Antibodies toEubacteriumandPeptostreptococcusspecies and the estimated probability of Crohn's disease

Abstract

Anaerobic coccoid rods belonging to species of Eubacterium and Peptostreptococcus agglutinate more frequently with sera from patients with Crohn's disease than with sera from patients suffering from other diseases and from healthy subjects. Results of agglutination test with four strains of coccoid anaerobes were used to estimate the probability that a patient suffers from Crohn's disease. The data on healthy subjects and patients with Crohn's disease were subjected to logistic discriminant analysis. With the methods and interpretation described, 52% of the patients with Crohn's disease were recognized as 'definite' or 'probable' Crohn's disease and 14% as 'suspected'. Only 1% of the healthy subjects were classified as 'suspected' and non as 'definite' or 'probable' Crohn's disease.

Serum agglutinins toEubacteriumandPeptostreptococcusspecies in Crohn's and other diseases

“…The strength of agglutination should be considered when the tests with four strains were used to obtain one combined result. This was achieved by using discriminant analysis to estimate the probability that a patient suffers from Crohn's disease ( Van de Merwe, Schmitz & Wensinck, 1981). In the present report, sera with agglutination reactions giving an a posteriori probability > 0 95 were considered positive.…”

“…The agglutination of these strains by sera from healthy subjects and from patients with Crohn's and other diseases is reported here. The results have been used to estimate the probability that a patient suffers from Crohn's disease by means of an interpretation based on discriminant analysis (Van de Merwe, Schmitz & Wensinck, 1981). Subsequently, sera submitted for the establishment of the diagnosis of Crohn's disease have also been interpreted in this way.…”

Serum Antibodies to Peptidoglycan-Polysaccharide Complexes from the Anaerobic Intestinal Flora in Patients with Crohn’s Disease

“…Forty sera were assigned to one of four groups: group 1, CD with a positive agglutination reaction; group 2, CD with a negative agglutination reaction; group 3, not CD with a positive agglutination reaction; group 4, not CD with a negative agglutination reaction. The agglutina tion reaction was called positive when the posterior probability of CD (p value) calculated from the four agglutination reactions was 3= 0.80 and negative when the p value was < 0 .8 0 [3]. Reactions with a p s* 0.80 support the diagnosis CD.…”

“…The con centration of the PPC fraction in the feces of patients with CD was lower compared with that of HS [1], Other studies from our labo ratory showed that sera from patients with CD agglutinated strains of Coprococcus comes, Peptostreptococcus productus and Eubacterium contortum, present in the intesti nal anaerobic flora, more often and strongly than sera from patients with ulcerative coli tis, other diseases, and HS. The agglutina tion reactions are used as a diagnostic test for CD [2][3][4],In this study we investigated the bacterial origin of the soluble intestinal PPC and the occurrence of specific antibodies to PPC in sera from patients with CD and control HS. …”

Agglutinins to anaerobic bacteria in crohn's disease and in indian patients with diarrhoea

“…In Rotterdam, a much higher incidence of agglutinins to anaerobic bacteria of Eubacterium and Peptostreptococcus species was found in the sera of patients with Crohn's disease than in those from healthy controls (Wensinck, 1975 and1976;Van de Merwe, Schmitz and Wensinck, 1981;Wensinck and Van de Merwe, 1981). These workers also identified increased numbers of these bacteria in the stools of patients with the disease .…”