Antibodies to the neural cell adhesion molecule disrupt functional recovery in injured nerves

Remsen, Laura G.; Strain, George M.; Newman, Melissa; Satterlee, Nancy; Daniloff, Joanne Kelsch

doi:10.1016/0014-4886(90)90038-t

Cited by 10 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on studies to date of adult nervous system regeneration, axon processes that are regenerating have been shown to express cell adhesion molecules that are commonly seen only during development. For example, in the peripheral nervous system L1 has been shown to be involved during regeneration (Nieke and Schachner, 1985; Martini and Schachner, 1988; Remsen et al, 1990). In addition, Aubert et al (1998) showed that L1 expression was upregulated on regenerating cholinergic axons during axonal elongation.…”

Section: Discussionmentioning

confidence: 99%

Expression of L1 decreases during postnatal development of rat spinal cord

Akopians

Runyan

Phelps

2003

J of Comparative Neurology

View full text Add to dashboard Cite

L1 is a cell adhesion molecule that is highly expressed on developing axons and is associated with neurite outgrowth, guidance, and fasciculation. In this study we systematically examined L1 expression at all spinal levels across eight postnatal ages to detect regional and developmental differences. We observed striking changes in the developmental pattern of L1 expression between birth (P0) and adult ages, with intense L1-immunopositive axons prevalent throughout the funiculi at P0 compared with predominantly L1-immunonegative funicular axons in adults. At all ages and spinal levels examined, some L1-positive dorsal root afferents entered the spinal cord, coursed in Lissauer's tract, and projected into the superficial dorsal horn and the dorsal columns, as well as across the dorsal commissure. Additional L1-positive axons were detected consistently around the perimeter of the spinal cord, in the dorsolateral funiculus, and adjacent to the central canal. While specific L1-labeled axons were detected at all ages, a pattern of segmental variation was observed within animals, with the highest levels of L1 expression detected in lumbar and sacral segments and the lowest in cervical spinal cord. The pattern of L1 immunoreactivity was compared to that of the growth-associated protein GAP-43 and the results indicated colabeling of most axons. These observations demonstrate that L1 is expressed on immature axons well into postnatal development, possibly until they have completed their differentiation. Furthermore, the L1-positive axons that continue to be detected in adults are likely to be either unmyelinated or sprouting axons.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of L1 decreases during postnatal development of rat spinal cord

Akopians

Runyan

Phelps

2003

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…Both NCAM and L l have been shown to be involved in peripheral nervous system (PNS) regeneration (Nieke and Schachner, 1985;Martini and Schachner, 1988;Seilheimer and Schachner, 1988;Remsen et al, 1990). In the few CNS systems that do successfully regenerate, data also demonstrate a likely role for adhesive molecules.…”

Section: Immunohistochemical Staining For Brightfield Observationsmentioning

confidence: 90%

Sprouting adult CNS cholinergic axons express nile and associate with astrocytic surfaces expressing neural cell adhesion molecule

1996

View full text Add to dashboard Cite

To assess the cellular and molecular substrates for cholinergic axon growth in the adult central nervous system (CNS), we implanted grafts of control and nerve growth factor (NGF)-producing genetically modified fibroblasts within the striatum of rats. Sprouting cholinergic axonal processes that grew into grafts of NGF-producing fibroblasts were fasciculated and followed the surface of astrocytic processes for long distances within the grafts. The close and long distance anatomical relationship between the sprouted axons and the astrocytes supported previous ultrastructural evidence that astrocytes may serve as a cellular substrate for sprouting cholinergic axons in vivo. The sprouted axon processes were associated with the expression of nerve growth factor-inducible large external (NILE) glycoprotein on their surfaces. NILE expression was not seen in control grafts where there was an absence of cholinergic ingrowth. NILE has been demonstrated to play a role in axon fasciculation in a number of other neural systems. The astrocytic processes in both control and NGF-producing fibroblast grafts expressed neural cell adhesion molecule (NCAM), suggesting that NCAM-mediated adhesion may be responsible for the close relationship between the axons and astrocytes within the grafts. NGF-induced heterotypic interactions between neuronal NILE and astroglial NCAM may also be required for adult cholinergic axonal sprouting.

show abstract

Immunohistochemical localization of cell adhesion molecules and cell-cell contact proteins during regeneration of the rat optic nerve induced by sciatic nerve autotransplantation

Dezawa

Nagano

1996

Anat. Rec.

View full text Add to dashboard Cite

Antibodies to the neural cell adhesion molecule disrupt functional recovery in injured nerves

Cited by 10 publications

References 13 publications

Expression of L1 decreases during postnatal development of rat spinal cord

Expression of L1 decreases during postnatal development of rat spinal cord

Sprouting adult CNS cholinergic axons express nile and associate with astrocytic surfaces expressing neural cell adhesion molecule

Immunohistochemical localization of cell adhesion molecules and cell-cell contact proteins during regeneration of the rat optic nerve induced by sciatic nerve autotransplantation

Contact Info

Product

Resources

About