Antibodies Against Sarcocystis neurona , Neospora spp., and Toxoplasma gondii in Horses and Mules From the Northern Pantanal Wetland of Brazil

Borges, Alice Mamede Costa Marques; Yeargan, Michelle R.; Silva, Lucas Gaíva; Taques, Ísis Indaiara Gonçalves Granjeiro; Howe, Daniel K.; Aguiar, Daniel Moura de

doi:10.1016/j.jevs.2017.04.007

Cited by 8 publications

(10 citation statements)

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“…Embora se tenha utilizado antígenos de Neospora caninum, não é possível determinar a provável espécie envolvida devido a reatividade cruzada ocasionada por N. hughesi (Marsh et al 1998, Gondim et al 2009. No Brasil, além de não haver registros oficiais de infecção por N. hughesi, Borges et al (2017) relataram maior reatividade a antígenos brutos de N. caninum por WB do que quando utilizado antígenos de N. hughesi.…”

Section: Discussionunclassified

Fatores de risco associados à prevalência de anticorpos anti-Sarcocystis neurona, Neospora spp. e Toxoplasma gondii em equinos de Roraima, Amazônia

et al. 2018

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RESUMO: Amostras de sangue de 303 equinos provenientes de 56 propriedades do município de Rorainópolis, Roraima, foram avaliadas por Reação de Imunofluorescência Indireta (RIF) para pesquisa de anticorpos contra Sarcocystis neurona, Toxoplasma gondii e Neospora spp. Algumas amostras de soros positivos para Sarcocystis spp. foram avaliadas pelo Western Blotting (WB) utilizando antígenos crus de S. neurona. A partir dos resultados sorológicos, possíveis fatores de risco foram avaliados frente a variáveis individuais e de propriedade. A prevalência de anticorpos anti-Sarcocystis spp. foi estimada em 43,2% (37,6-49,0%), anti-Neospora sp. em 26,7% (21,9-32,2%) e anti-T. gondii de 18,5% (14,3-23,4%). Quatorze amostras (14/15) testadas por WB resultaram positivas para antígenos de S. neurona. Das propriedades, 76,8% (43/56) apresentaram pelo menos um equino positivo para Sarcocystis spp.; 69,6% (39/56) para Neospora spp. e 55,4% (31/56) para T. gondii. Dos equinos, 13 (4,3%) apresentarem anticorpos para os três agentes, 50 (16,5%) para Sarcocystis spp. e Neospora spp., 10 (3,3%) para Neospora spp. e T. gondii, e oito (2,6%) para Sarcocystis spp. e T. gondii. As variáveis associadas (P≤0,05) à presença de anticorpos foram: para Neospora spp. não pastejar em áreas alugadas, ausência de assistência veterinária na propriedade, sexo masculino, não estabular animais e plantel equino acima de 5 animais; enquanto para T. gondii foram o contato com felinos, animais da raça lavradeiro, animal estabulado, criação de bovinos na propriedade e plantel equino acima de 5 animais. Não houveram variáveis associadas a presença de anticorpos contra S. neurona. Relata-se no presente estudo a primeira detecção de anticorpos anti-S. neurona, Neospora spp. e T. gondii em equinos do estado de Roraima, localizado na Amazônia Setentrional Brasileira, ressaltando para a elevada frequência de fazendas com equinos soropositivos.

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Section: Discussionunclassified

Fatores de risco associados à prevalência de anticorpos anti-Sarcocystis neurona, Neospora spp. e Toxoplasma gondii em equinos de Roraima, Amazônia

et al. 2018

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“…The presence of anti-N. caninum antibodies has also been reported in different animals, including cattle and dogs, in the state of Mato Grosso (Benetti et al, 2008;Benetti et al, 2009;Boa Sorte et al, 2015;Melo et al, 2012;Rodrigues et al, 2016). In the Pantanal wetlands, equids have been found seropositive for both N. caninum and T. gondii (Borges et al, 2017). In addition, a serological survey of free living jaguars (Panthera onca) in this region revealed high prevalence rates of antibodies to both parasites (Onuma et al, 2014).…”

Section: Introductionmentioning

confidence: 75%

“…The association between home-cooked diets and the seropositivity to the protozoan T. gondii is relevant, given that infections by this agent may also be acquired by ingesting tissue-inhabiting stages of the parasite and that any animal tissues, especially undercooked meat containing cysts with bradyzoites, may be a source of infection for dogs (Hill et al, 2005). Borges et al (2017), who evaluated equids in this region, found a large number of seropositive animals. In this context, the possible contribution of domestic or wild felids should be considered, since the presence of cats in urban areas and on farms is common; in fact, 50% of the dogs in our study have contact with cats.…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Neospora caninum and Toxoplasma gondii antibodies in dogs in the municipality of Poconé, state of Mato Grosso, Brazil

Melo¹,

Grangeiro²,

Aguiar³

2019

Braz. J. Vet. Res. Anim. Sci.

