Antibody Repertoire and Gene Expression Profile: Implications for Different Developmental and Functional Traits of Splenic and Peritoneal B-1 Lymphocytes

Kretschmer, Karsten; Jungebloud, Anke; Stopkowicz, Jana; Stoermann, Britta; Hoffmann, Reinhard; Weiß, Siegfried

doi:10.4049/jimmunol.171.3.1192

Cited by 30 publications

(66 citation statements)

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“…In addition, some repertoire dominating sequences could be found repeatedly in different mice [17,18]. Thus, a substantial fraction of B-1a cells expresses identical BCRs on their surface providing an excellent molecular marker for such cells.…”

Section: Introductionmentioning

confidence: 84%

Section: Gcs Are Formed In the Pps Of L2 Micementioning

confidence: 99%

“…Hence, we argued that the only unchangeable marker for a B cell is its receptor. Since it is known that the IgH repertoire of B-1 B cells comprises specificities typical for this B-cell subpopulation, the database established by us for PEC and splenic B-1-cell-associated specificities from L2 mice [17,18] could be used as marker for B-1 cells in the GALTs of such mice. This assumption turned out to be correct.…”

mentioning

confidence: 99%

“…If yes, then there should be some overlap between the repertoires of B-1 cells from these two compartments. To investigate the possible commonality, previously established PEC B-1a-cell (IgM + CD5 + )-associated IgVH sequences of L2 mice [17,18] were used as molecular markers to track the presence of such B cells in the PP of L2 mice.Using this concept, IgMVH sequences derived from bulk sorted GC B cells (CD19 + PNA Hi/Int ) from PPs of L2 mice were analyzed. Of 43 such IgMVH sequences, four were found to be identical (100% CDR3 region nucleotide sequence match) to already known (a dataset of around 800 sequences) PEC B-1a-cell-derived IgMVH sequences of L2 mice (Table 1, Sort I).…”

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B‐1‐cell subpopulations contribute differently to gut immunity

et al. 2013

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IntroductionIgA is the major class of Ab present in mucosal tissues of mammals. IgA constitutes a key defense mechanism against invasion by inhaled or ingested pathogens. IgA is also found at significant concentrations in the serum of many species, where it mediates the elimination of pathogens that have breached the mucosa [1].Among the various MALTs, IgA-producing cells are present in highest numbers in GALTs constituted by Peyer's patches (PPs), isolated lymphoid follicles (ILFs), and solitary intestinal lymphoid Correspondence: Dr. Bishnudeo Roy e-mail: Bishnudeo.Roy@helmholtz-hzi.de tissue (SILT) [2,3]. B cells, present in the B-cell follicles of such organized structures, form GCs upon Ag encounter. Under the influence of various cellular and molecular factors -T cells and cytokines, for instance -these B cells are thought to switch from IgM to IgA [4].Multiple pathways of IgA induction have been reported. The T-cell-dependent pathway of IgA induction is well known and, it is clear that IgA can also be induced in T-cell-independent (TI) manner (e.g. in T-cell-deficient mice, CD40 −/− mice, CD28 −/− mice, etc.) [5][6][7]. However, the precise contribution of T-cell-dependent and TI pathways to IgA production is not known.An important contribution of B-1 cells to TI responses has been suggested [6,8,9]. Peritoneal cavity (PEC) B-1 cells have also been claimed to contribute significantly to IgA-producing plasma cell (PC) Eur. J. Immunol. 2013Immunol. . 43: 2023Immunol. -2032. Importantly, according to phenotype, origin, and function, PEC B-1 cells can be divided into B-1a and B-1b subpopulations [12,13]. Phenotypically, B-1a cells are characterized as B220 lo CD19 hi IgM hi IgD lo CD43 + Mac-1 + CD5 int . B-1b cells share all the aforementioned markers with B-1a cells except CD5. With respect to differential contribution of these two B-1-cell subtypes to IgA production, recently, we could show that most of the IgA-secreting cells in the PEC of unmanipulated mice belonged to B-1b-cell subpopulation [14]. Additionally, IgA-derived VH regions from B-1b cells also contained frequent single nucleotide exchanges indicative of somatic hypermutation (SHM) [14]. Thus, a contribution of PEC B-1b cells to IgA production and hence to gut-associated immunity is quite likely. However, in spite of evidential suggestions for the contribution of B-1 cells to the intestinal PC pool, their contribution under unmanipulated conditions remains uncertain. This is partly due to the lack of appropriate markers to distinguish PCs according to their origin. In this context, a sequence-based approach to address this question should become very useful.In the present work, to investigate the participation of PEC B-1 cells in the gut-associated IgA production we have made use of the transgenic mouse model known as the L2 mouse line [15]. Mice of this line are characterized by the expression of a transgenic λ light chain obtained from the plasmacytoma MOPC315 (λ2 315 ). PEC of these mice contains almost exclusively CD5 + B-1a cells, whi...

