Antibody response patterns in chikungunya febrile phase predict protection versus progression to chronic arthritis

Nayak, Kaustuv; Jain, Vineet; Kaur, Manpreet; Khan, Naushad; Gottimukkala, Kamalvishnu; Aggarwal, Charu C.; Sagar, Rohit; Gupta, Shipra; Chandra, Ramesh; Dixit, Kritika; Islamuddin, Mohammad; Khan, Wajihul Hasan; Maheshwari, Deepti; Chawla, Yadya M; Reddy, Elluri Seetharami; Panda, Harekrushna; Sharma, Prerana; Bhatnagar, Priya; Singh, Prabhat Kumar; B, Siva Raghavendhar; Patel, Ashok Kumar; Ratageri, Vinod H.; Chandele, Anmol; Ray, Pratima

doi:10.1172/jci.insight.130509

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…Of the 36 samples, we found 18 with detectable neutralizing antibodies to CHIKV (2/17 from the IgM+IgG− subgroup and 16/19 from the IgM+IgG+ subgroup) ( Table 1 , Figure 2a ). The lack of neutralizing antibodies in participants from the CHIKV IgM+IgG− subgroup (15/17) was unexpected, but was in agreement with the observation of five distinct antibody patterns and the presence of an IgM+IgG− period in some individuals based on the study of 133 samples following acute CHIKV infection [ 23 ].…”

Section: Resultssupporting

confidence: 80%

“…After CHIKV infection, individuals develop an IgM response starting from 3 to 8 days post-symptom onset, followed by an IgG response at 7 to 14 days [ 25 ]. A recent study reported five distinct antibody patterns (IgM−IgG−/NT−, IgM+IgG−/NT−, IgM+IgG−/NT+, IgM+IgG+/NT− and IgM+IgG+/NT+) during acute febrile phase and the presence of NT or IgG antibody was associated with protection against developing chronic arthritis in the future, which was supported by two studies of outbreaks in India (2010–3 and 2014–6) [ 23 , 26 ]. Although most individuals of the IgM−IgG− and IgM+ IgG− subgroups developed IgG and NT antibodies at the convalescent-phase, this may explain some IgM+IgG− and NT− samples observed in our study.…”

Section: Discussionmentioning

confidence: 77%

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Chikungunya virus antepartum transmission and abnormal infant outcomes in a cohort of pregnant women in Nigeria

Sagay,

Hsieh,

Dai

et al. 2024

International Journal of Infectious Diseases

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Section: Resultssupporting

confidence: 80%

Section: Discussionmentioning

confidence: 77%

Chikungunya virus antepartum transmission and abnormal infant outcomes in a cohort of pregnant women in Nigeria

Sagay,

Hsieh,

Dai

et al. 2024

International Journal of Infectious Diseases

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“…8 On the contrary, induction of IgG1 and IgG2 in acute-phase plasma samples with negligible IgG3 and no IgG4 was found when screened with CHIKV. 9 Taken together, when CHIKV was used for ELISA, both Indian studies identified IgG1 (Nayak et al 9 and present study), although IgG2 and IgG4 subtype detection rates did vary; IgG3 was the predominant serotype with envelope protein peptides. 8 The possibility of unavailability of IgG3 reactive peptide epitopes on the surface of the virus cannot be ruled out.…”

Section: Discussionsupporting

confidence: 53%

“…8 Contrary to these finding, IgG1 and IgG2 antibodies were detected in acutephase serum samples with negligible IgG3 and no IgG4. 9 It is well reported that antiviral activity (virus neutralization and clearance) is associated with IgG1 and IgG3 subtypes that bind to all human Fc-gamma receptors (FcγR) classes and target antigen to phagocytosis. 10 Both these antibodies are elicited against protein antigens and are outcome of Th1 response.…”

Section: Introductionmentioning

confidence: 99%

Age-Dependent Evaluation of Immunoglobulin G Response after Chikungunya Virus Infection

