Antibody engineering: Molecular constructor on the basis of barnase-barstar module

Abstract

Today, antibody engineering for clinical applications is a rapidly progressing field of science and a big business. The basic functions of an antibody can be spatially differentiated and attributed to various structural domains of a molecule. Therefore, each of them may be an object for engineering with the aim of using a definite antibody function. In this sense, the potential of antibodies is unique. In this article, recent achievements and current problems of antibody engineering are briefly reviewed. The main attention is focused on a molecular constructor that allows for obtaining, with the help of a versatile barnase-barstar module, mono- and multiva-lent miniantibodies and their derivatives with outlined properties.

Post-Assay Chemical Enhancement for Highly Sensitive Lateral Flow Immunoassays: A Critical Review

“…As the binding between nanoparticles is polyvalent (i.e., multiple molecules crosslink nanoparticles), moderate/low affine bindings can be used, as the high stability (i.e., no dissociation of nanoparticles aggregates) will be determined by high avidity [ 117 ]. Post-assay crosslinking can be driven by boronate affinity (phenylboronic acid and its derivatives binding with cis-diol-containing molecules such as glycoproteins, glycans) [ 118 ], hybridization of nucleic acids [ 119 ], vancomycin-D-alanyl-D-alanyl fragment in Gram-positive bacterial cell wall [ 120 ], barnase–barstar [ 121 ], small molecule–protein [ 122 ] and other types of binding.…”

Supramolecular agents for theranostics

“…1b; table), and for some other biomedi cal purposes [36,37]. The application of ASBB for self assembly of more complex supramolecular structures with desired prop erties from a ready to use set of modules, which include, in addition to proteins, nanoparticles of dif ferent nature, required a fundamental study of this process.…”