Antibody Response in Rabbits Following Intranasal and Parenteral Administration of Birch Pollen

Belin, Lars; Strannegård, Ö.

doi:10.1159/000230558

Cited by 14 publications

(10 citation statements)

References 17 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rabbits immunized intranasally produced hemagglutinating antibodies to the non-allergenic fractions, but reaginic antibodies only to the allergenic fractions of birch pollen (6). As expected, IgG ab responses to the allergenic fractions were significantly higher in the hyposensitized group than in the untreated one.…”

Section: Discussionsupporting

confidence: 71%

“…Belin & Strannegard (6) demonstrated that in rabbits transmucosal immunization resulted in formation of reaginic antibodies only against allergenic antigens of birch pollen, while both the allergenic and the non-0105-4538/80/010057-08 S02.50/0 allergenic antigens induced immediate hypersensitivity when administered parenterally. In the present work immune response against fractionated antigens of birch pollen was studied in man.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Humoral and Cell‐Mediated Immune Responses to Fractions of Birch Pollen Extract

Viander

1980

Allergy

View full text Add to dashboard Cite

IgE and IgG antibodies (ab) and lymphocyte transformation (LT) were studied in untreated and hyposensitized birch pollen allergic subjects and in non-atopic controls using whole extract and fractions obtained by gel filtration of birch pollen extract. All the allergic subjects had positive IgE ab, IgG ab and LT responses to the whole extract. Both the untreated and the hyposensitized subjects had peak IgE ab and LT responses against the allergenic fractions of the extract, while negative responses were obtained in the non-atopic controls. Only hyposensitized subjects had developed high IgG ab responses to the allergenic fractions. Most of the treated and untreated subjects showed IgG ab and LT responses to the high molecular weight fractions with low allergenic activity. Significantly higher IgE ab responses to these fractions were observed in the treated subjects than in the untreated ones, indicating potentiation of IgE ab responses against some antigens during immunotherapy. Some of the allergic subjects also responded to the fractions of low molecular size (mol. wt. 2000-5000) with low allergenic activity. Both IgE ab, IgG ab and LT responses to these fractions were observed.

show abstract

Section: Discussionsupporting

confidence: 71%

mentioning

confidence: 99%

Humoral and Cell‐Mediated Immune Responses to Fractions of Birch Pollen Extract

Viander

1980

Allergy

View full text Add to dashboard Cite

show abstract

“…It has recently been shown that the non-allergenic thermolabile BP antigens are equally capable of evoking immediate skin reactions in rabbits as are the ther mostable allergenic antigens, provided the animals are immunized by a parenteral route [4]. On the other hand, intranasal administration of BP resulted in development of immediate skin reactivity only to the heat stable, allergenic BP antigens.…”

Section: Discussionmentioning

confidence: 99%

Separation and Characterization of Birch Pollen Antigens with Special Reference to the Allergenic Components

Belin¹

1972

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

The major allergens of birch pollen were separated from most of the non-allergenic antigens as well as from non-proteinic water-soluble components by gel filtration on Sephadex G-100. These allergens appeared homogenous as to their molecular weight which was calculated to be about 20,000. They seemed to consist of at least two, heat stable, differently charged but antigenically similar proteins. The major allergen of alder pollen appeared closely related to those of birch pollen. The results bring up the question of the nature of allergenicity. They are consistent with the notion that a stable structure, a certain molecular weight and ability to induce reagin formation are prerequisites for an allergenic molecule.

show abstract

“…As shown earlier [Strannegard and Yurchision, 1969b;Stranne gard, 1971;Belin and Strannegard, 1971] it was possible to induce reagin formation by administration of antigen via different routes. It was of particular interest that such low doses as 1 fig Hey, given intrave nously, or 30 fig birch pollen administered repeatedly intranasally, were able to induce reagin production.…”

Section: Discussionmentioning

confidence: 75%

Antigen Dose Dependence of Reagin Formation in Rabbits

Strannegård¹

1973

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

The effect of antigen dosage on the formation of reagins in rabbits was studied using various routes of immunization. Upon immunization with adjuvant-incorporated haemocyanin, very high and very low antigen doses failed to induce a detectable reagin response although other antibodies were produced. Similar dose-response curves were obtained after intravenous injection of bovine y-globulin. Intravenous injection of a low dose of haemocyanin resulted in a reagin response, initially in the absence of demonstrable IgG or IgM antibodies. Upon stimulation with polymerized bovine γ-globulin it was again found that high or low doses of antigen induced reagin formation poorly. Polymerization of the antigen appeared to enhance its ability to induce reagin production at high antigen dosage, but to diminish this ability at low antigen dosage. Development of immediate hypersensitivity was observed after intranasal administration of low doses of birch pollen. The results explain previous contradictory reports as to dose dependence in reagin formation. They suggest that some reagin-forming cell precursors have high affinity for antigen and that the apparent unresponsiveness to high antigen doses may be due to consumption of circulating reagins by antigens. Although polymerization of an antigen may make it more immunogenic, the allergenicity of a substance is probably not markedly enhanced if it occurs in particulate rather than soluble form.

show abstract

Antibody Response in Rabbits Following Intranasal and Parenteral Administration of Birch Pollen

Cited by 14 publications

References 17 publications

Humoral and Cell‐Mediated Immune Responses to Fractions of Birch Pollen Extract

Humoral and Cell‐Mediated Immune Responses to Fractions of Birch Pollen Extract

Separation and Characterization of Birch Pollen Antigens with Special Reference to the Allergenic Components

Antigen Dose Dependence of Reagin Formation in Rabbits

Contact Info

Product

Resources

About