Antibodies to Borrelia turicatae in Experimentally Infected Dogs Cross-React with Borrelia burgdorferi Serologic Assays

Gettings, Jenna R.; López, Job E.; Krishnavahjala, Aparna; Armstrong, Brittany A.; Thompson, Alec T.; Yabsley, Michael J.

doi:10.1128/jcm.00628-19

Cited by 19 publications

(20 citation statements)

References 27 publications

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“…Moreover, dog 2 was the only animal without anemia, and showed signs pointing toward chronic infection, such as leucocytosis and thrombocytopenia. A recent experimental infection of six dogs with the RF species B. turicatae lends support to the cross-reactivity hypothesis, as five of the dogs showed positive results in a whole-cell-based test (IFA) for LB borreliae, three of them had slight positive reactions in a Quant-C6-ELISA (13 to 24 U/mL), but all six dogs reacted negatively in the SNAP ® 4Dx ® test (as shown for both dogs and the cat in the present study) [ 21 ]. A limitation of this study was that only one time point post infection (43 dpi) was tested.…”

Section: Resultssupporting

confidence: 72%

“…Therefore, the SNAP ® 4Dx ® test utilizing a C6 peptide of B. burgdorferi can currently be regarded as the only serological test for LB in dogs not cross-reacting with RF borreliae. At present, there are no commercially available serological tests for RF borreliosis in dogs that could improve the diagnosis, i.e., based on GlpQ and BipA [ 21 ]. Another RF Borrelia species in Europe, B. miyamotoi , may represent also a diagnostic challenge in terms of cross-reactivity with LB borreliae.…”

Section: Resultsmentioning

confidence: 99%

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First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe

Margos¹,

Pantchev

Globokar

et al. 2020

Microorganisms

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Canine cases of relapsing fever (RF) borreliosis have been described in Israel and the USA, where two RF species, Borrelia turicatae and Borrelia hermsii, can cause similar clinical signs to the Borrelia persica in dogs and cats reported from Israel, including fever, lethargy, anorexia, thrombocytopenia, and spirochetemia. In this report, we describe the first clinical cases of two dogs and a cat from Spain (Cordoba, Valencia, and Seville) caused by the RF species Borrelia hispanica. Spirochetes were present in the blood smears of all three animals, and clinical signs included lethargy, pale mucosa, anorexia, cachexia, or mild abdominal respiration. Laboratory findings, like thrombocytopenia in both dogs, may have been caused by co-infecting pathogens (i.e., Babesia vogeli, confirmed in one dog). Anemia was noticed in one of the dogs and in the cat. Borrelia hispanica was confirmed as an infecting agent by molecular analysis of the 16S rRNA locus. Molecular analysis of housekeeping genes and phylogenetic analyses, as well as successful in vitro culture of the feline isolate confirmed the causative agent as B. hispanica.

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Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe

Margos¹,

Pantchev

Globokar

et al. 2020

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Interestingly, this incidence coincided with the seroprevalence rate of B. burgdorferi among dog populations examined in Texas by this and previous studies [27,29]. It also should be mentioned that exposure to tick-borne relapsing fever Borrelia occurs in Texas; however, based upon a recent publication the B. burgdorferi C6 peptide-based SNAP test did not result in a false positive in experimentally infected dogs [51]. Also, in this study a travel history could not be established for the B. burgdorferi-positive dog.…”

Section: Discussionsupporting

confidence: 83%

Serological prevalence of six vector-borne pathogens in dogs presented for elective ovariohysterectomy or castration in the South central region of Texas

