Antibody response to Staphylococcus aureus collagen binding protein in patients with S. aureus septicaemia and collagen binding properties of corresponding strains

Abstract

Summary. An ELISA was developed for the detection of IgG antibodies to StaphyZococcus uureus collagen binding protein (CnBP) in 95 patients with S. aureus endocarditis, complicated septicaemia with bone and joint involvement or uncomplicated septicaemia and in 95 control patients. Sixty percent of S. aureus-infected patients showed a positive peak anti-CnBP antibody level or a significant rise in titre, or both, during infection, but patients with S . aureus endocarditis or complicated septicaemia could not be differentiated from those with uncomplicated S. aureus septicaemia. The collagen binding capacity of S. aureus strains from 82 of the 95 patients was investigated in a particle agglutination assay and a 1251-labelled assay. All strains bound collagen in the particle agglutination assay as did 68 YO in the '"I-labelled assay, but there was no correlation between collagen binding of the patient strain and endocarditis, joint or skeletal involvement. An anti-CnBP antibody response was seen in 16 patients infected with a S. aureus strain negative for collagen binding in vitro, indicating in-vivo expression of CnBP.

Towards the immune proteome of Staphylococcus aureus – The anti-S. aureus antibody response

“…Several studies show that antibodies against S. aureus antigens are generated or boosted during S. aureus infection (Table 1) (Colque-Navarro et al, 1998Dryla et al, 2005;Lorenz et al, 2000;Ryding et al, 1995). But how much do they contribute to protection?…”

Distinctive patterns in the human antibody response to Staphylococcus aureus bacteremia in carriers and non‐carriers

“…We propose that most anti-S. aureus antibody responses are elicited by invasion rather than mere carriage. In fact, average concentrations of antibodies binding to a number of selected S. aureus antigens increase during infection [27][28][29][30][31][32]. Different from non-carriers and intermittent carriers, persistent carriers may be exposed to the same strain for a long time, and it appears likely that they experience repeated minor invasive episodes.…”

Transcriptional regulation of the Staphylococcus aureus collagen adhesion gene, cna

“…Among the surface proteins produced by S. aureus are a diverse group of adhesins capable of binding host proteins present in the extracellular matrix. Although S. aureus can bind a variety of host proteins (16), isolates associated with musculoskeletal disease generally exhibit an enhanced propensity to bind collagen and/or bone sialoprotein (3,12,(20)(21)(22). This observation is particularly relevant in light of the fact that most strains do not bind either of these matrix molecules (9,20,21).…”