Antibody probes in diagnosis and classification of lymphomas

El, Jones

doi:10.1002/path.1711410307

Cited by 1 publication

(1 citation statement)

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“…The position of non-Hodgkin's lymphoma in the differentiation pathway is less clear, as development from centroblasts to centrocytes within active lymph nodes is not solely related to the generation of antibody secreting cells.25 26 Considerable variation in surface /2m expression was found in our cases of non-Hodgkin's lymphoma, and it is considered that this reflects the immunological heterogeneity of this group.…”

Section: Discussionmentioning

confidence: 87%

Cell surface expression of beta 2-microglobulin (beta 2m) correlates with stages of differentiation in B cell tumours.

Jones¹,

Scott²,

Norfolk³

et al. 1987

J Clin Pathol

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SUMMARY Cell surface 32-microglobulin (f2m) densities of malignant B cells were determined by enzyme immunoassay in 97 cases of immunologically defined lymphoproliferative disease. Absolute f2m densities were found to depend on disease category with the lowest levels found on cells from chronic lymphocytic leukaemia (mean = 5-6 ng/106 cells, n = 27); atypical chronic lymphocytic leukaemia (mean = 5.9 ng/106 cells, n = 8); and prolymphocytoid chronic lymphocytic leukaemia variant (mean = 6 0 ng/106 cells, n = 16). fi2m densities for B non-Hodgkin's lymphoma (n = 14) and B prolymphocytic leukaemia (n = 17) cases were 8 1 and 10-0 ng/106 cells, respectively, and the highest densities were found on cells from "late-B cell" tumours (mean = 14 3 ng/106 cells). Plasma cells from cases of Ig secreting tumours expressed unexpectedly low fJ2m densities (mean = 9.3 ng/106 cells; n = 6).f12-microglobulin (fl2m) forms the invariant light chain of the HLA class I molecule' and is expressed, in non-covalent association with the 43000 dalton heavy chain,2 on the surface of cells from many tissues.34 HLA antigens seem to play a part in regulating the immune response by major histocompatibility complex restriction of T cell mediated cytotoxicity,5 and f2m is required for the structural integrity of the HLA molecule.6`8 Defective expression of HLA has been reported in a variety of tumours,49 and it is possible that this impairs T cell mediated responses to tumour cells.'0 fi #2m is shed into plasma as a result of normal membrane turnover'2 and is raised in many pathological conditions."3 Correlations between serum 152m and estimated tumour mass have been noted in chronic lymphocytic leukaemia"4 and myeloma,'5 and serum #2m is also closely correlated with prognosis in myeloma.'6 The underlying importance of these observations has yet to be determined. Further insights may be gained by examining relations between serum concentration and cell surface expression. We present the findings of a vertical study into surface 132m expression in malignant lymphoid cells from patients with morphologically and immunologically classified B cell disorders.

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Section: Discussionmentioning

confidence: 87%