Antibody Array-Generated Cytokine Profiles of Tears of Patients with Vernal Keratoconjunctivitis or Giant Papillary Conjunctivitis

Shoji, Jun; Inada, Noriko; Sawa, Mitsuru

doi:10.1007/s10384-005-0319-4

Cited by 57 publications

(32 citation statements)

References 28 publications

(25 reference statements)

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“…[1][2][3]10,19,22,[26][27][28][29]31,33,34 The cell traffic (migration) can be realized not only by means of attraction mechanisms, governed by attraction and/or chemotactic factors and adhesion molecules, but also through the so-called 'homing' mechanism of the B-and T-lymphocytes, being controlled by a number of homing factors. 1,10,22,26,27,31,32,[35][36][37][38] The disturbed homing mechanisms can then lead to the migration of the particular cell types to different localities.…”

Section: Discussionmentioning

confidence: 99%

The possible involvement of nasal allergy in allergic keratoconjunctivitis

Pelikán¹

2008

Eye

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Purpose Allergic keratoconjunctivitis coexists regularly with allergic rhinitis. However, little is known about the relationship between these conditions. The purpose of this study was to investigate the possible involvement of nasal allergy in keratoconjunctivitis by means of nasal challenge with allergen (NPT), in combination with recording of the ocular symptoms. Methods In 26 patients suffering from atopic (n ¼ 15) or vernal (n ¼ 11) keratoconjunctivitis showing positive history and skin tests, but responding insufficiently to the local ophthalmologic therapy, 71 NPTs with inhalant allergens were performed and combined with the recording of the ocular response. In 11 control subjects with allergic rhinitis, but without ocular disease history, 11 positive NPTs were repeated and supplemented with the registration of the ocular features. Results Of the 26 patients, 24 developed 51 positive nasal responses (NRs; Po0.01), 43 of which were accompanied by significant ocular response (Po0.01). No ocular responses were measured during the 26 PBS control challenges (P40.05) or during 11 repeated NPTs in control subjects (P40.2). Conclusions These results give evidence for possible involvement of nasal allergy in some cases of keratoconjunctivitis. They also show diagnostic value of nasal challenges with allergen in combination with registration of the ocular symptoms in such patients, allowing then consideration of additional therapeutic measures concerning the nasal allergy.

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Section: Discussionmentioning

confidence: 99%

The possible involvement of nasal allergy in allergic keratoconjunctivitis

Pelikán¹

2008

Eye

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“…Analysis of chemokine profi les in tears of patients with VKC has shown that eotaxin-1 and -2 are critical chemokines in the pathogenesis of VKC. 15,23 Our current results showed that detection of both eotaxin-1 and eotaxin-2 in tears may indicate an advanced stage of VKC. The concentration of eotaxin-2 in tears is thus a suitable scale biomarker for the allergic infl ammatory intensity in VKC patients.…”

Section: Discussionmentioning

confidence: 87%

“…14 In our previous antibody-array analysis study of cytokine and chemokine profi les in tears of patients with VKC and giant papillary conjunctivitis (GPC), eotaxin-2 and soluble interleukin-6 receptor increased signifi cantly in patients with VKC or GPC compared with in healthy individuals. 15 However, the biological role of the eotaxin subfamily in the pathogenesis of VKC is not fully understood. Furthermore, a recent report has suggested that conjunctival epithelial cells are capable of actively participating in immune reactions via the production of cytokines and chemokines such as interleukin (IL)-6, IL-8, and eotaxin.…”

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confidence: 99%

Evaluation of eotaxin-1, -2, and -3 protein production and messenger RNA expression in patients with vernal keratoconjunctivitis

2009

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“…Median values are presented with the range in parentheses. 1 Mann-Whitney U test. n-fold change = value of mRNA in AKC-iCALT/value of mRNA in AKC-conjunctiva.…”

Section: Discussionmentioning

confidence: 99%

“…Besides the proliferative conjunctival lesions (e.g. giant papillae and limbal gelatinous infiltration), the clinical characteristics of patients with AKC or VKC include high serum levels of antigen-specific IgE antibodies, increased expression and secretion of inflammatory cytokines (IL-4, IL-5), soluble cytokine receptors (soluble IL-6 receptor), and chemokines (eotaxin, IL-8) in tears [1][2][3][4][5][6] . In particular, high levels of these cytokines and chemokines in the tears of patients with severe ACD are thought to originate from the conjunctival epithelium and lacrimal gland, and the mast cells, eosinophils and type 2 helper T (Th2) cells in conjunctival tissue, and play a critical role in the pathogenesis of allergic inflammation.…”

Section: Introductionmentioning

confidence: 99%

Involvement of Chemokines and a CD4-Positive T Cell Subset in the Development of Conjunctival Secondary Lymphoid Follicles in an Atopic Keratoconjunctivitis Mouse Model

Shoji

Nakanishi

Ishimori

et al. 2015

Int Arch Allergy Immunol

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Background: Massive B cell lymphoid hyperplasia and its associated factors may play a role in exacerbating inflammation in allergic disorders. We here investigated the chemokines and CD4-positive T cell subset involved in the development of secondary lymphoid follicles (iCALT) in conjunctival tissues in an atopic keratoconjunctivitis mouse model (AKC mouse). Methods: NC/Nga mice were divided into three groups: AKC (percutaneous sensitization and instillation of crude house dust mite antigen), AD (percutaneous sensitization only) and C (untreated control). Pathological changes in the conjunctival tissues of each group were investigated using histological and immunohistochemical detection of CD4 and CD20. Furthermore, tissue sections of iCALT (AKC-iCALT subgroup) and conjunctiva without iCALT (AKC-conjunctiva subgroup) were obtained from AKC mice using laser-assisted microdissection. mRNA expression of chemokine and T cell subset-related transcription factors were compared between the AKC-iCALT and AKC-conjunctiva subgroups using polymerase chain reaction (PCR) array and real-time reverse transcription-PCR (RT-PCR) methods. Results: iCALT with central aggregation of CD20-positive B cells and CD4-positive T cell infiltration surrounding B cells was observed in the palpebral conjunctival tissue of the AKC group, but not in that of the AD and C groups. Chemokine and T cell subset-related transcription factor expression was confirmed using real-time RT-PCR, with significant increases in Ccl5, Ccl17, Cxl20, Cxcl3, Ccr7, Foxp3 and T-bet mRNA expression in the AKC-iCALT subgroup compared with those in the AKC-conjunctiva subgroup. Conclusions: We concluded that CCL5, CCL17 and CCL20, as well as T-bet- and Foxp3-positive lymphocytes may be iCALT-related factors and that iCALT-related chemokines are worth evaluating as biomarkers.

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Antibody Array-Generated Cytokine Profiles of Tears of Patients with Vernal Keratoconjunctivitis or Giant Papillary Conjunctivitis

Cited by 57 publications

References 28 publications

The possible involvement of nasal allergy in allergic keratoconjunctivitis

The possible involvement of nasal allergy in allergic keratoconjunctivitis

Evaluation of eotaxin-1, -2, and -3 protein production and messenger RNA expression in patients with vernal keratoconjunctivitis

Involvement of Chemokines and a CD4-Positive T Cell Subset in the Development of Conjunctival Secondary Lymphoid Follicles in an Atopic Keratoconjunctivitis Mouse Model

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