Antibody Phage Display

Nur, Alia; Schubert, Maren; Lai, Jing Yi; Hust, Michael; Choong, Yee Siew; Isa, Wan Yus Haniff Wan; Lim, Theam Soon

doi:10.1007/978-1-0716-3381-6_1

Cited by 1 publication

(2 citation statements)

References 53 publications

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“…A plausible alternative that allows for faster production of mAbs is through in vitrodirected protein evolution procedures, which involve (1) the generation of native and immune gene libraries [12] or synthetic [13] that encode single-chain variable fragment (scFv) mAbs displayed through phage (phage display) [14]; (2) the process of screening and selection of isoforms related to an immobilized antigen (target proteins and/or previously identified biomarkers) [15]; and (3) the in vitro maturation of the affinity or specificity of the antibody, through the insertion of random mutations and selection of the isoform with improved affinity [16].…”

Section: Introductionmentioning

confidence: 99%

“…Phage display technology enables the expression and selection of high-affinity recombinant antibodies in laboratories in a controlled manner. These antibodies are fused with proteins from the phage coat and displayed on its surface, facilitating the isolation of scFv and Fab fragments targeting specific cellular markers [14]. Antibody libraries can be generated from pre-immune donors using V domains from the germ line or synthetic genes [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Construction of a Human Immune Library from Gallbladder Cancer Patients for the Single-Chain Fragment Variable (scFv) Antibody Selection against Claudin 18.2 via Phage Display

Effer,

Ulloa,

Dappolonnio

et al. 2024

Antibodies

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Gallbladder cancer (GBC) is a very aggressive malignant neoplasm of the biliary tract with a poor prognosis. There are no specific therapies for the treatment of GBC or early diagnosis tools; for this reason, the development of strategies and technologies that facilitate or allow an early diagnosis of GBC continues to be decisive. Phage display is a robust technique used for the production of monoclonal antibodies (mAbs) involving (1) the generation of gene libraries, (2) the screening and selection of isoforms related to an immobilized antigen, and (3) the in vitro maturation of the affinity of the antibody for the antigen. This research aimed to construct a human immune library from PBMCs of GBC patients and the isolation of scFv-phage clones with specificity against the larger extracellular loop belonging to claudin 18.2, which is an important biomarker overexpressed in GBC as well as gastric cancer. The immune-library-denominated GALLBLA1 was constructed from seven GBC patients and has a diversity of 6.12 × 1010 pfu mL−1. After three rounds of panning, we were able to identify clones with specificity against claudin 18.2. GALLBLA1 can contribute to the selection, isolation, and recombinant production of new human mAbs candidates for the treatment of gastrointestinal cancers.

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