World hantavirus in vitro. The reassortant contained the glycoprotein coding M-segment derived from the pathogenic Puumala virus (PUUV) and the other genomic segments coding for the nucleocapsid protein and RNA-dependent RNA-polymerase from Prospect Hill virus (PHV), which is taken as nonpathogenic in humans. Exchange of the M-segment was confirmed by sequencing and virus neutralization test with PUUV-specific sera. Functional analysis of the reassortant and parental viruses revealed characteristic growth kinetics and innate immune responses as determined by expression analyses for lambda interferon and MxA, and by interferon-stimulated response element reporter gene studies. Consistent with previous studies with other pathogenic hantaviruses, PUUV elicited reduced innate responses if compared with PHV. In all these functional assays the reassortant revealed PHV-like phenotypes. Thus, neither the PUUV Msegment nor entry via specific M-segment directed pathways modulated the virus type-specific innate responses. Moreover, the data imply that this approach might be an option for production of attenuated viruses that could be used as vaccines against pathogenic hantaviruses.

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Section: Discussionmentioning

confidence: 99%

Generation and characterization of genetic reassortants between Puumala and Prospect Hill hantavirus in vitro

Handke

Oelschlegel

Franke

et al. 2010

Journal of General Virology

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“…Lung, liver, kidney, and spleen tissues were dissected using separate instruments and were stored at Ϫ80°C until the samples were used for virus isolation and RT-PCR and mtDNA analyses. Except for U.S. Army personnel, all staff engaged in the trapping of shrews and rodents and the processing of tissue samples had been vaccinated with a hantavirus vaccine (Hantavax) licensed by the Korean Food and Drug Administration (11) or possessed preexisting immunity to hantaviruses as a result of natural infection.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Imjin Virus, a Newly Isolated Hantavirus from the Ussuri White-Toothed Shrew ( Crocidura lasiura )

Song

Kang

et al. 2009

J Virol

102

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Until recently, the single known exception to the rodent-hantavirus association was Thottapalayam virus (TPMV), a long-unclassified virus isolated from the Asian house shrew (Suncus murinus)Hantaviruses (family Bunyaviridae, genus Hantavirus) are medically important rodent-borne pathogens, causing hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). The belief in long-standing coevolutionary relationships between hantaviruses and their reservoir rodent host species is supported by virus and rodent gene phylogenies. That is, phylogenetic analyses, based on full-length viral genomic sequences and rodent mitochondrial DNA (mtDNA) or nuclear gene sequences, indicate that antigenically distinct hantaviruses segregate into clades, which parallel the evolution of rodents in the subfamilies Murinae, Arvicolinae, Neotominae, and Sigmodontinae (23,25,26,28,39,54). Previously, this phylogenetic insight has been successfully employed to direct the discovery of new hantaviruses, such as those found in the Korean field mouse (Apodemus peninsulae) (5) and the royal vole (Myodes regulus) (47).Renewed interest in the role of nonrodent reservoirs in the evolution of hantaviruses has been spurred by recent analysis of the entire genome of Thottapalayam virus (TPMV), a hantavirus isolated from the Asian house shrew (Suncus murinus) (10, 61), which revealed a separate phylogenetic clade, suggesting early evolutionary divergence from rodent-borne hantaviruses (44, 56). Armed with oligonucleotide primers designed on the basis of conserved regions of the TPMV genome and guided by long-ignored reports of serologic and antigenic evidence of hantavirus infection in shrews (20, 33, 52), we have previously detected genetically distinct hantaviruses in the Eurasian common shrew (Sorex araneus) from Switzerland (45); the Chinese mole shrew (Anourosorex squamipes) from Vietnam (46); and the northern short-tailed shrew (Blarina brevicauda), masked shrew (Sorex cinereus), and dusky shrew (Sorex monticolus) from the United States (1, 2) by reverse transcription-PCR (RT-PCR). Novel hantavirus genomes in Therese's shrew (Crocidura theresae) from Guinea (29); the vagrant shrew (Sorex vagrans), Trowbridge's shrew (Sorex trowbridgii), and the American water shrew (Sorex palustris) from the United States (H. J. Kang and R. Yanagihara, unpublished data); and the flat-skulled shrew (Sorex roboratus) and Laxmann's shrew (Sorex caecutiens) from Russia (Kang and Yanagihara, unpublished) have also been detected.Here, we report the antigenic, genetic, and phylogenetic characterization of a newly identified hantavirus, designated Imjin virus (MJNV), isolated from Ussuri white-toothed shrews of the species Crocidura lasiura (order Soricomorpha, family Soricidae, subfamily Crocidurinae) captured near the demilitarized zone (DMZ) in the Republic of Korea. The discovery of MJNV and other soricid-borne hantaviruses from widely separated geographic regions, spanning four continents,

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“…Interestingly, efficacy studies with an inactivated HTNV vaccine (Hantavax) in regions where HV are endemic demonstrated a reduction in the number of HFRS cases [76]. Nevertheless, the vaccine requires optimisation in terms of induction of a long-lasting neutralising immune response [77]. To overcome the necessity of high-level safety containment for production of HV vaccines, different recombinant approaches have been followed.…”

Section: Vaccines and Antiviral Therapymentioning

confidence: 99%

Emerging Viruses: The Case ‘Hantavirus’

et al. 2002

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This review briefly summarises the recent knowledge about hantavirus infections and raises particular problems in hantavirus research that need further investigation. The following questions are addressed: (i) are hantaviruses distributed worldwide and what leads to new outbreaks, (ii) what is known about hantavirus evolution, (iii) how can hantavirus species be defined, (iv) what are the determinants of hantavirus pathogenesis in humans, and (v) what problems are associated with the development of new vaccines and antiviral therapeutics.

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Antibody responses in humans to an inactivated hantavirus vaccine (Hantavax®)

Cited by 67 publications

References 10 publications

Generation and characterization of genetic reassortants between Puumala and Prospect Hill hantavirus in vitro

Generation and characterization of genetic reassortants between Puumala and Prospect Hill hantavirus in vitro

Characterization of Imjin Virus, a Newly Isolated Hantavirus from the Ussuri White-Toothed Shrew ( Crocidura lasiura )

Emerging Viruses: The Case ‘Hantavirus’

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