Antibodies to a continuous region at residues 38-54 of hen egg white lysozyme found in a small fraction of anti-hen egg white lysozyme antibodies

Tsutsumi, Yutaka; Hirayama, Atsushi; Fujio, Hajime; Amano, Tsunehisa

doi:10.1021/bi00552a031

Cited by 29 publications

(5 citation statements)

References 21 publications

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“…All of these mouse strains are high responders to HEL and indeed anti-HEL antibodies could easily be isolated by HEL coupled to Sepharose CL-4B. However, in sharp contrast to our earlier studies in rabbits (8,9,23,32), sheep (22,32), and guinea pigs (10, 24), we have so far not succeeded in isolating HEL-region-specific antibodies by utilizing peptide (M.W. 4,000-5,000) affinity chromatography, presumably because of the low affinities of the peptide-antibody reactions.…”

Section: Discussioncontrasting

confidence: 75%

Suppression of Hen Egg‐White Lysozyme (HEL)‐Specific Delayed‐Type Hypersensitivity Responses in Mice by Suppressor T Cells after Neonatal Administration of Anti‐Idiotypic Antibodies

Semma

Amano

Fujio

et al. 1981

Microbiology and Immunology

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Anti-idiotypic rabbit antiserum (anti-Id) directed to the idiotypes of anti-hen egg-white lysozyme (HEL) antibody from a single C3H mouse (No.2) was shown to be capable of recognizing only a fraction of the anti-HEL antibody populations produced by other C3H mice. Experiments were performed to examine the effect of this particular anti-Id on the delayed-type hypersensitivity (DTH) response specific for the same protein antigen. A group of 60-day-old C3H mice which had been administered anti-Id within 24 hr after birth were tested for HEL-DTH response. The results indicated that the DTH response was completely suppressed by the anti-Id treatment. The inhibition of DTH reactivity is due to active suppression and involves the generation of suppressor T cells. Thus, the suppression induced with a single injection of anti-Id was transferable with both spleen cells and thymocytes from mice that received anti-Id. These suppressor cells are T cells since their ability to suppress DTH is completely abrogated by treatment in vitro with anti-Thy 1.2 serum and complement.Every immunoglobulin (Ig) variable region has, as well as antibody activity, an antigenicity which stems from its own unique structure. The individual anti-

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Section: Discussioncontrasting

confidence: 75%

Suppression of Hen Egg‐White Lysozyme (HEL)‐Specific Delayed‐Type Hypersensitivity Responses in Mice by Suppressor T Cells after Neonatal Administration of Anti‐Idiotypic Antibodies

Semma

Amano

Fujio

et al. 1981

Microbiology and Immunology

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“…The three discontinuous lysozyme sites account for more than 98% of anti-lysozyme antibodies raised in several species (Atassi, 1978;Atassi, 1979). A possible minor determinant, accounting for less than 1% of the total antibody, has also been identified (Takagaki et al, 1980). The participation of the surface-simulation sites in T cell recognition, however, has not previously been investigated.…”

Section: Discussionmentioning

confidence: 99%

Iii. Recognition of the ‘Surface‐simulation’ Synthetic Antigenic Sties

Bixler

Atassi

1984

Int J Immunogenetics

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SUMMARY In previous studies from this laboratory the antigenic sites of lysozyme were found to be composed of spatially adjacent surface residues that are mostly distant in sequence (i.e. discontinuous sites). For synthetic mimicking of the sites, we introduced the concept of ‘surface‐simulation’ synthesis by which the binding site residues are linked directly via peptide bonds with appropriate spacing and directionality into a single peptide which does not exist in the protein but mimics a surface region of it. In the present report T cell recognition of the surface‐simulation synthetic antigenic sites has been explored in a mouse strain, B10.BR, that is a high responder to lysozyme. The discontinuous antigenic sites of lysozyme also had the capacity to stimulate proliferation of T cells driven by native lysozyme in long‐term cultures. Thus, in addition to the four continuous T sites that we have recently reported, T cell recognition of lysozyme also involves discontinuous sites. This is the first clear demonstration that, contrary to a long‐held impression, T cell recognition is not restricted only to sequence features, but can also be directed to protein discontinuous surface areas of high conformational dependency.

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“…Also trace amounts of antibodies have been reported [92] to a continuous region of hen egg-white lysozyme (residues 25-54) that account for about 0.10-1% of the total antilysozyme antibodies. In very recent studies [lo -121, employing the overlapping synthetic peptide approach, peptides that were seemingly inactive in binding of '251-labelled anti-Hb antibodies in a titration assay were further studied by attempting to isolate, from adsorbents of these peptides, antibodies specific for Hb.…”

Section: Level Of Responses To Regions Outside the Antigenic Sitesmentioning

confidence: 99%

Antigenic structures of proteins. Their determination has revealed important aspects of immune recognition and generated strategies for synthetic mimicking of protein binding sites

Atassi

1984

Eur J Biochem

237

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Studies in this laboratory have resulted in the delineation and synthetic verification of several complete protein antigenic structures that are recognized by antibodies. More recently, for the first time, the full profiles of the sites that are recognized by T cells have been localized and confirmed by synthesis for two proteins, myoglobin and lysozyme. These have thus far constituted the only complete antigenic structures to be determined. The availability of these antigenic structures has enabled us to investigate in detail the molecular and cellular parameters responsible for immune recognition, responses to, and control and regulation of these responses to protein antigens at the molecular and submolecular levels. Moreover, these investigations have afforded general strategies for the synthetic mimicking of not only antigenic sites, but also protein binding sites involved in other biological activities.

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Antibodies to a continuous region at residues 38-54 of hen egg white lysozyme found in a small fraction of anti-hen egg white lysozyme antibodies

Cited by 29 publications

References 21 publications

Suppression of Hen Egg‐White Lysozyme (HEL)‐Specific Delayed‐Type Hypersensitivity Responses in Mice by Suppressor T Cells after Neonatal Administration of Anti‐Idiotypic Antibodies

Suppression of Hen Egg‐White Lysozyme (HEL)‐Specific Delayed‐Type Hypersensitivity Responses in Mice by Suppressor T Cells after Neonatal Administration of Anti‐Idiotypic Antibodies

Iii. Recognition of the ‘Surface‐simulation’ Synthetic Antigenic Sties

Antigenic structures of proteins. Their determination has revealed important aspects of immune recognition and generated strategies for synthetic mimicking of protein binding sites

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