Antibodies in the serum of golden hamsters experimentally infected with the intestinal trematode Echinostoma caproni

Abstract

The serum antibody response in golden hamsters (Mesocricetus auratus) infected with the intestinal trematode Echinostoma caproni was examined with ELISA, SDS-PAGE and Western blot, and IFAT techniques. All methods showed that the hamsters responded slowly but developed a clear positive humoral response to the infection. In most hamsters, an antibody response to infection could not be detected earlier than 11–13 weeks after infection with 6 or 25 metacercariae, and responses were weak when compared to previous results from mice infected with the same parasite. IFAT with positive hamster sera on live juvenile E. caproni showed only fluorescence at the posterior tip, which is a different pattern from that seen using sera from infected mice, indicating a different response to antigens on the juvenile parasites by these two hosts. The results are discussed in relation to the limited selfcure and development of resistance which is observed in golden hamsters infected with E. caproni.

Kinetics of Echinostoma Caproni (Trematoda: Echinostomatidae) Antigens in Feces and Serum of Experimentally Infected Hamsters and Rats

“…Our results show that E. caproni evokes significant systemic antibody responses in hamsters from 35 to 42 DPI. This is in contrast to the results of Simonsen et al (1991). They reported that hamsters develop slow and weak humoral immune responses to E. caproni infections, and only low levels of antibodies were detected at 77-91 DPI using crude adult worms as antigen.…”

Antibody Responses in Sera of Different Mouse Strains Experimentally Infected with Neodiplostomum seoulense

“…We could find that specific IgG antibody responses against N. seoulense infection progressively increased and reached a maximum at day 28 PI in 3 strains of mice. This agrees to the previous studies on the antibody responses in mice infected with E. caproni [11,17,22]. From our results, IgG titers of both ICR and BALB/c mice were remarkably higher than in C3H mice, and the correlation between the IgG titers and worm expulsion was high and many antigenic band profiles were observed in western blot data.…”

Development of an Antibody-Based Capture Enzyme-Linked Immunosorbent Assay for Detecting Echinostoma Caproni (Trematoda) in Experimentally Infected Rats: Kinetics of Coproantigen Excretion

“…These studies have demonstrated that anti-E. caproni immunoglobulins can be detected in experimentally infected mice by 14 and 8 DPI depending on the antigen used (Agger et al, 1993;Graczyk andFried, 1994, 1995). Moreover, antibody detection methods have major limitations, i.e., the presence of antibodies indicates previous exposures rather than active infections, and considerably different patterns of anti-E. caproni antibody titers have been observed depending on the host species (Simonsen et al, 1991;Toledo et al, 2003). In the current study, detectable levels of antibodies were obtained only from 42-49 DPI, when the animals were free of parasites by parasitological criteria.…”

“…However, only a limited number of studies have considered this topic, and only indirect ELISA methods have been developed (Simonsen et al, 1991;Agger et al, 1993;Graczyk andFried, 1994, 1995;Toledo et al, 2003). These studies have demonstrated that anti-E. caproni immunoglobulins can be detected in experimentally infected mice by 14 and 8 DPI depending on the antigen used (Agger et al, 1993;Graczyk andFried, 1994, 1995).…”

“…However, the application of serologic diagnosis has major limitations, particularly in laboratory animals. For example, antibody titers persist after worm loss and also depend on the host species (Simonsen et al, 1991;Toledo et al, 2003). Moreover, animal handling to obtain a sufficient amount of serum samples is often difficult.…”