Antibody response in patients with cutaneous leishmaniasis infected by Leishmania (Viannia) braziliensis or Leishmania (Viannia) guyanensis in Brazil

Romero, Gustavo Adolfo Sierra; Orge, Maria de La Glória Orge; Guerra, Marcus Vinitius de Farias; Paes, Marcilene Gomes; Macêdo, Vanize; Carvalho, Edgar M.

doi:10.1016/j.actatropica.2004.09.005

Cited by 49 publications

(44 citation statements)

References 25 publications

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“…However, assuming that the humoral immune response may have a modulator role on the cellular response during the infection, (Bittencourt et al, 1968), suggested the possibility of researching antibodies as a way to monitor the ATL cure. The dynamics of the antibody production after treatment is still not well known, and the levels of antibodies against specific antigens remain undefined (Romero et al, 2005). The problem can be attributed to the lack of a standard antigenic preparation to detect specific antibodies.…”

Section: Introductionmentioning

confidence: 99%

Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G

Oliveira

Castro

Almeida

et al. 2013

Journal of Immunological Methods

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Section: Introductionmentioning

confidence: 99%

Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G

Oliveira

Castro

Almeida

et al. 2013

Journal of Immunological Methods

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“…Antileishmanial antibodies can be detected by serological tests; however, these are not the usual methods, because antibodies tend to be undetectable or present in low titers due to poor humoral response [21][22][23].…”

Section: Diagnosis Of Cutaneous and Mucocutaneous Leishmaniasismentioning

confidence: 99%

Diagnosis of leishmaniasis

Elmahallawy

Martínez

Rodríguez‐Grangér

et al. 2014

J Infect Dev Ctries

149

119

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Leishmaniasis is a clinically heterogeneous syndrome caused by intracellular protozoan parasites of the genus Leishmania. The clinical spectrum of leishmaniasis encompasses subclinical ( not apparent), localized (skin lesion), and disseminated (cutaneous, mucocutaneous, and visceral) infection. This spectrum of manifestations depends on the immune status of the host, on the parasite, and on immunoinflammatory responses. Visceral leishmaniasis causes high morbidity and mortality in the developing world. Reliable laboratory methods become mandatory for accurate diagnosis, especially in immunocompromised patients such as those infected with HIV. In this article, we review the current state of the diagnostic tools for leishmaniasis, especially the serological test.

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“…However, several papers which described encouraging results by the use of alternative diagnostic methods have been reported from the New World (13,16,29,32).…”

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confidence: 99%

Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused byLeishmania tropicain Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

Zeyrek

Korkmaz

Özbel

2007

Clin Vaccine Immunol

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In this study, we aimed to evaluate the validity of the conventional enzyme-linked immunosorbent assay (ELISA) and the Western blotting test for the diagnosis of anthroponotic cutaneous leishmaniasis (ACL) using serum samples obtained from 51 patients with parasitologically proven nontreated CL (NonT-CL patients) and 62 patients under treatment for CL (UT-CL patients). Additionally, 29 serum samples obtained from patients with parasitologically and serologically proven visceral leishmaniasis (VL) were also used as positive controls, and serum samples from 43 blood donors were used as negative controls. All sera were diluted to the same dilution (1/100). Leishmania infantum MON-1 was used as the antigen in the conventional ELISA. The sera of 27 (93.1%) of 29 VL patients were seropositive by ELISA, while the sera of 40 (78.4%) of 51 NonT-CL patients and 43 (69.3%) of 62 UT-CL patients were seropositive by the conventional ELISA. The absorbance values of the CL patients' sera were significantly lower than the absorbance values of the VL patients' sera. Bands between 15 and 118 kDa were detected in two groups of CL patients. Among all bands, the 63-kDa band was found to be more sensitive (88.5%). When we evaluated the Western blotting results for the presence of at least one of the diagnostic antigenic bands, the sensitivity was calculated to be 99.1%. By using serological tests, a measurable antibody response was detected in most of the CL patients in Sanliurfa, Turkey. It is also noted that this response can be changed according to the sizes, types, and numbers of lesions that the patient has. The Western blot test was found to be more sensitive and valid than the conventional ELISA for the serodiagnosis of ACL. In some instances, when it is very difficult to demonstrate the presence of parasites in the smears, immunodiagnosis can be a valuable alternative for the diagnosis of ACL.

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Antibody response in patients with cutaneous leishmaniasis infected by Leishmania (Viannia) braziliensis or Leishmania (Viannia) guyanensis in Brazil

Cited by 49 publications

References 25 publications

Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G

Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G

Diagnosis of leishmaniasis

Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused byLeishmania tropicain Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

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