Antibodies provoked by the transfusion of biotin‐labeled red cells

Cordle, Delores G.; Strauss, Ronald G.; Lankford, Gary L.; Mock, Donald M.

doi:10.1046/j.1537-2995.1999.39101065.x

Cited by 40 publications

(55 citation statements)

References 7 publications

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“…Indeed, in one of our studies, antibodies to BioRBC developed in 3 of 20 adults transfused with autologous BioRBC [87]. To date a total of 4 adult study subjects have developed antibodies to BioRBC 3 to 4 months post-transfusion [57, 88]. None had evidence of hemolysis, change in the BioRBC survival, abnormalities of their peripheral blood count or RBC or reticulocyte indices, or untoward clinical signs or symptoms.…”

Section: Safety Of the Biotin Methodsmentioning

confidence: 99%

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Measurement of Posttransfusion Red Cell Survival With the Biotin Label

Mock

Widness

Veng‐Pedersen

et al. 2014

Transfusion Medicine Reviews

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The goal of this review is to summarize and critically assess information concerning the biotin method to label red blood cells (RBC) for use in studies of RBC and transfusion biology — information that will prove useful to a broad audience of clinicians and scientists. A review of RBC biology, with emphasis on RBC senescence and in vivo survival is included, followed by an analysis of the advantages and disadvantages of biotin labeled RBC (BioRBC) for measuring circulating RBC volume, post-transfusion RBC recovery, RBC lifespan, and RBC age-dependent properties. The advantages of BioRBC over 51Cr RBC labeling, the current reference method, are discussed. Because the biotin method is straightforward and robust, including the ability to follow the entire lifespans of multiple RBC populations concurrently in the same subject, BioRBC offers distinct advantages for studying RBC biology and physiology, particularly RBC survival. The method for biotin labeling, validation of the method, and application of BioRBCs to studies of sickle cell disease, diabetes, and anemia of prematurity are reviewed. Studies documenting the safe use of BioRBC are reviewed; unanswered questions requiring future studies, remaining concerns, and regulatory barriers to broader application of BioRBC including adoption as a new reference method are also presented.

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Section: Safety Of the Biotin Methodsmentioning

confidence: 99%

“…In addition, the best method for detecting antibodies to BioRBC has yet to be determined. Our groups initially used an assay for BioRBC antibodies based on agglutination in test tubes [88]. However, we have recently developed a more sensitive anti-IgG antibody method using gel cards (manuscript in preparation).…”

Section: Safety Of the Biotin Methodsmentioning

confidence: 99%

Measurement of Posttransfusion Red Cell Survival With the Biotin Label

Mock

Widness

Veng‐Pedersen

et al. 2014

Transfusion Medicine Reviews

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“…The major potential disadvantage of the biotin label is the development of antibodies against biotinylated RBC. In one report [23] transient antibodies were detected using an agglutination technique in 3 of 20 subjects that were given biotin-labeled RBC. None of these antibodies appeared to have an effect on RBC lifespan, and none could be detected after 1 year.…”

Section: Biotinmentioning

confidence: 99%

Measurement of Red Cell Lifespan and Aging

Franco

2012

Transfus Med Hemother

178

128

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The measurement of red blood cell (RBC) survival has a long history, and a wide variety of methods have been utilized for this purpose. Current methods are of 2 types. First, those that label a representative sample of RBCs of all ages from the blood and then measure their rate of disappearance upon reinfusion. This category includes the ⁵¹Cr and biotin labels. Second, those that use a metabolic precursor or product to determine the turnover of hemoglobin. Examples of these are carbon monoxide production and incorporation of labeled glycine. Recent studies with the covalent, nonradioactive biotin label show its unique suitability for both the accurate measurement of red cell survival and the determination of changes in red cell properties as they age in vivo.

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“…Biotinylation of erythrocytes is also readily achieved, and biotinylated erythrocytes can be safely reinfused into humans (40)(41)(42). This procedure would allow specific conjugation and multimerization of biotinylated viral receptors on the erythrocyte surface by using avidin.…”

Section: Discussionmentioning

confidence: 99%

The erythrocyte viral trap: Transgenic expression of viral receptor on erythrocytes attenuates coxsackievirus B infection

Asher

Cerny²,

Finberg³

2005

Proc. Natl. Acad. Sci. U.S.A.

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Viruses rely on attachment to specific cell surface receptors to infect host cells. Selective expression of viral receptors has the potential to attenuate infection of susceptible tissues by redirecting virus to cells that cannot support viral replication. We propose that erythrocytes are an ideal instrument for this strategy, because they are present in vast numbers, permeate every organ, and cannot serve as hosts for viral propagation. To test this hypothesis, we generated a transgenic mouse, termed globin transcription factor 1 (GATA1)-coxsackie and adenovirus receptor (CAR), that expressed the CAR on erythrocytes. Coxsackievirus group B (CVB) adhered to the surface of CAR-expressing erythrocytes and was rendered noninfectious. Upon infection with CVB, GATA1-CAR mice had diminished viremia and reduced viral replication in heart, brain, and liver. Furthermore, when faced with a CVB challenge that was lethal to WT littermates, the survival of GATA1-CAR mice was prolonged, and their ultimate mortality was reduced. The GATA1-CAR mouse model presented here demonstrates that erythrocyte expression of CAR limits CVB pathogenesis. Erythrocytes also may be coated with a variety of receptors by nontransgenic methods, making this a very flexible model for the treatment of infectious diseases in humans.coxsackie and adenovirus receptor ͉ virus receptor V iruses infect cells through attachment to specific host cell membrane receptors. These receptors mediate adhesion of the virus and facilitate its entry into the cell. The expression pattern of the specific receptors for a virus is thus a major determinant of viral tropism. Viruses cannot attack cells that do not bear the appropriate receptors, but they will attempt to infect cells that do, even if those cells are not suitable for viral propagation. If the receptor-expressing cell cannot support replication of the virus, then the virus will spend itself fruitlessly in an attempt to infect it. This idea can be advantageously applied by using selective expression of viral receptors to redirect virus to nonproductive cells, thus protecting susceptible tissues. We propose that erythrocytes are the ideal instrument for this strategy, because these cells are present in vast numbers, permeate every organ, are easily manipulated, are relatively disposable, and cannot serve as hosts for viral replication.Erythrocytes are simultaneously the most numerous and the simplest cells in the body. In their mature form, they lack the nuclei and organelles required to replicate nucleic acids and elaborate proteins. Because viruses depend on the use of the host cell machinery to replicate, erythrocytes are invulnerable to viral infection. The redirection of virus to erythrocytes has the potential to attenuate infection by leading virions to a dead end, leaving fewer infectious particles free to invade susceptible tissues. We hypothesized that the transgenic expression of viral receptor on erythrocytes would overwhelm the virus with decoy targets and thereby limit pathogenesis. Coxsackievirus ...

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Antibodies provoked by the transfusion of biotin‐labeled red cells

Cited by 40 publications

References 7 publications

Measurement of Posttransfusion Red Cell Survival With the Biotin Label

Measurement of Posttransfusion Red Cell Survival With the Biotin Label

Measurement of Red Cell Lifespan and Aging

The erythrocyte viral trap: Transgenic expression of viral receptor on erythrocytes attenuates coxsackievirus B infection

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