Antibodies to Herpes-Simplex Virus in the Cerebrospinal Fluid of Patients With Herpetic Encephalitis

MacCallum, F. O.; Chinn, I. J.; Gostling, J.V.T.

doi:10.1099/00222615-7-3-325

Cited by 60 publications

(14 citation statements)

References 3 publications

(4 reference statements)

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“…Herpes simplex virus (HSV) is one of the causative agents of viral encephalitis in children. Herpes simplex encephalitis (HSE) is usually diagnosed in cerebrospinal fluid (CSF) samples by virus culture and antigen detection [1,2], or by antibody level correlation with serum [3][4][5][6][7]. On many occasions, all these tests may be negative although clinical diagnosis may indicate HSV encephalitis [8].…”

Section: Accepted For Publication 13 August 1992mentioning

confidence: 99%

Detection of Herpes Simplex Virus DNA in Cerebrospinal Fluid of Saudi Pediatric Patients with Encephalitis by the Polymerase Chain Reaction

Al‐Ahdal¹,

Kessie²,

Ahmed³

et al. 1993

Ann Saudi Med

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Section: Accepted For Publication 13 August 1992mentioning

confidence: 99%

Detection of Herpes Simplex Virus DNA in Cerebrospinal Fluid of Saudi Pediatric Patients with Encephalitis by the Polymerase Chain Reaction

Al‐Ahdal¹,

Kessie²,

Ahmed³

et al. 1993

Ann Saudi Med

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“…Three methods are currently accepted for early diagnosis: immunofluorescent examination (and/or virus culture) of brain tissue obtained by biopsy [MacCallum et al, 1964;Buckley and MacCallum, 19671, demonstration of a specific intrathecal immune response t o herpes simplex virus (HSV) in lumbar cerebrospinal fluid (CSF) [MacCallum et al, 1974;Klapper et al, 19811, or the detection of HSV specific glycoprotein antigen in CSF [Lakeman et al, 19871. In terms of early diagnosis, however, each of these procedures has disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

Klapper

Cleator

Dennett

et al. 1990

Journal of Medical Virology

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Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumbar cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR-SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant "non-invasive" method for the diagnosis of HSE.

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“…Serological diagnostics has widely been adopted to determine antibody response in HSE (MacCallum et al, 1974;Koskiniemi and Vaheri, 1982). Several studies have reported that, PCR as well as detection of specific antibodies in sera and CSF samples should be retrospectively analyzed for the diagnosis of HSE, so as to understand pathogenesis and follow the course of the disease (Akçali et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Identification of an immunodominant epitope in glycoproteins B and G of herpes simplex viruses (HSVs) using synthetic peptides as antigens in assay of antibodies to HSV in herpes simplex encephalitis patients

Ss¹,

Nh²,

Nn³

et al. 2014

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Summary. -Herpes simplex encephalitis (HSE) is a severe viral infection of the central nervous system (CNS). Assay of antibody response is widely used in diagnostics of HSE. The aim of this study was to identify an immunodominant epitope determining the antibody response to herpes simplex viruses (HSVs) in cerebrospinal fluid (CSF) of HSE patients. The synthetic peptides that resembled type-common as well as type-specific domains of glycoproteins B (gB) and G (gG) of these viruses were evaluated for binding with IgM and IgG antibodies in CSF samples from HSE and non-HSE patients in ELISA. The QLHDLRF peptide, derived from gB of HSV was found to be an immunodominant epitope in the IgM and IgG antibody response. The patients with confirmed and suspected HSE showed in ELISA against this peptide 26% and 23% positivities for IgM, 43% and 37% positivities for IgG and 17% and 15% for both IgM and IgG antibodies, respectively. The total positivities of 86% and 75% for both IgM and IgG antibodies were obtained in the patients with confirmed and suspected HSE, respectively. These results demonstrate that a synthetic peptide-based diagnostics of HSE can be an efficient and easily accessible alternative. This is the first report describing the use of synthetic peptides derived from HSVs in diagnostics of HSE using patientsʹ CSF samples.

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Antibodies to Herpes-Simplex Virus in the Cerebrospinal Fluid of Patients With Herpetic Encephalitis

Cited by 60 publications

References 3 publications

Detection of Herpes Simplex Virus DNA in Cerebrospinal Fluid of Saudi Pediatric Patients with Encephalitis by the Polymerase Chain Reaction

Detection of Herpes Simplex Virus DNA in Cerebrospinal Fluid of Saudi Pediatric Patients with Encephalitis by the Polymerase Chain Reaction

Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

Identification of an immunodominant epitope in glycoproteins B and G of herpes simplex viruses (HSVs) using synthetic peptides as antigens in assay of antibodies to HSV in herpes simplex encephalitis patients

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