Antibodies to phospholipids and liposomes: binding of antibodies to cells

Fogler, William E.; Swartz, Glenn M.; Alving, Carl R.

doi:10.1016/0005-2736(87)90215-x

Cited by 23 publications

(14 citation statements)

References 20 publications

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“…The latter antibodies reportedly have a strong choline-binding (trimethylammonium-binding) subsite Monoclonal antibodies to liposomal phospholipids [1401. but the possibility that the latter antibodies also did not bind to mouse peritoneal macrophagcs that have a phosphate-binding subsitc has not been examwere kept in suspension, but large amounts of antibodincd. The data appear to be consistent overall with the ics did bind to adhercnt macrophagcs [183] (Fig. 5).…”

Section: °4supporting

confidence: 73%

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Immunologic aspects of liposomes: presentation and processing of liposomal protein and phospholipid antigens

Alving

1992

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

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107

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Section: °4supporting

confidence: 73%

“…Another subsite that occurrence of macrophagc adherence or after damage recognizes serine was also proposed based on hapten to the cell surface protein integrity. antibody binding to inhibition by serine of polyclonal antiserum against cell surface phospholipids can occur [183]. phosphatidylscrine 1160].…”

mentioning

confidence: 99%

Immunologic aspects of liposomes: presentation and processing of liposomal protein and phospholipid antigens

Alving

1992

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

Self Cite

107

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“…It has been reported that high concentrations of CCR5 in HeLa lines are associated with lower neutralization efficacy with X5 antibody (12), and anti-PIP antibodies may be similar to X5 in this respect. It is well known that high protein/lipid ratios can sterically inhibit binding of antibodies to lipids on cells or lipoproteins (4,5,13,16). In a striking example of the absence of binding to whole cells, antibodies to PIP did not bind to nonadherent cultured macrophages, as determined by indirect immunofluorescence (16).…”

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confidence: 95%

“…Four complement-fixing murine MAbs to PIP, selected for their abilities to react with liposomes containing PIP but not with liposomes lacking PIP, have been extensively studied (2,3,6,16,17,30,32,33). The anti-PIP antibodies are characterized by the ability to react with various types of phosphorylated molecules, including certain closely related anionic phospholipids that have charged nonzwitterionic phosphate groups, such as CL (2), and also with denatured DNA (30).…”

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confidence: 99%

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Monoclonal Antibodies to Phosphatidylinositol Phosphate Neutralize Human Immunodeficiency Virus Type 1: Role of Phosphate-Binding Subsites

Brown

Karasavvas

Beck

et al. 2007

J Virol

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Both a murine monoclonal antibody to phosphatidylinositol phosphate (PIP) and a human monoclonal antibody (4E10) that is known to have broadly neutralizing capabilities against primary isolates of human immunodeficiency virus type 1 (HIV-1) bound to PIP, as determined by enzyme-linked immunosorbent assay. Each of the antibodies had antigen subsite binding specificities in aqueous medium for small phosphatecontaining molecules and for inositol. The anti-PIP monoclonal antibody inhibited infection by two HIV-1 primary isolates in neutralization assays employing primary human peripheral blood mononuclear cells. The data suggest that PIP or related lipids having free phosphates could serve as targets for the neutralization of HIV-1.

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Cytochemical studies of lipid metabolism: Immunogold probes for lipoprotein lipase and cholesterol

Blanchette‐Mackie

1989

Am. J. Anat.

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In this article, cytochemical methods are presented for the study of lipid metabolism both in normal cells and in mutant cells with genetic disorders characterized by abnormal lipid metabolism. The benefit of using an immunocytochemical approach to the study of lipase in tissues is discussed, and a review is presented of the results on immunolocalization of lipoprotein lipase in cardiac tissue of normal mice. Immunocytochemical techniques are applied to the study of lysosomal proliferation in hepatocytes from liver of mutant mice with a genetic defect responsible for the lack of hepatic lipase and lipoprotein lipase activity in these animals. Localization of lipids in tissues with structural techniques has been an area of great interest to our laboratory for many years. Attention is called to the development of a technique for the visualization of fatty acids as a function of their ionization state and the production of fatty-acid myelin figures in membranes. Results on the use of filipin to detect unesterified cholesterol in membranes are reviewed. Filipin produces fluorescent filipin-cholesterol complexes but also perturbs cell membranes. Application of this cytochemical probe, in combination with immunocytochemistry of lysosomes, produced useful information on defects in low-density lipoprotein-derived cholesterol translocation in mutant human fibroblasts. Initial results on the application of immunological techniques to the study of cholesterol in lipid model systems indicate a novel approach, which may be applicable to specialized cell systems. Recent advances in cryoultramicrotomy and development of immunoprobes present valuable opportunities for the structural assessment of lipids and lipases in cell organelles and cell membranes.

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Antibodies to phospholipids and liposomes: binding of antibodies to cells

Cited by 23 publications

References 20 publications

Immunologic aspects of liposomes: presentation and processing of liposomal protein and phospholipid antigens

Immunologic aspects of liposomes: presentation and processing of liposomal protein and phospholipid antigens

Monoclonal Antibodies to Phosphatidylinositol Phosphate Neutralize Human Immunodeficiency Virus Type 1: Role of Phosphate-Binding Subsites

Cytochemical studies of lipid metabolism: Immunogold probes for lipoprotein lipase and cholesterol

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