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Section: Introductionmentioning

confidence: 84%

Section: Gcs Are Formed In the Pps Of L2 Micementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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B‐1‐cell subpopulations contribute differently to gut immunity

et al. 2013

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“…Unlike peritoneal B-1 cells, splenic B-1 cells do not survive well in vitro, nor do they spontaneously secrete IgM [23]. Further, in a transgenic mouse model, splenic B-1 cells were shown to differ from their peritoneal counterparts in antibody repertoire [24]. Based on this evidence, it seems reasonable to hypothesize that B-2 cells from different locations are not identical.…”

Section: Introductionmentioning

confidence: 98%

Peritoneal B‐2 cells comprise a distinct B‐2 cell population with B‐1b‐like characteristics

et al. 2006

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B‐1 and B‐2 cells are lymphocyte populations that differ in development, surface marker expression, tissue localization, and function. Though mainly found in the spleen, lymph nodes, and circulation of mice, small numbers of B‐2 cells are found in the peritoneal cavity, a site predominantly populated by B‐1 cells. Here, we characterized peritoneal B‐2 cells, and determined their relationship to B‐1 cells. We found that peritoneal B‐2 cells appear to be intermediate between splenic B‐2 and peritoneal B‐1 cells in terms of surface marker expression of B220, CD80, and CD43, expression of several marker genes, and in vitro viability and IgM secretion. Adoptive transfer of peritoneal B‐2 cells into severe combined immunodeficiency mice resulted in the acquisition of a phenotype reminiscent of B‐1b cells, as shown by up‐regulation of Mac‐1 and CD43, and down‐regulation of CD23. Moreover, adoptively transferred peritoneal B‐2 cells recapitulated B‐1 cell function by producing natural IgM in recipient mice. These data suggest that peritoneal B‐2 cells express some characteristics of B‐1b cells and that this similarity increases with additional time in the peritoneal cavity.

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Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry

Rothaeusler

Baumgarth

2006

Cytometry Pt A

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Background: Measurement of cell proliferation via BrdU incorporation in combination with multicolor cell surface staining would facilitate studies on cell subsets that require multiple markers for their identification. However, the extent to which the often harsh cell preparation procedures required affect the staining quality of more recently developed fluorescent dyes has not been assessed. Methods: Three cell preparation protocols for BrdU measurement were compared for their ability to maintain fluorescent surface staining and scatter parameters of in vivo BrdU-labeled cells by flow cytometry. A 10-color fluorescent panel was developed to test the quality of surface staining, following cell treatment and the ability to perform BrdU measurements on even small B lymphocyte subsets. Results: All cell preparation procedures affected the quality of fluorescent and/or scatter parameters to varying

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Antibody Repertoire and Gene Expression Profile: Implications for Different Developmental and Functional Traits of Splenic and Peritoneal B-1 Lymphocytes

Cited by 30 publications

References 38 publications

B‐1‐cell subpopulations contribute differently to gut immunity

B‐1‐cell subpopulations contribute differently to gut immunity

Peritoneal B‐2 cells comprise a distinct B‐2 cell population with B‐1b‐like characteristics

Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry

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