Antibody Response to Pneumococcal Polysaccharide Vaccine in Young, Adult and Old Mice

Aaberge, Ingeborg S.; North, Robert J.; Groeng, Else‐Carin; Løvik, Martinus

doi:10.1111/j.1365-3083.1993.tb01689.x

Cited by 8 publications

(17 citation statements)

References 26 publications

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“…In contrast to Ficoll PS, purified pneumococcal PS induced almost exclusively IgM responses. Although the weak or absent IgG responses to Pn‐23 immunization observed in adult BALB/c mice do not correlate to the situation in human adults, where IgG responses can be generated by the same Pn‐23 vaccine, they are in accordance with results obtained in human infants as well as in CBA/J mice 32 . Unexpectedly, CpG‐ODN co‐administration failed to enhance either IgM or IgG responses to unconjugated SPnPS.…”

Section: Discussionsupporting

confidence: 55%

“…Although the weak or absent IgG responses to Pn-23 immunization observed in adult BALB/c mice do not correlate to the situation in human adults, where IgG responses can be generated by the same Pn-23 vaccine, they are in accordance with results obtained in human infants as well as in CBA/J mice. 32 Unexpectedly, CpG-ODN co-administration failed to enhance either IgM or IgG responses to unconjugated SPnPS. This failure could have been due to interference of SPnPS on CpG-ODN mediated B-cell activation, but this phenomenon was not observed even when high concentrations of SPnPS were added during in vitro incubation of splenocytes with CpG-ODN.…”

Section: Discussionmentioning

confidence: 96%

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Adjuvant effects of CpG oligodeoxynucleotides on responses against T‐independent type 2 antigens

et al. 2001

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Oligodeoxynucleotides containing CpG motifs (CpG-ODN) are potent in vitro B-cell activators and they have been successfully used to increase in vivo antibody responses to T-dependent peptide and protein antigens. In contrast, the use of CpG-ODN to enhance in vivo antibody responses to various T-independent type 2 (TI-2) antigens has recently generated contradictory results. In this study, we compared the CpG-ODN stimulatory effect on antibody responses of adult and young BALB/c mice to trinitrophenylaminoethyl-carboxymethyl (TNP) -Ficoll and to polysaccharides (PS) from several distinct serotypes of Streptococcus pneumoniae (SPn). CpG-ODN co-administration significantly enhanced antigen-specific immunoglobulin M (IgM), IgG, IgG1 and IgG2a titres to TNP-Ficoll. The depletion of CD4+ cells by monoclonal antibodies (GK1.5) identified their essential role in CpG-ODN-mediated enhancement of antibody responses. In contrast to TNP-Ficoll, CpG-ODN failed to enhance IgM and IgG responses to any of the 18 SPnPS serotypes tested. Providing T-cell epitopes by the conjugation of SPnPS to the carrier protein tetanus toxoid again allowed CpG-ODN to mediate enhancement of IgG, IgG2a and IgG3 responses to most SPnPS serotypes. Thus, antigen-presenting cell/T-cell interaction appears to largely mediate the in vivo influence of CpG-ODN on antibody responses to TI-2 antigens. In early life, additional factors limit CpG-ODN modulation of antibody responses to TI-2 antigens.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 96%

Adjuvant effects of CpG oligodeoxynucleotides on responses against T‐independent type 2 antigens

et al. 2001

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“…Piasma was stored at ~20''C until analysed. IgM, IgG and IgA antibodies to pneumococcal polysaccharide vaccine and to single pneumococcal polysaccharides (American Type Culture Collection, Rockville, MD, USA) were determined by an ELISA technique as described previously [5]. Briefly, microtest plates (Nunc, Roskilde, Denmark) were coated with pneumococcal polysaccharide vaccine or pneumococcal polysaccharide type 1, 3, 4, 7F, 14 or 19F.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously reported experiments on the primary antibody response to pneumococcal polysaccharide antigens given in the form of a polyvalent vaccine in mice [5]. We now report experiments in which the antibody response after reimmunization with polyvalent pneumococcal polysaccharide vaccine was examined.…”

Section: Introductionmentioning

confidence: 99%

The Antibody Response to Secondary Immunization with Pneumococcal Polysaccharides in Mice

Aaberge¹,

Løvik²

1995

Scand J Immunol

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The benefit of re-immunization with pneumococcal polysaccharide vaccine is an important question in clinical practice. In an experimental model, BALB/c and CBA/J mice were re-immunized s.c. with a 23-valent pneumococcal polysaccharide vaccine at various time intervals after a first immunization with the same vaccine. The antibody response after the secondary immunization showed similar kinetics as after primary immunization, and was mainly an IgM antibody response. Re-immunization at 28 days or earlier induced a decrease in the serum antibody levels to the vaccine. Reimmunization at 120 days or later induced higher antibody levels than after the first immunization. Significant increases in antibody levels to serotypes 1, 4, 7F and 19F out of six serotypes tested were observed. In CBA/J mice, but not in BALB/c mice, the dose used for primary immunization appeared to influence the magnitude of the antibody response to secondary immunization. Our results indicate that the time interval between primary and secondary immunization is an important determinant with regard to the magnitude of the antibody response to re-immunization with pneumococcal polysaccharide vaccine.

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“…Most earlier studies of the immune response to bacterial respiratory tract pathogens have been performed using immunization routes remote from the respiratory tract. It has been shown in both animal models [1][2][3] and in man [4 7] that immunization with purified pneumococcal capsular polysaccharides (PPS) gives a protective antibody response. In a recent study it was shown that parenteral im munization of adults using PPS or PPS conjugated to me ningococcal outer membrane protein gave both a mucosal and a systemic antibody response [8].…”

Section: Introductionmentioning

confidence: 99%

Antibody Response in Bronchoalveolar Lavage and Serum of Rats after Aerosol Immunization of the Airways with a Well-Adhering and a Poorly Adhering Strain of <i>Streptococcus pneumoniae</i>

Arvå

Dahlgren²,

Lock³

et al. 1996

Int Arch Allergy Immunol

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This study describes the antibody response to two bacterial antigens, pneumolysin toxoid (PL) and purified pneumococcal capsular polysaccharide (PPS) 19F, in bronchoalveolar lavage (BAL) and in serum in rats after aerosol immunization with whole killed Streptococcus pneumoniae. To study the importance of bacterial adherence for antibody formation, one well-adhering and one poorly adhering strain of S. pneumoniae was used. The results show local specific anti-PPS 19F IgA, IgM and IgG antibody activities after aerosol immunization. Anti-PL antibody activity in all three immunoglobulin classes was found, although the anti-PL activity was lower than the anti-PPS 19F antibody activity. The IgA anti-PPS 19F antibody activity in BAL after immunization with the well-adhering strain was higher than with the poorly adhering strain. We conclude that aerosol immunization with S. pneumoniae induces a local, specific antibody production in the lung of the rat.

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Antibody Response to Pneumococcal Polysaccharide Vaccine in Young, Adult and Old Mice

Cited by 8 publications

References 26 publications

Adjuvant effects of CpG oligodeoxynucleotides on responses against T‐independent type 2 antigens

Adjuvant effects of CpG oligodeoxynucleotides on responses against T‐independent type 2 antigens

The Antibody Response to Secondary Immunization with Pneumococcal Polysaccharides in Mice

Antibody Response in Bronchoalveolar Lavage and Serum of Rats after Aerosol Immunization of the Airways with a Well-Adhering and a Poorly Adhering Strain of <i>Streptococcus pneumoniae</i>

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