Antibody-labeled Gold Nanoparticles Based Immunosensor for the Detection of Thyroxine Hormone

Abstract

Immunosensors are used to provide simple, quick and effective ways to detect analytes for the diagnosis of a number of diseases. This work reports on a novel optical immunosensor based on thyroxine antibody labeled gold nanoparticles (anti-T4/C-AuNPs) for the sensitive detection of the thyroxine (T4) hormone. T4 hormone is a reliable diagnostic biomarker present in the human blood serum that is necessary for normal neural development and cellular metabolism. The free T4 hormone, a major key player of the total biologically active T4 hormone, in serum regulates the patient's thyroid function. The immunosensor was synthesized by using amine functionalized cysteamine capped gold nanoparticles (C-AuNPs) of size 36 nm approximately. The T4 specific antibody (anti-T4) was covalently attached to C-AuNP by EDC-NHS (1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide) cross-linking chemistry. This anti-T4/C-AuNP bioconjugate was used for the analysis of the free thyroxine hormone in samples. The proposed method exhibited high performance immunosensors having a linear range of 0.52 to 65.1 pg/mL and the detection limit of 9.11 pg/mL.

Topology-Dependent Interaction of Cyclic Poly(ethylene glycol) Complexed with Gold Nanoparticles against Bovine Serum Albumin for a Colorimetric Change

“…Aggregation of AuNPs often causes a change in the color due to the surface plasmon resonance coupling. However, fainting in the red color without a shift in the absorption wavelength was reported in thyroxine hormone detection by antibody-labeled AuNPs . This was likely caused by the sufficient separation of each AuNP by the antibody and antigen even in the aggregates.…”

Recent research trends in voltammetric sensing platforms for hormones and their applications to human serum analyses

“…[25][26][27][28][29][30] Some of the hormones, including DA, EP, NE, and 5-HT are also recognized as neurotransmitters, whereas T3 and T4 are thyroid hormones that were closely related to thyroid function. 31 In this section, some recently reported examples of representative voltammetric sensing platforms and their applications in human serum sample analyses are discussed, as summarized in Table 1. Both direct oxidation and biorecognition elements involved detection strategies were used with various nanomaterials to improve the sensitivity via helping electron transfer rate and electrocatalytic activity, as well as enriching the surface active sites.…”

Dual colorimetric sensing of ascorbic acid and thyroxine using Ag–EGCG–CTAB via a DFT approach

“…13 Antibody-labeled AuNP immune-sensors can sense TH up to 9.11 pg ml À1 . 17 Herein, we report a simple, green, cost-effective and timesaving colorimetric method for sensing both AA and TH using AgNPs synthesized from Elsholtzia blanda Benth. ower (EBF) capped with CTAB (cetyltrimethylammonium bromide) (Ag-EGCG-CTAB).…”