Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis

Keino, Hiroomi; Kawarada, Yoshihiko

doi:10.1007/bf00315834

Cited by 118 publications

(74 citation statements)

References 17 publications

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“…The thymus is known to be atrophied by CYadministration due to apoptosis of thymocytes. [40][41][42] Hiramine et al 41) showed a marked decrease in the proportion of CD4 ϩ…”

Section: Discussionmentioning

confidence: 99%

Effect of SCG, 1,3-β-D-Glucan from Sparassis crispa on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice

Harada

Miura

Adachi

et al. 2002

Biological & Pharmaceutical Bulletin

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The major side-effect of most anti-cancer chemotherapeutic drugs is neutropenia, and the administration of these drugs impairs blood-forming functions (e.g. the generation of neutrophils, NK cells, etc.) that are important to maintain the defense systems of the patients. As a result, chemotherapy may accelerate the risk of tumor metastases and fungal infection. Metastasis of tumor cells is a serious concern, causing deterioration in the condition of patients receiving cancer therapy.

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“…The thymus is known to be atrophied by CYadministration due to apoptosis of thymocytes. [40][41][42] Hiramine et al 41) showed a marked decrease in the proportion of CD4 ϩ…”

Section: Discussionmentioning

confidence: 99%

Effect of SCG, 1,3-β-D-Glucan from Sparassis crispa on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice

Harada

Miura

Adachi

et al. 2002

Biological & Pharmaceutical Bulletin

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“…To detect specific apoptotic cells, we used polyclonal rabbit anti-ssDNA Ab (DAKO Japan Co., Ltd., Japan). The ssDNA for producing polyclonal antibody was obtained from the double-strand calf thymus (20,32). Immunohistochemical studies with this Ab may be more specific and sensitive (23) in detecting apoptosis.…”

Section: Study Design Of Administration With Lps or Lps Plus Fts In Mmentioning

confidence: 99%

Serum Thymic Factor Prevents LPS‐Induced Pancreatic Cell Damage in Mice via Up‐Regulation of Bcl‐2 Expression in Pancreas

Saitoh

Awaya

Sakudo

et al. 2004

Microbiology and Immunology

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The protective effect of synthetic serum thymic factor (FTS) nonapeptide on lipopolysaccharide (LPS)‐induced pancreatic cell damage in 10‐week‐old BALB/c male mice was investigated. Mice were divided into three groups. Group I was treated with LPS (10 μg/head; i.p.) (LPS‐treated mice). Group II was administered with FTS (50 μg/head; i.p.) 24 hr before treatment with LPS and complemented immediately before LPS injection with FTS (50 μg/head; i.p.) (FTS‐administered mice). Group III was only treated with the same volume of saline (control mice). Treatment of LPS in vivo resulted in the destruction of pancreatic acinar cells. In those cells, many apoptotic cells were detected by immunohistochemistry using an anti‐single stranded DNA antibody. Immunohistochemistry and reverse transcription‐polymerase chain reaction (RT‐PCR) revealed that LPS treatment also caused low or a lack of insulin expression in pancreatic islets. In contrast, morphological change was not seen and apoptotic cell death was suppressed in pancreatic cells of FTS‐administered mice. Moreover, insulin expression was normal in those mice. FTS administration enhanced expression of Bcl‐2 mRNA levels in pancreatic tissues and IL‐6 mRNA levels in splenocytes significantly compared with those of LPS treatment at 3 hr after LPS injection. These findings suggest that FTS prevents LPS‐induced cell damage via enhancing Bcl‐2 expression in the pancreas and systemic IL‐6 production.

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“…27 Single-stranded DNA is a marker for increased DNA damage and apoptosis in cells exposed to injury or stress by inflammation, environmental or carcinogenic pollutants. 28,29 Recent evidence indicates that coordinated expression of several DNA repair enzymes is needed 30,31 for oxidatively generated DNA damage including modified purine and pyrimidine bases, abasic sites, 32,33 and DNA strand breaks. 34 NO is a small messenger molecule that was first discovered as a potent vasodilator, known as the endothelium derived relaxing factor, produced and released by vascular endothelial cells.…”

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confidence: 99%

Elevated oxidative stress and DNA damage and repair levels in urinary bladder carcinomas associated with schistosomiasis

