Antibody mediated cotton-archetypal substrate for enumeration of circulating tumor cells and chemotherapy outcome in 3D tumors

Arora, Smriti; D’Souza, Alain; Singh, Balram; Aland, Gourishankar; Kale, Narendra; Jadhav, Bhagwat; Kad, Trupti; Chaturvedi, Pankaj; Khandare, Jayant

doi:10.1039/d2lc00004k

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Ex Vivo Culture and Testing Of Drug Susceptibility1

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2023

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Cited by 2 publications

(1 citation statement)

References 35 publications

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“…11D). 164 CMS with nanostructured surface can effectively isolate CTCs and act as a matrix to promote the formation of 3D tumor spheres in capturing cells, and its size increases by 5 times from the 3rd day to the 10th day of culture. In addition, when treated with the anticancer drug cisplatin, the tumor size was reduced by almost 1 to 2 within 24 hours.…”

Section: Ex Vivo Culture and Testing Of Drug Susceptibilitymentioning

confidence: 99%

Recent progress of nanostructure-based enrichment of circulating tumor cells and downstream analysis

Liu

Cheng

et al. 2023

Lab Chip

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Section: Ex Vivo Culture and Testing Of Drug Susceptibilitymentioning

confidence: 99%

Recent progress of nanostructure-based enrichment of circulating tumor cells and downstream analysis

Liu

Cheng

et al. 2023

Lab Chip

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Polyglycerol‐Based Biomedical Matrix for Immunomagnetic Circulating Tumor Cell Isolation and Their Expansion into Tumor Spheroids for Drug Screening

Tang

Thongrom

Arora

et al. 2023

Adv Healthcare Materials

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Circulating tumor cells (CTCs) are established as distinct cancer biomarkers for diagnosis, as preclinical models, and therapeutic targets. Their use as preclinical models is limited owing to low purity after isolation and the lack of effective techniques to create 3D cultures that accurately mimic in vivo conditions. Herein, a two‐component system for detecting, isolating, and expanding CTCs to generate multicellular tumor spheroids that mimic the physiology and microenvironment of the diseased organ is proposed. First, an antifouling biointerface on magnetic beads is fabricated by adding a bioinert polymer layer and conjugation of biospecific ligands to isolate cancer cells, dramatically enhancing the selectivity and purity of the isolated cancer cells. Next, the isolated cells are encapsulated into self‐degradable hydrogels synthesized using a thiol‐click approach. The hydrogels are mechanochemically tuned to enable tumor spheroid growth to a size greater than 300 µm and to further release the grown spheroids while retaining their tumor‐like characteristics. In addition, drug treatment highlights the need for 3D culture environments rather than conventional 2D culture. The designed biomedical matrix shows potential as a universal method to ensure mimicry of in vivo tumor characteristics in individual patients and to improve the predictability of preclinical screening of personalized therapeutics.

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Antibody mediated cotton-archetypal substrate for enumeration of circulating tumor cells and chemotherapy outcome in 3D tumors

Abstract: Circulating tumor cells (CTCs) represent a distinct cancer biomarker established in clinical settings for early cancer detection, metastasis progression, and minimal residual disease (MRD) monitoring. Despite numerous advances, the comprehensive...

Cited by 2 publications

References 35 publications

Recent progress of nanostructure-based enrichment of circulating tumor cells and downstream analysis

Recent progress of nanostructure-based enrichment of circulating tumor cells and downstream analysis

Polyglycerol‐Based Biomedical Matrix for Immunomagnetic Circulating Tumor Cell Isolation and Their Expansion into Tumor Spheroids for Drug Screening

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