Antibodies to Haemophilus equigenitalis in bovine sera

Corbel, M.J.; Brewer, R.A.

doi:10.1136/vr.106.2.35-a

Cited by 4 publications

(4 citation statements)

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“…The serum was collected 7 days after the final injection and stored at -20 'C until required. Bovine sera from apparently healthy animals and cases of reproductive failure were collected as described previously (Corbel & Brewer, 1980).…”

Section: Antiseramentioning

confidence: 99%

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Characterization of the major antigens of Haemophilus equigenitalis (contagious equine metritis organism)

Corbel

Brewer

1982

J. Hyg.

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SUMMARYImmunoelectrophoresis of ultrasonically disrupted Haenophilus equigenitalis (contagious equine metritis organism) cells against rabbit and equine antisera disclosed at least 11 precipitating antigens. Two of these, a polysaccharide and a lipopolysaccharide-protein complex, were of high molecular weight and located on the cell surface. The remaining antigens were intracellular and were small-to medium-sized proteins.The surface antigens were the most significant in relation to the serological response in infected horses. They also reacted with sera from apparently healthy cattle, but the reason for this was not determined. No serological cross-reaction between H. equigenitalis and species of Achromobacter and Moraxella was detected.

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Section: Antiseramentioning

confidence: 99%

“…Recently, during the course of studies on the possible role of H. equigenitalis as an agent of reproductive failure in cattle, antibodies to this organism were detected in an unexpectedly high proportion of bovine serum samples (Corbel & Brewer, 1980).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the major antigens of Haemophilus equigenitalis (contagious equine metritis organism)

Corbel

Brewer

1982

J. Hyg.

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“…Approximately 600 cattle tested in the United Kingdom had titers between 1:10 to 1:160 to the CEMO with most of the titers ranging from 1:20 to 1:40 (17). The titers did not correlate with reproductive failure or clinical signs similar to those seen in CEMO infected horses (17).…”

Section: There Have Been 18 Isolations Of Cemo In Young Nonbreeding Amentioning

confidence: 94%

“…The significance of finding serum agglutinins to CEMO in cattle and humans is unclear. The authors speculated that these titers may be from undetermined organisms with one or more antigens in common with CEMO (17,126). Isolation of CEMO was not successful in either the bovine or human cases (17,126).…”

Section: There Have Been 18 Isolations Of Cemo In Young Nonbreeding Amentioning

confidence: 99%

Interaction of equine neutrophils and the contagious equine metritis organism

Bertram

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INTRODUCTION 20 MATERIALS AND METHODS 21 Animals 21 Blood collection 21 Leukocyte separation 21 Electron microscopic examination 24 Morphometry 25 RESULTS 28 Granulocyte separation 28 Ultrastructural examination 28 iii Morphometric examination 40 DISCUSSION 42 PHAGOCYTOSIS AND INTRACELLULAR KILLING OF THE CONTAGIOUS EQUINE METRITIS ORGANISM BY EQUINE NEUTROPHILS IN SERUM 45 ABSTRACT 46 INTRODUCTION 47 MATERIALS AND METHODS 49 Serum 49 Neutrophils 49 Bacteria 50 Phagocytosis assay 51 Intracellular killing assay 51 Cell associated bacteria 52 Electron microscopy 52 Statistical analysis 53

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