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Tis study evaluated the presence of Neospora caninum and Toxoplasma gondii antibodies in 320 domestic dogs living in urban and rural areas of the municipality of Poconé, located in the Pantanal wetlands of the state of Mato Grosso, Brazil. Anti-N. caninum (cut-of point 50) and anti-T. gondii (cut-of point 16) antibodies were detected by means of immunofuorescence assays (IFA), using NC-Bahia and RH strains as antigens, respectively. Anti-N. caninum antibodies were detected in 69 (21.56%; 95% CI: 17.27%-26.56%) dogs, 31 (44.93%) of which lived in urban areas and 38 (55.07%) in rural areas, and endpoint titers ranged from 50 to 3200. Anti-T. gondii antibodies were found in 132 (41.25%; 95% CI: 35.84%-46.87%) dogs, 58 (43.94%) from urban areas and 74 (56.06%) from rural areas, and endpoint titers ranged from 16 to 8192. A total of 33 dogs (10.3%) (12 urban and 21 rural animals) reacted to both agents (P <0.05). Tis study showed a higher prevalence of anti-T. gondii compared to anti-N. caninum antibodies in the dogs living in the Pantanal region, suggesting that both protozoans circulate in the studied region. Tis information is relevant, in view of its implications for animal and public health.

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“…As previously mentioned, the studies conducted by means of S. neurona ELISA and IFAT presented the possibility of detection of some false-positive results, due to horse antibodies against other Sarcocystis spp. In addition, the studies using combinations of tests or IB as confirmatory after a positive IFAT or ELISA result showed a lower number of "true positive" samples (Borges et al, 2017;Valença et al, 2019). A study performed in Argentina using IB revealed a twofold seropositivity rate (odds ratio 2.27) in horses with neurological signs, compared with horses without clinical signs (39.2% versus 22.1%, respectively), thus suggesting that S. neurona might be implicated in the occurrence of neurological disorders (Moré et al, 2014).…”

Section: Serological Studies On South American Horsesmentioning

confidence: 99%

“…Other equids from Brazil have also been tested for antibodies against S. neurona. In a study using 47 mule samples processed using IFAT (SN37R at 1:25 dilution), seropositivity of 17% was recorded (Borges et al, 2017).…”

Section: Serological Studies On South American Horsesmentioning

confidence: 99%

Sarcocystis neurona and related Sarcocystis spp. shed by opossums (Didelphis spp.) in South America

Gondim

Soares

Moré

et al. 2021

Rev. Bras. Parasitol. Vet.

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Protozoan parasites of the genus Sarcocystis are obligatory heteroxenous cyst-forming coccidia that infect a wide variety of animals and encompass approximately 200 described species. At least four Sarcocystis spp. (S. falcatula, S. neurona, S. lindsayi and S. speeri) use opossums (Didelphis spp.) as definitive hosts, and two of them, S. neurona and S. falcatula, are known to cause disease in horses and birds, respectively. Opossums are restricted to the Americas, but their distribution in the Americas is heterogeneous. Five Didelphis spp. are distributed in South America (D. aurita, D. albiventris, D. marsupialis, D. imperfecta and D. pernigra) whereas just one opossum species (D. virginiana) is found in North America. Studies conducted in the last decades show that Sarcocystis spp., derived from South American Didelphis spp., have biological and genetic differences in relation to Sarcocystis spp. shed by the North American opossum D. virginiana. The aim of this review was to address the peculiar scenario of Sarcocystis species shed by South American opossums, with a special focus on diagnosis, epidemiology, and animal infections, as well as the genetic characteristics of these parasites.

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Antibodies Against Sarcocystis neurona , Neospora spp., and Toxoplasma gondii in Horses and Mules From the Northern Pantanal Wetland of Brazil

Cited by 8 publications

References 34 publications

Fatores de risco associados à prevalência de anticorpos anti-Sarcocystis neurona, Neospora spp. e Toxoplasma gondii em equinos de Roraima, Amazônia

Fatores de risco associados à prevalência de anticorpos anti-Sarcocystis neurona, Neospora spp. e Toxoplasma gondii em equinos de Roraima, Amazônia

Prevalence of Neospora caninum and Toxoplasma gondii antibodies in dogs in the municipality of Poconé, state of Mato Grosso, Brazil

Sarcocystis neurona and related Sarcocystis spp. shed by opossums (Didelphis spp.) in South America

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