Antibodies reactivity in humanSchistosoma mansonidisease: detection and functional activity

Thermostability of heterophile antibodies from human sera infected with Schistosoma mansoni and geo-helminths: an immuno-metric statistical analysis

“…As can be seen in the literature, the phenomenom of antibody in human sera which lyses sheep erythrocytes in the presence of complements in sera of schistosomotic children and adults has been insufficiently investigated up to now 2,9 . It has been reported that in the earlier stages of immune response anti-SRBC antibodies appeared as a thermolabile immune lysin, and finally as a thermostabile one 22,34 .…”

“…It has been reported that in the earlier stages of immune response anti-SRBC antibodies appeared as a thermolabile immune lysin, and finally as a thermostabile one 22,34 . Then, it has been noticed that this antibody activity (hemolysins) was rare in normal sera after inactivation at 56 o C 34 , and these criteria have been employed to distinguish normal from induced or "specific" antibody 2,9,22 . Previous studies indicated that in schistosomiasis the amount of anti-SRBC antibody differs from control individuals 3,9 .…”

“…2) Previous observations on schistosomotic adult individuals in a kinetic study showed that three patterns could be observed after heating of the sera, namely L4, R4 and R60 to be described bellow 2,9 : a decrease of more than 70% of hemolysin activity after heating for four minutes at 56 o C was observed in the first group (L4 serum type). The second group (R4 serum type) did not alter after heating for four minutes, but a sharp decrease was observed after 30 minutes, and following 60 minutes a loss of more than 70% of the hemolysin activity was also observed.…”

“…Sera were collected and used immediately. In order to make valid comparisons, the sera of schistosomotic individuals and controls were studied for the presence of lysins (naturally occurring complement activating macroglobulin antibodies) for SRBC 2,9 , in the following way: the natural hemolysin activity in each serum was assayed by mixing 1 mL of 1/20 human serum with 0.5 mL of a 0.8% SRBC suspension, which is spectrophotometrically standardized, and 2.0 mL of 1/400 guinea-pig serum (complement source). The mixture was incubated at 37 o C for 30 minutes.…”

“…Data analysis was certainly the basic part of group arrangement, as exemplified in a previous study with adult schistosomotic patients, the presentation displayed being important for functional antibody data visualization 2 . For example, we can compare, by using small samples, groups of individuals with some immunoparasitological characteristics by means of numerical values.…”