Salim

Morimura

Menesi

et al. 2008

Intl Journal of Cancer

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To cast light on mechanisms underlying development of urothelial carcinomas (UCs) of the urinary bladder associated with Schistosomiasis, we immunohistochemically analyzed the relationship between oxidative stress markers, DNA single strand breaks (ssDNA) which could also measure the levels of base damage and apoptosis in DNA, and expression of DNA repair genes with levels of nitric oxide synthases in bladder carcinomas of Egyptian patients with or without Schistosoma hematobium infection. Marked elevation of 8-hydroxy-2 0 -deoxyguanosine (8-OHdG) levels was found in squamous cell carcinomas and UCs associated with Schistosomiasis when compared with non-Schistosomal carcinomas. This was accompanied by strong over expression of the DNA-repair genes, 8-oxoguanine-DNA-glycosylase and apurinic/ apyrimidinic endonuclease, as well as increased formation levels of ssDNA. Expression levels of inducible nitric oxide synthase (iNOS) which is known to be indirectly related to oxidative stress was higher in Schistosomal than in the non-Schistosomal carcinomas. However, expression of endothelial nitric oxide synthase was slightly stronger in non-Schistosomal than in the Schistosomal carcinomas. In conclusion, these findings suggest a strong correlation between Schistosoma haematobium infection and increased levels of oxidative stress accompanied by a continuous DNA damage and repair in UCs, all directly correlating with elevated iNOS. ' 2008 Wiley-Liss, Inc.Key words: oxidative stress; 8-OHdG; ROS; iNOS; eNOS; ssDNA; OGG1; APE-1; DNA repair; urinary bladder carcinoma; schistosomes Schistosomiasis (Bilharzia) is endemic in Egypt and also in other 75 countries worldwide, affecting more than 200 million people, and putting more than 1 billion population at great risk. 1 It has been well-documented that urinary bladder cancer is one of the major consequences of chronic infection with Schistosoma hematobium in many African and Middle East countries. 2 Among different species of Schistosoma inhibiting various countries and causing several diseases including cancers of the liver and colorectum, 3,4 the relationship between Schistosoma hematobium and urinary bladder cancer is the most important, and this organism has been classified by WHO/IARC as a Class 1 carcinogen. 5 When compared with their non-Schistosomal counterparts, Schistosoma-associated bladder carcinomas have 2 major clinicopathological features: the incidence reaches a peak in the 3rd-5th decades of life vs. the 7th decade in nonendemic areas, and there is significant increase in the ratios of squamous cell carcinomas (SCCs) to typical transitional urothelial carcinomas (UCs). 6 The SCCs associated with Schistosoma are extremely complex and heterogeneous, exhibiting a high level of resistance to radiotherapy and chemotherapy. However, they do not show distant metastasis in spite of their invasive characteristics and advanced clinical grades. Comparisons have already been made of SCCs and UCs of non-Schistosoma-related 7 and Schistosoma-associated origins, 8 but the fo...

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Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis

Cited by 118 publications

References 17 publications

Effect of SCG, 1,3-β-<small>D</small>-Glucan from <i>Sparassis crispa</i> on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice

Effect of SCG, 1,3-β-<small>D</small>-Glucan from <i>Sparassis crispa</i> on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice

Serum Thymic Factor Prevents LPS‐Induced Pancreatic Cell Damage in Mice via Up‐Regulation of Bcl‐2 Expression in Pancreas

Elevated oxidative stress and DNA damage and repair levels in urinary bladder carcinomas associated with schistosomiasis

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Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis

Cited by 118 publications

References 17 publications

Effect of SCG, 1,3-&beta;-<small>D</small>-Glucan from <i>Sparassis crispa</i> on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice

Effect of SCG, 1,3-&beta;-<small>D</small>-Glucan from <i>Sparassis crispa</i> on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice

Serum Thymic Factor Prevents LPS‐Induced Pancreatic Cell Damage in Mice via Up‐Regulation of Bcl‐2 Expression in Pancreas

Elevated oxidative stress and DNA damage and repair levels in urinary bladder carcinomas associated with schistosomiasis

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Effect of SCG, 1,3-β-<small>D</small>-Glucan from <i>Sparassis crispa</i> on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice

Effect of SCG, 1,3-β-<small>D</small>-Glucan from <i>Sparassis crispa</i> on the Hematopoietic Response in Cyclophosphamide Induced Leukopenic Mice