Patil

Gosavi

Mishra

et al. 2021

The American Journal of Tropical Medicine and Hygiene

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Current chikungunya antibody prevalence and titers are likely to differ based on exposure rates before the 2006 reemergence. For vaccine usage, such data are of immense importance. This study addresses age-stratified IgG titers and its subtypes in Pune, India, endemic for the disease. One hundred seventy serum pools (791 individuals with prior chikungunya exposure, age stratified) from exposed and 15 samples from acute disease phase were screened. Inactivated chikungunya virus (CHIKV)–based indirect ELISA was used to determine anti–CHIKV-IgG and its subtypes. Neutralizing antibody titers (plaque reduction neutralization test [PRNT]) were compared with binding antibody titers (ELISA). Anti–CHIKV-IgG titers along with IgG1 and IgG4 increased till the age-group of 11–15 years and remained comparable thereafter till > 65 years. IgG1 was the predominant IgG subtype detected in all the pools, whereas IgG4 was present in 151/170 pools. Strong correlation of IgG1 was obtained with CHIKV–PRNT50 titers. None of the sample had anti–CHIKV-IgG2, whereas five pools had IgG3 antibody. In the acute-phase serum sample, IgG1 was present in all the samples, whereas IgG4 was present in 8/15 samples. IgG4 was predominant in four samples. During acute phase and at different times postinfection, IgG1 circulated in high titers followed by IgG4. Higher antibody titers in adults reflect reexposures. The data will prove useful in assessing immune response to CHIKV vaccine.

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“…The antibody response patterns might vary between mono-infected and co-infected patients. Hence, it is very important to determine the mono-infection and coinfection status of the patients as the antibodies patterns generated in response to the infection might serve as biomarkers for disease progression (Nayak et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Molecular surveillance of Dengue Virus (DENV) and its co-infection with Chikungunya Virus (CHIKV) among febrile patients: A comparative study from South Delhi, India

Sagar

Raghavendhar²,

Nayak

et al. 2021

JANS

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Dengue and Chikungunya are two major arboviral infections transmitted worldwide by the mosquitoes, Aedes aegypti and Ae. albopictus. India suffers enormously with both Dengue and Chikungunya as they pose a great public health challenge. The present study aims to evaluate the prevalence of Dengue Virus (DENV), Chikungunya Virus (CHIKV) and DENV/CHIKV co-infection (by Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR)/Enzyme Linked Immunosorbent Assay (ELISA), their clinical features, DENV serotypes and CHIKV specific Immunoglobulin G (IgG) within a 7 years gap in the Delhi population. The study sample included clinically suspected febrile patients (?7 days) sera collected during 2017-2018 (n=87) and during 2008-2010 (n=623) from Delhi. Captured ELISA was performed for CHIKV IgG screening and nested PCR was done for DENV serotyping. The percentage prevalence for DENV was significantly higher than CHIKV with 41.38% (n=87) and 16.1% (n=87), respectively; interestingly, DENV/CHIKV co-infection was detected in 10.34% (n=9/87) cases during 2017-2018. Similarly, a high DENV prevalence was observed during 2008-2010 with the prevalence rate of 38.3% (69/180), 34.65% (35/101) and 47.07% (161/342), respectively. DENV 1 and DENV 3 were dominant serotype during 2008-2010 and 2017-2018 respectively. We have noticed a high prevalence (36.67%, 22/60) of the CHIKV IgG antibody in the 2017-2018 samples. Joint pain was more preferential to CHIKV mono-infection and DENV/CHIKV co-infection compared to DENV mono-infection. The present study highlights the need for active surveillance simultaneously for both DENV and CHIKV and to evaluate the role of CHIKV/DENV co-infections in disease severity in the endemic regions.

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Antibody response patterns in chikungunya febrile phase predict protection versus progression to chronic arthritis

Cited by 10 publications

References 31 publications

Chikungunya virus antepartum transmission and abnormal infant outcomes in a cohort of pregnant women in Nigeria

Chikungunya virus antepartum transmission and abnormal infant outcomes in a cohort of pregnant women in Nigeria

Age-Dependent Evaluation of Immunoglobulin G Response after Chikungunya Virus Infection

Molecular surveillance of Dengue Virus (DENV) and its co-infection with Chikungunya Virus (CHIKV) among febrile patients: A comparative study from South Delhi, India

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