1989
DOI: 10.1128/mcb.9.9.3904
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Antibodies to synthetic peptide from the residue 33 to 42 domain of c-Ha-ras p21 block reconstitution of the protein with different effectors.

Abstract: Residues 32 to 40, which are conserved among ras proteins from different species, are likely to participate in interactions with the p21 effector system. With the goal of understanding the structural basis of the regulatory functions of c-Ha-ras p21, we produced rabbit antisera against a synthetic peptide corresponding to amino acids 33 to 42 of the protein. The affinity-purified antibodies interacted specifically with p21 and with the antigenic peptide. The epitope recognized by the antibodies appeared to be … Show more

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Cited by 19 publications
(10 citation statements)
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References 26 publications
(35 reference statements)
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“…Our results are somewhat different, in that Ras-GAP seems to promote cytoskeletal changes in a Ras-independent fashion. Indeed, stress fiber assembly induced by Ras-GAP was not blocked by injection of the neutralizing antibody Y13-259, which has been shown to prevent Ras-GAP from binding to Ras (42). This is also consistent with the very low level of active GTP-bound Ras which must be left in Swiss 3T3 cells after serum deprivation.…”
Section: Discussionsupporting
confidence: 60%
See 1 more Smart Citation
“…Our results are somewhat different, in that Ras-GAP seems to promote cytoskeletal changes in a Ras-independent fashion. Indeed, stress fiber assembly induced by Ras-GAP was not blocked by injection of the neutralizing antibody Y13-259, which has been shown to prevent Ras-GAP from binding to Ras (42). This is also consistent with the very low level of active GTP-bound Ras which must be left in Swiss 3T3 cells after serum deprivation.…”
Section: Discussionsupporting
confidence: 60%
“…GAPSH2-SH3-SH2 (amino acids 182 to 442) was obtained and cloned into pGEX-2T as described previously (11). GST fusion proteins were purified by affinity chromatography (11), and Ha-RasK12 was purified by anion-exchange chromatography (42).…”
Section: Methodsmentioning
confidence: 99%
“…RhoAV14 and Cdc42V12 were puri®ed as described (Ridley and Hall). HaRasK12 was puri®ed by anion exchange chromatography (Rey et al, 1989). RhoAV14 (3 mg/ml), Cdc42V12 (3 mg/ml) and Ha-RasK12 (2 mg/ml) proteins were injected into the cytoplasm immediately after injection of IgG, mAb200 or Y13-259 (10 mg/ml).…”
Section: Mab200 Speci®cally Induces Apoptosis Of Tumour But Not Of Nmentioning
confidence: 99%
“…GAP stimulates the conversion of Ras-GTP to Ras-GDP, thereby downregulating the biological function of Ras proteins. GAP interacts with Ras at the effector binding domain (1,10,28) and may act as an effector as well as a negative regulator (23,31,42). Another set of recent studies suggests that the catalytic fragment of the neurofibromatosis gene might function similarly to GAP (2,22).…”
mentioning
confidence: 99%