Antibody recognition of cathepsin L1-derived peptides in Fasciola hepatica-infected and/or vaccinated cattle and identification of protective linear B-cell epitopes

Abstract

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Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach

“…First, we observed a significant production of specific anti-FhCL1 IgG1 after vaccination in both vaccinated groups and also after infection in the infected-control sheep, as expected and observed in previous studies [ 12 , 17 , 20 , 26 ]. In addition, when data were compared, IgG1 level was highest throughout the trial in the vaccinated-Montanide group.…”

“…This finding aligns with those reported in a previous study conducted in rats, in which vaccination with a synthetic peptide of FhCL1 comprising the region 104–122 (sequence NRAVPDKIDWRESGYVTE, in part overlapping the region described above) resulted in a significant 40% reduction of the fluke burden [ 25 ]. On the contrary, this region was not recognised in cattle, either in partially protected animals vaccinated with FhLC1 or in vaccinated-unprotected or unvaccinated-infected cattle [ 26 ].…”

“…The study was carried out according to the protocol of Garza-Cuartero et al [ 26 ]. Briefly, a total of 160 overlapping peptides corresponding to the amino-acid sequence of FhCL1, each 9 amino acids in length with an overlap of 7 amino acids between successive peptides were synthesised (Mimotopes Pty.…”

“…With regard to members of the cysteine protease family, Harmsen et al [ 25 ] identified protective peptides within the whole F. hepatica cathepsin L1 and L3 (FhCL1 and FhCL3) capable of inducing up to 63.6% of acquired resistance to infection in rats. Likewise, regions of FhCL1 were highly recognised by vaccinated-protected cattle during a B-cell epitope mapping study [ 26 ] and, what is more, when some synthetic peptide mimotopes of the FhCL1 were used as vaccine in sheep and goats, a significant level of protection between 35–79% was achieved [ 16 , 27 ].…”

A Vaccine Based on Kunitz-Type Molecule Confers Protection Against Fasciola hepatica Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production

“…Over the last 25 years, there have been numerous attempts to formulate a successful vaccine against F. hepatica by using parasite extracts or individual antigens (14,15). These formulations achieved different levels of protection in experimental models of mice, rats, sheep, and cattle (1,16,17) by the induction of an antibody response and/or Th1/Th17-mediated cellular immunity (18)(19)(20)(21)(22).…”