Antibody response to immunization with influenza A/USSR/77 (H1N1) virus in young individuals primed or unprimed for A/New Jersey/76 (H1N1) virus

Masurel, N.; Ophof, P.; Jong, Ptvm De

doi:10.1017/s0022172400069412

Cited by 69 publications

(31 citation statements)

References 13 publications

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“…Plasma samples were stored at Ϫ20°C and tested for the presence of influenza A virus (H3N2 and H1N1)-specific Abs in a hemagglutination inhibition (HAI) assay according to standard methods (21,22) using turkey erythrocytes and four hemagglutinating units of virus. The sera were tested for Abs against 11 vaccine strains of subtype H3N2 and 6 vaccine strains of the H1N1 subtype used since 1968.…”

Section: Serologymentioning

confidence: 99%

Recognition of Homo- and Heterosubtypic Variants of Influenza A Viruses by Human CD8+ T Lymphocytes

Boon

Mutsert

Baarle

et al. 2004

The Journal of Immunology

114

105

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In the present study, the recognition of epitope variants of influenza A viruses by human CTL was investigated. To this end, human CD8+ CTL clones, specific for natural variants of the HLA-B*3501-restricted epitope in the nucleoprotein (NP418–426), were generated. As determined in 51Cr release assays and by flow cytometry with HLA-B*3501-peptide tetrameric complexes, CTL clones were found to be specific for epitopes within one subtype or cross-reactive with heterosubtypic variants of the epitope. Using eight natural variants of the epitope, positions in the 9-mer important for T cell recognition and involved in escape from CTL immunity were identified and visualized using multidimensional scaling. It was shown that positions 4 and 5 in the 9-mer epitope were important determinants of T cell specificity. The in vivo existence of CD8+ cells cross-reactive with homo- and heterosubtypic variants of the epitope was further confirmed using polyclonal T cell populations obtained after stimulation of PBMC with different influenza A viruses. Based on the observed recognition patterns of the clonal and polyclonal T cell populations and serology, it is hypothesized that consecutive infections with influenza viruses containing different variants of the epitope select for cross-reactive T cells in vivo.

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Section: Serologymentioning

confidence: 99%

Recognition of Homo- and Heterosubtypic Variants of Influenza A Viruses by Human CD8+ T Lymphocytes

Boon

Mutsert

Baarle

et al. 2004

The Journal of Immunology

114

105

View full text Add to dashboard Cite

show abstract

“…Plasma samples were stored at Ϫ20°C and tested for the presence of influenza A virus (H3N2)-and influenza B virus-specific Abs in a hemagglutination inhibition assay according to standard methods (19,20) using turkey erythrocytes and four hemagglutinating units of virus. The sera were tested for Abs against 11 vaccine strains of subtype H3N2 used since 1968 and 11 influenza B virus strains (from 1979 till 2001).…”

Section: Serologymentioning

confidence: 99%

Preferential HLA Usage in the Influenza Virus-Specific CTL Response

Boon

Mutsert

Fouchier

et al. 2004

The Journal of Immunology

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To study whether individual HLA class I alleles are used preferentially or equally in human virus-specific CTL responses, the contribution of individual HLA-A and -B alleles to the human influenza virus-specific CTL response was investigated. To this end, PBMC were obtained from three groups of HLA-A and -B identical blood donors and stimulated with influenza virus. In the virus-specific CD8+ T cell population, the proportion of IFN-γ- and TNF-α-producing cells, restricted by individual HLA-A and -B alleles, was determined using virus-infected C1R cells expressing a single HLA-A or -B allele for restimulation of these cells. In HLA-B*2705- and HLA-B*3501-positive individuals, these alleles were preferentially used in the influenza A virus-specific CTL response, while the contribution of HLA-B*0801 and HLA-A*0101 was minor in these donors. The magnitude of the HLA-B*0801-restricted response was even lower in the presence of HLA-B*2705. C1R cells expressing HLA-B*2705, HLA-A*0101, or HLA-A*0201 were preferentially lysed by virus-specific CD8+ T cells. In contrast, the CTL response to influenza B virus was mainly directed toward HLA-B*0801-restricted epitopes. Thus, the preferential use of HLA alleles depended on the virus studied.

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“…Serum haemagglutination inhibition (HI) titres were determined twice by standard methods [15] simultaneously in pre-and post-vaccination sera. Titres were expressed as reciprocals of the dilution showing 50% haemagglutination inhibition with 3 haemagglutination units of the antigen.…”

Section: Laboratory Investigations and Calculationsmentioning

confidence: 99%

Humoral immune response and delayed type hypersensitivity to influenza vaccine in patients with diabetes mellitus

Diepersloot

Bouter

Beyer³

et al. 1987

Diabetologia

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Summary.The antibody response and delayed type hypersensitivity reaction to commercially available trivalent influenza vaccine in 159 patients with diabetes mellitus was compared with response and reaction in 28 healthy volunteers. A correction for prevaccination titres was made. No differences were found between diabetic patients and control subjects with respect to antibody response to the three vaccine strains as measured by the difference between geometric mean titres of post-and prevaccination sera. In Type 1 (insulin-dependent) diabetic patients the incidence of non-responders to two vaccine components was significantly increased (p< 0.05). The delayed type hypersensitivity reaction to influenza antigen was significantly decreased in patients with high concentrations of glycosylated haemoglobin (p<0.01). These findings suggest a role for impaired immune response in the increased influenza morbidity and mortality in patients with diabetes mellitus. Implications for therapy and vaccination strategy are discussed.

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Antibody response to immunization with influenza A/USSR/77 (H1N1) virus in young individuals primed or unprimed for A/New Jersey/76 (H1N1) virus

Cited by 69 publications

References 13 publications

Recognition of Homo- and Heterosubtypic Variants of Influenza A Viruses by Human CD8+ T Lymphocytes

Recognition of Homo- and Heterosubtypic Variants of Influenza A Viruses by Human CD8+ T Lymphocytes

Preferential HLA Usage in the Influenza Virus-Specific CTL Response

Humoral immune response and delayed type hypersensitivity to influenza vaccine in patients with diabetes mellitus

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