Antibody response to allelic variants of 19kDa fragment of MSP-1: Recognition of a variant and protection associated with ethnicity in Assam, India

Lourembam, Sonia D.; Baruah, Satyabrat Malla Bujar

doi:10.1016/j.vaccine.2011.11.069

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…Despite the complexity of the cellular and humoral immune response induced by Mtb, serological markers to distinguish between healthy and sick individuals remains attractive to researchers around the world [52] . The known protective immune response against TB is the predominant T cell response.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity of a Fusion Protein Containing Immunodominant Epitopes of Ag85C, MPT51, and HspX from Mycobacterium tuberculosis in Mice and Active TB Infection

et al. 2012

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Tuberculosis (TB) remains a major global health problem. The only vaccine against tuberculosis, attenuated Mycobacterium bovis Bacillus Calmette-Guerin (BCG), has demonstrated relatively low efficacy and does not provide satisfactory protection against the disease in adults. More effective vaccines and better therapies are urgently needed to reduce the global spread of TB. This study evaluated the immunogenicity of a recombinant M. tuberculosis Ag85C-MPT51-HspX fusion protein (CMX) in mice and individuals with active tuberculosis. BALB/c mice were immunized with the CMX protein liposome-encapsulated with CpG DNA or with CpGDNA liposome-encapsulated, liposome or saline as negative controls. The immunization produced high levels of anti-CMX -specific IgG1 and IgG2a antibodies and induced an increase in the relative and absolute numbers of specific TCD4 IFN-γ+ and TNF-α+ cells in the spleen. Sera from a cohort of individuals with active tuberculosis contained higher levels of IgG and IgM that recognized CMX when compared to healthy individuals. In conclusion, this protein was shown to be immunogenic both in mice and humans.

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Section: Discussionmentioning

confidence: 99%

Immunogenicity of a Fusion Protein Containing Immunodominant Epitopes of Ag85C, MPT51, and HspX from Mycobacterium tuberculosis in Mice and Active TB Infection

et al. 2012

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“…Higher responses were found against: Helicobacter pylori in non-Japanese Brazilians than Japanese Brazilians [17]; Streptococcus pyogenes superantigen in Polynesian than New Zealand Europeans [18]; Plasmodium falciparum in Fulani than sympatric ethnic groups in Burkina Faso [19]; P. falciparum in Austro-Asiatic than Tibeto-Burman groups in north-east India [20]. Jensen et al [21] reported comparatively higher anti- Plasmodium titres in subjects from Flores (Indonesia) than in counterparts from southern Sudan.…”

Section: Discussionmentioning

confidence: 99%

Significantly Lower Anti-Leishmania IgG Responses in Sudanese versus Indian Visceral Leishmaniasis

et al. 2014

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BackgroundVisceral leishmaniasis (VL), a widely distributed systemic disease caused by infection with the Leishmania donovani complex (L. donovani and L. infantum), is almost always fatal if symptomatic and untreated. A rapid point-of-care diagnostic test for anti-Leishmania antibodies, the rK39-immunochromatographic test (rK39-ICT), has high sensitivity and specificity in South Asia but is less sensitive in East Africa. One of the underlying reasons may be continent-specific molecular diversity in the rK39 antigen within the L. donovani complex. However, a second reason may be differences in specific IgG anti-Leishmania levels in patients from different geographical regions, either due to variable antigenicity or immunological response.Methodology/Principal FindingsWe determined IgG titres of Indian and Sudanese VL patients against whole cell lysates of Indian and Sudanese L. donovani strains. Indian VL patients had significantly higher IgG titres against both L. donovani strains compared to Sudanese VL patients (p<0.0001). Mean reciprocal log10 50% end-point titres (1/log10t50) were i) 3.80 and 3.88 for Indian plasma and ii) 2.13 and 2.09 for Sudanese plasma against Indian and Sudanese antigen respectively (p<0.0001). Overall, the Indian VL patients therefore showed a 46.8–61.7 -fold higher mean ELISA titre than the Sudanese VL patients. The higher IgG titres occurred in children (<16 years old) and adults of either sex from India (mean 1/log10t50: 3.60–4.15) versus Sudan (mean 1/log10t50: 1.88–2.54). The greatest difference in IgG responses was between male Indian and Sudanese VL patients of ≥ 16 years old (mean 1/log10t50: 4.15 versus 1.99 = 144-fold (p<0.0001).Conclusions/SignificanceAnti-Leishmania IgG responses among VL patients in Sudan were significantly lower than in India; this may be due to chronic malnutrition with Zn2+ deficiency, or variable antigenicity and capacity to generate IgG responses to Leishmania antigens. Such differential anti-Leishmania IgG levels may contribute to lower sensitivity of the rK39-ICT in East Africa.

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“…This sequence homology of the two antigens leading to B cells with common B cell epitopes results in production of cross reactive antibodies. The presence of different Plasmodium variants may also lead to variant specific antibody (Lourembam and Baruah 2012;Putaporntip et al 2002;Shi et al 1996;Soares et al 1999a).…”

Section: Discussionmentioning

confidence: 99%

Acquired antibody responses against merozoite surface protein-119 antigen during Plasmodium falciparum and P.vivax infections in South Indian city of Mangaluru

et al. 2020

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Merozoite surface protein-1 (MSP-1) of malaria parasites has been extensively studied as a malaria vaccine candidate and the antibody response to this protein is an important indicator of protective immunity to malaria. Mangaluru city and its surrounding areas in southwestern India are endemic to malaria with Plasmodium vivax being the most widespread and prevalent species although P. falciparum also frequently infects. However, no information is available on the level of protective immunity in this population. In this regard, a prospective hospital-based study was performed in malarial patients to assess antibody responses against the 19-kDa C-terminal portion of P. vivax and P. falciparum MSP-1 (MSP-1 19 ). Serum samples from 51 healthy endemic controls and 267 infected individuals were collected and anti-MSP-1 19 antibody levels were analyzed by ELISA. The possible association between the antibody responses and morbidity parameters such as malarial anemia and thrombocytopenia was investigated. Among the 267 infected cases, 144 had P. vivax and 123 had P. falciparum infections. Significant levels of anti-MSP-1 19 antibody were observed both in P. vivax (123/144; 85.4%) and P . falciparum (108/123; 87.9%) infected individuals. In both type of infections, the major antibody isotypes were IgG1 and IgG3. The IgG levels were found to be increased in patients with severe anemia and thrombocytopenia. The antibody levels were also higher in infected individuals who had several previous infections, although antibodies produced during previous infections were short lived. The predominance of cytophilic anti-MSP-1 19 IgG1 and IgG3 antibodies suggests the possibility of a dual role of Pv MSP-1 19 and Pf MSP-1 19 during malarial immunity and pathogenesis.

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Antibody response to allelic variants of 19kDa fragment of MSP-1: Recognition of a variant and protection associated with ethnicity in Assam, India

Cited by 7 publications

References 33 publications

Immunogenicity of a Fusion Protein Containing Immunodominant Epitopes of Ag85C, MPT51, and HspX from Mycobacterium tuberculosis in Mice and Active TB Infection

Immunogenicity of a Fusion Protein Containing Immunodominant Epitopes of Ag85C, MPT51, and HspX from Mycobacterium tuberculosis in Mice and Active TB Infection

Significantly Lower Anti-Leishmania IgG Responses in Sudanese versus Indian Visceral Leishmaniasis

Acquired antibody responses against merozoite surface protein-119 antigen during Plasmodium falciparum and P.vivax infections in South Indian city of Mangaluru

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