Fudge¹,

Boyanowski²,

Page³

et al. 2020

BMC Vet Res

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Background Most vector-borne pathogens cause zoonotic diseases. These zoonoses often have wild animal reservoirs that play a significant role in disease epidemiology. However, pet animals have also been implicated in transmission of zoonotic agents to humans. To exemplify, dogs are competent reservoir hosts for several zoonotic vector-borne bacteria and protozoa. Despite that vector-borne diseases can be life-threatening for both pets and humans, studies on pathogen seroprevalence are very limited. Therefore, the objective of this study was to determine the serological prevalence of six zoonotic vector-borne agents in dogs from the South Central region of Texas (US). Electronic medical records of dogs, presenting over 2014–2019 for elective ovariohysterectomy or castration at a high volume spay and neuter clinic, were reviewed for serological testing. Sera from 418 dogs were tested for the Dirofilaria immitis antigen, and antibodies to Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis, and Ehrlichia ewingi, using a commonly available commercial test kit. Descriptive statistics were computed to characterize the respective seroprevalence rates of the dog population. The study involved 192 (46%) male and 226 (54%) female dogs. Results Overall, 85 (20%) dogs tested positive for at least one of the 6 pathogens investigated. The highest seroprevalence rate averaged over the 6-year period was 11.7% for D. immitis followed by 8.4% for E. canis and/or E. ewingii, 4.3% for A. phagocytophilum and/or A. platys, and 0.2% for B. burgdorferi. The co-exposure or co-infection was only detected in 3.8% of the dog population. Conclusions Together, opportunistic testing of dogs presenting for elective surgical procedures may provide an effective way of assessing seroprevalence and/or risk factors for common vector-borne diseases within a geographic region of concern.

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“…Prevalence of vector-borne infections also are likely to be affected by the age of the dogs tested, lifestyle and tick exposure risk, and overall health status, but that information was not available for inclusion in the analysis. In addition, although the assays used were designed to detect antibodies to Bb and Anaplasma spp., these tests may perform differently when used to test dogs infected with newly emergent, related organisms or those infected with agents undergoing antigenic variation [57,58].…”

Section: Discussionmentioning

confidence: 99%

Trends in canine seroprevalence to Borrelia burgdorferi and Anaplasma spp. in the eastern USA, 2010–2017

et al. 2019

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Background Borrelia burgdorferi and Anaplasma phagocytophilum are tick-borne infections transmitted by Ixodes scapularis in the eastern USA; both agents cause disease in dogs and people. To characterize changes in seroprevalence over time, Cochran Armitage trend tests were used to evaluate percent positive test results for antibodies to B. burgdorferi and Anaplasma spp. in approximately 20 million canine tests from 2010–2017 in 25 states and 905 counties in the eastern USA. Results A significant decreasing trend in seroprevalence to B. burgdorferi was evident in eight states along the mid-Atlantic coast from Virginia to New Hampshire, and in Wisconsin. In contrast, a continued increasing trend was evident in five northeastern and Midwestern states where Lyme borreliosis is endemic or emerging, as well as in three southern states where endemicity has not yet been widely established. Similarly, seroprevalence to Anaplasma spp. showed a significant, although smaller, decreasing trend in five states along the mid-Atlantic coast from Virginia to Connecticut and Rhode Island, as well as in Minnesota and Wisconsin in the Midwest; despite the fact that those trends were significant they were weak. However, a strong increasing trend was evident in Massachusetts and three states in northern New England as well as in Pennsylvania. Conclusions As expected, seroprevalence continued to increase in regions where Lyme borreliosis and anaplasmosis are more newly endemic. However, the declining seroprevalence evident in other areas was not anticipated. Although the reasons for the decreasing trends are not clear, our finding may reflect shifting ecologic factors that have resulted in decreased infection risk or the combined positive influence of canine vaccination, tick control, and routine testing of dogs in regions where these infections have long been endemic. Analysis of trends in canine test results for tick-borne infections continues to be a valuable tool to understand relative geographical and temporal risk for these zoonotic agents.

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Antibodies to Borrelia turicatae in Experimentally Infected Dogs Cross-React with Borrelia burgdorferi Serologic Assays

Cited by 19 publications

References 27 publications

First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe

First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe

Serological prevalence of six vector-borne pathogens in dogs presented for elective ovariohysterectomy or castration in the South central region of Texas

Trends in canine seroprevalence to Borrelia burgdorferi and Anaplasma spp. in the eastern USA, 2010–2017

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