Antibody Printing Technologies

Optimization of an Antibody Microarray Printing Process Using a Designed Experiment

“… 1 − 7 They have contributed significantly to “life-omics”, as well as disease diagnostics and management. 8 In general, antibody arrays are fabricated by immobilizing multiple antibodies on a solid substrate, such as a chemically treated glass slide or well plate, or a nitrocellulose (NC) paper membrane, to analyze antibody–antigen interactions. 9 Due to the assay cost associated with a large number of antibodies, microarray fabrication emerged to miniaturize the arrays with thousands of spots within centimeters.…”

“…Different technologies have been utilized to print antibody arrays, including contact printing (pin, stamp, stencil, and microfluidic printings), noncontact printing (inkjet printing), lithography printing (photolithography, electron beam lithography), and atomic force microscopy (AFM) printing (dip-pen lithography and nanoshaving). 8 Each printing technology has their own merits and drawbacks. However, current commercial microarray printing systems mainly focus on two printing technologies, i.e., pin contact printing and inkjet noncontact printing.…”

“…Antibody microarrays are currently being used for numerous applications including analysis of proteins, nucleic acids, cell surface proteins, and simultaneous detection of these analytes. − They have contributed significantly to “life-omics”, as well as disease diagnostics and management . In general, antibody arrays are fabricated by immobilizing multiple antibodies on a solid substrate, such as a chemically treated glass slide or well plate, or a nitrocellulose (NC) paper membrane, to analyze antibody–antigen interactions .…”