Antibody against mutated citrullinated vimentin: a new sensitive marker in the diagnosis of rheumatoid arthritis

Abstract

The aim of this study was to determine the performance of antibodies against mutated citrullinated vimentin (anti-MCV) in comparison with antibodies to cyclic citrullinated peptides (anti-CCP) in patients with rheumatoid arthritis (RA). Serum levels of anti-MCV and anti-CCP were determined in 193 patients with RA and 332 controls, and sensitivity and specificity were calculated. In a separate analysis of 86 patients, the anti-MCV levels were compared to disease activity. Sensitivity of anti-MCV versus anti-CCP was 71.5 and 69.4%, specificity was 81.3 and 97.6%, respectively. The ROC curves showed higher specificity and an advantage of anti-CCP. In seronegative RA patients the sensitivity of anti-MCV was superior over anti-CCP. Anti-MCV positivities also occurred in systemic lupus erythematosus and Sjoegren's syndrome. In a subgroup of 86 RA patients we found a significant correlation between anti-MCV and disease activity. Anti-MCV appears to be an important marker for the diagnosis of RA, and correlates also with disease activity.

Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits

“…[6,7,10,11,14,16] The results of the present study also support this outcome for the anti-MCV-548 kit. However, regarding the anti-MCV-248 kit, there was no statistically significant difference in sensitivity and specificity between anti-CCP-2 and anti-MCV antibodies.…”

“…[7,10] Wagner et al [7] found anti-MCV positivity to be 13.6% (n=8) in anti-CCP negative patients (59 patients). In a study by Nicaise-Roland et al, [10] anti-MCV was found to be positive in 18.4% (n=14) of anti-CCP negative patients (n=76).…”

“…There are many studies which have investigated the value of the anti-MCV antibodies in connective tissue diseases. [6][7][8] A meta-analysis reported the sensitivity and specificity of anti-MCV antibody as between 0.64-0.84, and 0.79-0.96 respectively, which are almost equal to those of anti-CCP in RA. [8] However, studies comparing different commercial kits are missing in the literature, particularly for anti-MCV antibodies.…”

“…There was no significant difference between patients with low and moderate disease activity in terms of RF, anti-CCP-2, anti-MCV-548 and anti-MCV-248 antibodies. There are studies suggesting a significant correlation between antibodies and disease activity [7,10,12,15,19] while others did not find any correlation. [13,17] In our study, anti-MCV-548 and anti-MCV-248 were strongly correlated with anti-CCP-2 but weakly correlated with RF.…”

Unique Correlation Between Mutated Citrullinated Vimentine IgG Autoantibodies and Markers of Systemic Inflammation in Rheumatoid Arthritis Patients

“…Keskin et al [21] found that the measurement of serum anti-MCV levels is useful for diagnosis of RA and combined use of anti-MCV and RF antibodies may be more useful prognostic factor than RF and anti-CCP. The discrepancy between various studies can be explained by the difference in: (i) patient populations [22], (ii) early RA [23,24] or established RA [12,25], or (iii) number of cases studied [15].…”