Antibodies to collagen in scleroderma

Mackel, A M; DeLustro, Frank; Harper, Frank E.; Roy, Eleanor

doi:10.1002/art.1780250505

Cited by 100 publications

(37 citation statements)

References 28 publications

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“…Gabrielli et al [3] detected anti-CIV antibodies in 68% SSc patients; Mackel et al [4] detected anti-CIV antibodies in 50% of their SSc patients and anti-CI in 60%. A different patient population (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…While the presence of antibodies to denatured collagens seems to have been well established by different authors, conflicting results are obtained when antibodies to native collagens are measured [3,4]. The aim of this study was to evaluate the frequency and specificity of antibodies to native and denatured CI, CIV and CV in patients affected by SSc and PRP.…”

Section: Introductionmentioning

confidence: 96%

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Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon

Riente

Marchini

Dolcher

et al. 1995

Clinical and Experimental Immunology

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SUMMARYThe frequency and specificity of antibodies to native and denatured coUagens were evaluated in systemic sclerosis (SSc) and in primary Raynaud's phenomenon (PRP) by direct and competitive ELISA. Antibodies reactive with denatured collagen type I (CI) were found in 43% of the SSc sera, and anti-CIV and anti-CV in 31%. In PRP, anti-CI, anti-CIV and anti-CV antibodies were detected in 8% of patient sera. Anti-CI, anti-CIV and anti-CV antibodies reacted with determinants expressed on the native as well as on the denatured molecule. Anti-CI and anti-CIV were cross-reactive; a reactivity with CII and a lower one with CV were detected. Anti-CV antibodies also reacted with CI and CII and, in a smaller proportion of cases, with CIV. Anti-collagen antibodies, affinity-purified from blotted collagen IV and V and cyanogen bromide (CBr)-digested CI, displayed the cross-reactivities shown by inhibition studies on sera. Moreover, antibodies eluted from a CBr fragment of CI reacted with the other CBr fragments as well. These data show that one-third of SSc sera contain antibodies that react with epitopes expressed on native as well as on heat-denatured CI, CII, CIV and CV, and therefore have the potential to bind collagens in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon

Riente

Marchini

Dolcher

et al. 1995

Clinical and Experimental Immunology

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“…Modelos de sensibilização com os colágenos tipo II, IX e XI mostraram que estas proteínas possuem funções imunogênicas e artritogênicas 5,10,12 . A importância dos anticorpos anticolágeno nas DDTC já foi observada, principalmente a freqüência de anticolágenos I, II, IV e V em pacientes com vasculites e lúpus eritematoso sistêmico 19 ; anticolágenos I e IV na esclerose sistêmica 16 ; e anticorpos anticolágeno VII na Síndrome de Sjörgren 6 . Dentre os diversos colágenos, o do tipo V parece estar envolvido na patogênese das DDTC.…”

Section: Discussionunclassified

Alterações esofágicas em modelo de esclerodermia induzido por colágeno V em coelhos

Julião

Pereira

Anjos

et al. 2007

Rev. Med. (São Paulo)

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Julião GPS, Pereira DD, Anjos DM dos, Teodoro WR, Yoshinari NH, Velosa APP. Alterações esofágicas em modelo de esclerodermia induzido por colágeno V em coelhos. Rev Med (São Paulo). 2007 jul.-set.;86(3):163-73. RESUMO:Introdução: a esclerose sistêmica progressiva acomete diversas vísceras, das quais o esôfago é uma das mais freqüentemente lesadas. No entanto, o mecanismo de autoimunidade nesta enfermidade é ainda desconhecido. Objetivo: avaliar morfologicamente esôfagos de animais após imunização com colágeno tipo V, correlacionando-os com as alterações observadas em humanos. Materiais e métodos: foram analisados esôfagos de coelhos do modelo de esclerodermia induzido por colágeno V. As amostras foram analisadas morfologicamente pelas técnicas de H&E, Tricrômico de Masson, Picro Sirius e Imunofl uorescência. Resultados: observou-se aumento progressivo das fi bras colágenas, caracterizado por alterações no epitélio, submucosa, camada muscular e adventícia. A análise da imunofl uorescência revelou a existência de remodelamento dos diferentes tipos de colágeno da matriz. Discussão: o estudo de espécimes esofágicos em humanos é difícil. Na maioria das vezes só é possível analisá-lo em fases avançadas da doença. No modelo estudado acreditamos que as alterações observadas estão relacionadas a um processo inicial da doença e que o estudo de animais sacrifi cados após um tempo maior irá revelar uma fase mais avançada da doença. DESCRITORES: Esclerodermia localizada. Esofagopatias. Modelos animais. Coelhos. Colá-geno tipo V. Avanço da doença.

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“…urc involved in the fibrosis remains lo be proved. Also, high levels of antibodies to native and denatured type I, type 111 and lypc IV collagens are present in TOS piuients \l% as well as in scleroderma patients [20]. Anticollagcn antibodies are characleristic of many inflammatory diseases of the connective tissues, but their role in the etiopathogcnesis of the fibrotic process remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Expression of types I, III and IV collagen genes in fibrotic skin and nerve lesions of toxic oil syndrome patients

Gómez-Reino

Sandberg

Carreira

et al. 1993

Clinical and Experimental Immunology

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SUMMARYWe bave studied the skin and nerve fibrosis in toxic oil syndrome by //( situ hybridization tising specific cDN A probes for types I, III, and IV collagcn.s, Eibroblasts with bigh levels of type I and III collagen mRNA were observed in biopsies from librotic skin areas. Similarly, type IV collagen mRNA was abundant in cells within the librotic process ofthe nerves. These results suggest that the excessive accumulation of collagen in toxic oil syndrome results from transcriptional activation of collagen genes in a subpopulation of tibrobiasts.

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Antibodies to collagen in scleroderma

Cited by 100 publications

References 28 publications

Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon

Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon

Alterações esofágicas em modelo de esclerodermia induzido por colágeno V em coelhos

Expression of types I, III and IV collagen genes in fibrotic skin and nerve lesions of toxic oil syndrome patients

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