Antibody-Mediated Neutralization of Pertussis Toxin-Induced Mitogenicity of Human Peripheral Blood Mononuclear Cells

Abstract

Antibody-mediated neutralization of pertussis toxin-induced proliferation of human peripheral blood mononuclear cells (PBMC) was assessed using alamarBlue and compared with results from the Chinese hamster ovary (CHO) cell assay using sera from vaccinated adults and convalescent children. Neutralization values for the CHO assay were similar for vaccinated and convalescent subjects; however. the convalescent group had higher titers in the PBMC assay. Results for pertussis toxin neutralization with the CHO assay appear to be distinct from those with the PBMC assay.

A Framework for Assessing Immunological Correlates of Protection in Vaccine Trials

“…Several pediatric studies of acellular pertussis vaccines have identified CoRs for pertussis disease, including postvaccination levels of antibody to pertactin, fimbriae, pertussis toxin, and filamentous hemagglutinin [28,29]. Almost all investigations of immune correlates reported in the literature have been done at the CoR level, and there is much uncertainty about the value of the different serological measurements as SoPs [30]. As an exception, Storsaeter et al [28] applied regression models that supported anti-pertactin, anti-fimbriae, and anti-pertussis toxin antibody levels as a joint level 1 SoP S .…”

Effect of glycounits on the antimicrobial properties and toxicity behavior of polymers based on quaternized DMAEMA

“…Therefore, the appropriate antimicrobial polymer will depend on the final application the polymer. For instance, S6Q (i.e., PBMA 132 -b-P(DMAEMAQ 0.88 -co-HEMAGl 0.12 ) 127 ) and T1 (i.e., PBMA 132 -b-PDMAEMAQ 45 -b-PHEMAGl 6 ) would be very promising materials against Gram-positive bacteria and fungi in coatings and paints because their demonstrated high …”

Acellular Pertussis Vaccines and Complement Killing ofBordetella pertussis

“…IgG and IgA titers to vaccine antigens. An ELISA was used to measure antibodies to pertussis toxin, FHA, and pertactin as previously described (16). All of the subjects receiving the acellular pertussis vaccine had increased IgG titers to each of the three vaccine antigens after immunization (Table 1), and many individuals had increased IgA titers.…”