Antibody responses by chinook salmon (Oncorhynchus tshawytscha) and rainbow trout (Oncorhynchus mykiss) to various protein antigens

A novel recombinant bivalent outer membrane protein of Vibrio vulnificus and Aeromonas hydrophila as a vaccine antigen of American eel (Anguilla rostrata)

“…Standard errors of antibody titers in eels of PBS group from 14 to 42 d were changeable, suggesting that the out bred nature of the fish population for this study may be different. Such phenomenon has been reported in American eel [18], Large yellow croaker [26], rainbow trout (Oncorhynchus mykiss) [40] and Atlantic salmon (Salmo salar L.) [41]. Antibody titers were also increased in PBS Control group from 14 to 28 d, which can be explained by the non-specific absorption of the ELISA plate.…”

Cloning, expression and immunogenicty analysis of five outer membrane proteins of Vibrio parahaemolyticus zj2003

“…Such phenomenon has been reported in Chinook salmon (Oncorhynchus tshawytscha), rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar L.), in these species, both the frequencies and the antibody level of the responses can by highly variable [35,36]. In our study, though antibody levels to various protein antigen seemed increase during the experiment period, the tendency was not always constant, the variety of the antibody response of individuals from the same group and the small sample (n ¼ 5) may take account for it.…”

Effect of dietary vitamin A deficiency on growth performance, feed utilization and immune responses of juvenile Jian carp (Cyprinus carpio var. Jian)

“…Sera were separated and inactivated by placing them in a 56 °C water bath for 30 min, and stored at −80 °C. Antibody was determined by an enzyme‐linked immunosorbent assay (Alcorn & Pascho 2002).…”

“…Blood samples were allowed to clot overnight at 4 1C and centrifuged for 15 min at 3000 Â g. Sera were separated and inactivated by placing them in a 56 1C water bath for 30 min, and stored at À 80 1C. Antibody was determined by an enzyme-linked immunosorbent assay (Alcorn & Pascho 2002). All data are presented as means AE standard deviation.…”