Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of
            <i>Streptococcus pneumoniae</i>

Jomaa, Maha; Yuste, José; Paton, James C.; Jones, Christopher; Dougan, Gordon; Brown, Jeremy S.

doi:10.1128/iai.73.10.6852-6859.2005

Cited by 76 publications

(60 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The need for iron acquisition makes transport-negative mutants less virulent (9). In addition, the surface-exposed iron transport lipoproteins are strong antigens, and their use for active and passive immunization protects mice against invasive S. pneumoniae disease (25).…”

Section: Discussionmentioning

confidence: 99%

Albomycin Uptake via a Ferric Hydroxamate Transport System ofStreptococcus pneumoniaeR6

Pramanik

Braun

2006

J Bacteriol

View full text Add to dashboard Cite

The antibiotic albomycin is highly effective against Streptococcus pneumoniae, with an MIC of 10 ng/ml. The reason for the high efficacy was studied by measuring the uptake of albomycin into S. pneumoniae. Albomycin was transported via the system that transports the ferric hydroxamates ferrichrome and ferrioxamine B. These two ferric hydroxamates antagonized the growth inhibition by albomycin and salmycin. Cross-inhibition of the structurally different ferric hydroxamates to both antibiotics can be explained by the similar iron coordination centers of the four compounds.

show abstract

Section: Discussionmentioning

confidence: 99%

Albomycin Uptake via a Ferric Hydroxamate Transport System ofStreptococcus pneumoniaeR6

Pramanik

Braun

2006

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Immunization with PiaA and PiuA elicits protective antibodies that recognize these surface-exposed proteins, and recently these antibodies have been demonstrated to promote bacterial opsonophagocytosis rather than inhibit iron transport (Jomaa et al, 2005).…”

Section: Lipoproteins: Implications In Substrate Transport and Virulencementioning

confidence: 99%

Versatility of pneumococcal surface proteins

2006

View full text Add to dashboard Cite

Surface-exposed proteins are key players during the infectious process of pathogenic bacteria. The cell surface of the Gram-positive human pathogen Streptococcus pneumoniae is decorated not only by typical Gram-positive surface proteins, but also by a family of proteins that recognizes the phosphorylcholine of the lipoteichoic and teichoic acids, namely the choline-binding proteins, and by non-classical surface proteins that lack a leader peptide and membrane-anchor motif. A comprehensive understanding of how microbial proteins subvert host immunity or host protein functions is a prerequisite for the development of novel therapeutic strategies to combat pneumococcal infections. This article reviews recent progress in the investigation of the versatility and sophistication of the virulence functions of surface-exposed pneumococcal proteins.

show abstract

“…The Piu and Pia transport systems are essential for iron uptake and full virulence in murine systemic and pulmonary models of infection (9). Immunization of mice with purified recombinant PiuA and PiaA showed that they are capable of eliciting protective im-munity against systemic challenge with S. pneumoniae (10), mediated by their likely presence on the bacterial cell surface (46) and hence ability to act as opsonins (26). Human sera from patients with pneumococcal septicemia caused by 8 different capsular serotypes contained anti-PiuA and anti-PiaA antibodies, which cross-reacted with recombinant PiuA and PiaA from a single capsular serotype (53), indicating that this immune response was serotype independent.…”

mentioning

confidence: 99%

Distribution and Genetic Diversity of the ABC Transporter Lipoproteins PiuA and PiaA withinStreptococcus pneumoniaeand Related Streptococci

Whalan

Funnell²,

Bowler

et al. 2006

J Bacteriol

View full text Add to dashboard Cite

Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide. The existence of approximately 90 antigenically distinct capsular serotypes has greatly complicated the development of an effective pneumococcal vaccine. Virulence-associated proteins common and conserved among all capsular types now represent the best strategy to combat pneumococcal infections. PiuA and PiaA are the lipoprotein components of two pneumococcal iron ABC transporters and are required for full virulence in mouse models of infection. Here we describe a study of the distribution and genetic diversity of PiuA and PiaA within typical and atypical S. pneumoniae, Streptococcus oralis, and Streptococcus mitis strains. The genes encoding both PiuA and PiaA were present in all typical pneumococci tested, (covering 20 and 27 serotypes, respectively). The piuA gene was highly conserved within the typical pneumococci (0.3% nucleotide divergence), but was also present in "atypical" pneumococci and the closely related species S. mitis and S. oralis, showing up to 10.4% nucleotide divergence and 7.5% amino acid divergence from the typical pneumococcal alleles. Conversely, the piaA gene was found to be specific to typical pneumococci, 100% conserved, and absent from the oral streptococci, including isolates of S. mitis known to possess pneumolysin and autolysin. These are desirable qualities for a vaccine candidate and as a diagnostic tool for S. pneumoniae.

show abstract

Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of Streptococcus pneumoniae

Cited by 76 publications

References 29 publications

Albomycin Uptake via a Ferric Hydroxamate Transport System ofStreptococcus pneumoniaeR6

Albomycin Uptake via a Ferric Hydroxamate Transport System ofStreptococcus pneumoniaeR6

Versatility of pneumococcal surface proteins

Distribution and Genetic Diversity of the ABC Transporter Lipoproteins PiuA and PiaA withinStreptococcus pneumoniaeand Related Streptococci

Contact Info

Product

Resources

About