Antibodies against swine influenza virus neutralize the pandemic influenza virus A/H1N1

Tsukamoto,

doi:10.3892/mmr.2011.410

Cited by 22 publications

(16 citation statements)

References 6 publications

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“…However, in some cases, for example in the case of novel or unknown pathogens such as pandemic influenza, immediate protection may be desired. Tsukamoto et al (2011) reported the production of IgY against the pandemic influenza virus A/H1N1 2009, using a swine influenza virus vaccine strain. The resulting antibodies had strong cross reactivity to both the swine and human strains of the virus and were capable of neutralizing the A/H1N1 virus in vitro.…”

Section: Igy Use In Human Medicinementioning

confidence: 99%

Egg Yolk Antibodies for Passive Immunity

Kovacs-Nolan¹,

Mine

2012

Annu. Rev. Food Sci. Technol.

154

140

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The avian egg contains all of the necessary nutrients and growth factors required for the developing embryo, including antibodies that are transported from the blood of the hen into the egg yolk to provide immunity to the chick. Since the discovery of egg yolk antibodies, now called immunoglobulin Y (IgY), in the late 1800s, this process has been harnessed to produce antigen-specific yolk antibodies for numerous applications in the medical and research fields, including in areas such as diagnostics and proteomics. However, one of the most valuable and promising areas of IgY research is its use for passive immunization to treat and prevent human and animal diseases. The following review covers the key features and advantages of IgY and the production and purification of IgY from the egg yolk, as well as highlights some of the most promising applications of egg yolk antibodies in human and veterinary medicine.

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Section: Igy Use In Human Medicinementioning

confidence: 99%

Egg Yolk Antibodies for Passive Immunity

Kovacs-Nolan¹,

Mine

2012

Annu. Rev. Food Sci. Technol.

154

140

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“…The antibodies were derived from ostrich eggs immunized with a swine influenza virus vaccine strain. 95 The quantity of the IgY produced was very large and was found to have strong crossreactivity with pandemic influenza H1N1 and swine influenza virus as shown by ELISA and immunocytochemistry. Ostrich IgY antibodies were found to inhibit the hemaggregation activity of erythrocytes that was induced by pandemic influenza A/H1N1 virus.…”

Section: Advantages Of Passive Immunization With Igymentioning

confidence: 99%

IgY antibodies for the immunoprophylaxis and therapy of respiratory infections

Abbas

El‐Kafrawy

Sohrab

et al. 2018

Human Vaccines & Immunotherapeutics

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Emergence of drug resistance among the causative organisms for respiratory tract infections represents a critical challenge to the global health care community. Further, although vaccination can prevent disease, vaccine development is impeded by several factors. Therefore, novel approaches to treat and manage respiratory infections are urgently needed. Passive immunization represents a possible alternative to meet this need. Immunoglobulin Y antibodies (IgYs) from the yolk of chicken eggs have previously been used against bacterial and viral infections in human and animals. Their advantages include lack of reaction with mammalian Fc receptors, low production cost, and ease of extraction. Compared to mammalian IgGs, they have higher target specificity and greater binding avidity. They also possess remarkable pathogen-neutralizing activity in the respiratory tract and lungs. In this review, we provide an overview of avian IgYs and describe their potential therapeutic applications for the prevention and treatment of respiratory infections.

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“…Antibody titers of the obtained antiserum were measured by an enzyme-linked immunosorbent assay (ELISA). Furthermore, the neutralizing activity against avian influenza virus was scored by a hemagglutination inhibition (HI) test using erythrocytes [20].…”

Section: Generation Of Antisera Against A/h5n1 Virusmentioning

confidence: 99%

“…Based on our previous papers, binding ability of the chicken antisera to the viral antigen was measured by ELISA [20]. Each well of 96-well ELISA plates (Sumitomo Bakelite, Japan) was coated with antigens of pandemic influenza virus A/H1N1 (A/California/9/2009 (H1N1) pdm09) or avian A/H5N1 virus in phosphate-buffered saline (PBS), and the ELISA plate was stored overnight at 4˚C.…”

Section: Elisamentioning

confidence: 99%

Prevention of Highly Pathogenic Avian Influenza A/H5N1 Infection by Passive Immunotherapy Using Antiserum

Adachi¹,

Suryaman²,

Soejoedono³

et al. 2018

AiM

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The rapid epidemic of highly pathogenic A/H5N1 avian influenza virus by transmission from poultry to humans triggered global unrest in the pandemic of novel influenza. If a human trophic strain of avian influenza viruses replicates in livestock including pigs and chickens, it may have high infectivity and pathogenicity to humans. The most effective method of reducing the outbreaks of influenza would be prophylaxis with an effective vaccine as well as anti-viral drugs including Oseltamivir and Zanamivir hydrate. In this study, chicken antiserum against A/H5N1 virus was produced: the antisera from immunized adult chicken had a strong binding activity to A/H5N1 viral antigens by ELISA. Furthermore, the antiserum strongly inhibited hemaggregation of erythrocytes and cytopathic effects in MDCK cells, indicating a strong neutralization activity against A/H5N1 infections. Interestingly, the mortality rate of chicks inoculated with A/H5N1 virus was dramatically decreased with the antiserum injection. These results suggest that antiserum may be a potentially effective protective and therapeutic modality for A/H5N1 infection.

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Antibodies against swine influenza virus neutralize the pandemic influenza virus A/H1N1

Cited by 22 publications

References 6 publications

Egg Yolk Antibodies for Passive Immunity

Egg Yolk Antibodies for Passive Immunity

IgY antibodies for the immunoprophylaxis and therapy of respiratory infections

Prevention of Highly Pathogenic Avian Influenza A/H5N1 Infection by Passive Immunotherapy Using Antiserum

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