Antibody Response in Primary Human Immunodeficiency Virus Infection

Gaines, Hans; Sönnerborg, Anders; Czajkowski, Jan; Chiodi, Francesca; Fenyö, Eva-Maria; Sydow, M von; Albert, Jan; Pehrson, P; Moberg, Lars; Åsjö, Birgitta; Forsgren, Marianne

doi:10.1016/s0140-6736(87)92696-1

Cited by 171 publications

(57 citation statements)

References 17 publications

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“…The same sera that previously recognized Env proteins in RIPA did not detect these proteins in an immunoblot assay. Similar observations have been made by others for foamy viruses (Giron et al, 1993) and HIV Gaines et al, 1987).…”

Section: Discussionsupporting

confidence: 90%

Reactivity of primate sera to foamy virus Gag and Bet proteins

Hahn¹,

Baunach²,

Bräutigam³

et al. 1994

Journal of General Virology

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In order to establish criteria for the serodiagnosis of foamy virus infections we investigated the extent to which sera from infected individuals of human and primate origin react with structural and non-structural virus proteins in immunoblot assays. Using lysates from infected cells as the source of virus antigen, antibodies were preferentially detected against the Gag proteins and the non-structural Bet protein. Both the Gag precursor molecules of 70 and 74K apparent M r and the cytoplasmic 60K M r Bet protein were found to be phosphorylated, the latter being synthesized in large amounts in infected cells. Rabbit antiserum raised against recombinant human foamy virus (HFV) Gag major capsid protein cross-reacted with foamy viruses of chimpanzee, gorilla, orang-utan, rhesus monkey and African green monkey origin. This was reflected by a broad cross-reactivity of the respective monkey sera to the Gag proteins of the various foamy virus isolates. Cross-reactivity of antisera against the Bet protein was restricted to viruses from man and the great apes. Recombinant Gag and Bet proteins expressed in prokaryotes or in insect cells were readily recognized by foamy virus-positive primate sera. Screening serum samples from chimpanzees with HFV Gag and Bet proteins expressed by recombinant baculoviruses revealed that 18 out of 35 (52 %) were positive for Gag antibodies. Of these, 13 (72%) showed antibodies against the Bet protein, indicating that Bet antigen is of value in serological screening for foamy virus infections.

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Section: Discussionsupporting

confidence: 90%

Reactivity of primate sera to foamy virus Gag and Bet proteins

Hahn¹,

Baunach²,

Bräutigam³

et al. 1994

Journal of General Virology

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“…1A). The start of detectable plasma viremia, T 0, is approximately 10 days (range, 7 to 21 days) (7,11,14,21,40,52) after virus transmission and represents the end of the eclipse phase of HIV infection. The plasma samples studied from this cohort had the most frequent sampling for testing within the first 20 days before and the first 20 days after the start of detectable viremia (Fig.…”

Section: Resultsmentioning

confidence: 99%

Initial B-Cell Responses to Transmitted Human Immunodeficiency Virus Type 1: Virion-Binding Immunoglobulin M (IgM) and IgG Antibodies Followed by Plasma Anti-gp41 Antibodies with Ineffective Control of Initial Viremia

Tomaras

Yates

Liu

et al. 2008

J Virol

536

605

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“…HIV is readily found during acute (primary) infection. Titers as high as 5,000 infectious particles (IP) per ml have been reported (179,217,339,676,892). Subsequently, within weeks, the level of free virus detected in the blood is markedly reduced to sometimes <1 IP per ml (199,277,471,892,1021).…”

Section: Hiv In Body Fluids and Its Reiation To Virus Transmissionmentioning

confidence: 99%

“…Subsequently, usually within 2 weeks, IgG levels could be demonstrated by an indirect-immunofluorescence assay. Recently, by using the present antibody enzyme-linked immunosorbent assay (ELISA) detection kits, which have increased sensitivity and specificity, antibody seroconversion can be detected within a few days, although several months are required for some individuals (339,1181,1183).…”

Section: Acute Hiv Infectionmentioning

confidence: 99%