Antibody-mediated resistance against plant pathogens

Safarnejad, Mohammad Reza; Jouzani, Gholamreza Salehi; Tabatabaie, Meisam; Twyman, Richard M.; Schillberg, Stefan

doi:10.1016/j.biotechadv.2011.08.011

Cited by 47 publications

(30 citation statements)

References 135 publications

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“…Phage display, involves the introduction of peptide sequences (such as the antigen-binding domains of recombinant antibodies) into the coat protein gene of a bacteriophage displayed on the virion surface. Phage antibody display libraries can be screened and the corresponding antibody gene can then be recovered from the phage genome (40). This technique is simple, cheap, and rapid and requires no special equipment.…”

Section: Shahryari F Et Al Preparation Of Antibody Against Imp Of Camentioning

confidence: 99%

Preparation of Antibody Against Immunodominant Membrane Protein (IMP) of Candidatus Phytoplasma aurantifolia

Shahryari¹,

Shams-Bakhsh²,

Safarnejad³

et al. 2013

Iran J Biotech

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Background:The witches' broom disease of lime caused by Candidatus Phytoplasma aurantifolia, is the most devastating disease of acidian lime in the southern parts of Iran. Objectives: At present, no efficient method has been developed for controlling the disease, therefore quarantine approaches such as early detection and subsequent eradication of infected trees is very important. Toward this aim, developing a reliable and sensitive detection method would be the first step to prevent transportation of infected plant materials to other places. Materials and Methods: In this study, Immunodominant membrane protein (IMP) of the pathogen was selected as a target for detection and preparation of polyclonal antibody. The IMP is the major protein present on the surface of phytoplasma cells. For this purpose, the DNA region encoding IMP gene was isolated and cloned into pET28a bacterial expression vector. The recombinant protein was expressed in a large scale in Escherichia coli. Purification was performed under native conditions and the purity and integrity of produced recombinant protein were confirmed by western immuno blot analysis using anti His-tag and anti-IMP polyclonal antibodies. The purified recombinant IMP was used for immunization of rabbit. Purification of immunoglobulin was performed by affinity chromatography using protein A column. The purified immunoglobulin was conjugated with the alkaline phosphatase enzyme. Results: The purified antibodies and conjugates were applied for efficient detection of infected plants in double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and dot immunosorbent assay (DIBA). Conclusions: These antibodies were proven to be very powerful tools to detect the Candidatus Phytoplasma aurantifolia in plants.

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Section: Shahryari F Et Al Preparation Of Antibody Against Imp Of Camentioning

confidence: 99%

Preparation of Antibody Against Immunodominant Membrane Protein (IMP) of Candidatus Phytoplasma aurantifolia

Shahryari¹,

Shams-Bakhsh²,

Safarnejad³

et al. 2013

Iran J Biotech

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“…Scale bar, 25 lm in all images activity [19,20]. Although many recombinant antibodies have been expressed successfully in transgenic plants [21,22], they have generally been directed to different subcellular compartments [23][24][25] because the cytoplasm does not provide a favorable environment for protein folding and the formation of disulfide bonds [26]. Functional scFvs have been expressed in the cytoplasm of Escherichia coli [27], but only a small number have proven to be stable and functional in the plant cytoplasm [26,28,29].…”

Section: Discussionmentioning

confidence: 99%

In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae)

Zakri

Ziegler²,

Commandeur

et al. 2012

Arch Virol

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“…In the phage display A B libraries, billions of peptides capable binding to different antigens are present on the surface of the bacteriophages. Screening and panning techniques will recover the specific antibody gene [22]. This technique is simple, cheap, and rapid and requires no special equipment.…”

Section: Discussionmentioning

confidence: 99%

Production of a phage-displayed single chain variable fragment antibody against infectious bursal disease virus

Pashaki¹,

Safarnejad²,

Safdar³

et al. 2018

Trop. J. Pharm Res

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Antibody-mediated resistance against plant pathogens

Cited by 47 publications

References 135 publications

Preparation of Antibody Against Immunodominant Membrane Protein (IMP) of Candidatus Phytoplasma aurantifolia

Preparation of Antibody Against Immunodominant Membrane Protein (IMP) of Candidatus Phytoplasma aurantifolia

In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae)

Production of a phage-displayed single chain variable fragment antibody against infectious bursal disease virus

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