Antibodies against Mycobacterial Proteins as Biomarkers for HIV-Associated Smear-Negative Tuberculosis

Abstract

؊ (0% and 6%, respectively), and at moderate risk for TB development, i.e., U.S. HIV ؉ TST ؉ controls (14% and 10%, respectively). By contrast, more reactivity was detected in the S.A. HIV ؉ control group at higher risk for TB (25% and 45%, respectively). Our data hold promise that antibody detection against MPT51 and echA1 might have adjunctive value in the detection of HIV ؉ smear-negative TB and might reflect increasing Mycobacterium tuberculosis infection activity in asymptomatic HIV ؉ individuals.

Identification of Antibody Targets for Tuberculosis Serology using High-Density Nucleic Acid Programmable Protein Arrays

“…The cellular location for two of the proteins (Rv3405c and Rv3544c) has not been identi- fied. All of the four CFPs (Rv0054 (9, 10, 41), Rv0831c (9, 42), Rv2031c (9,10,43,44) and Rv0222 (45,46)), have already been reported in TB serology studies. Rv2031c (hspX) was also previously identified using a Mtb proteomic level microarray with printing of unpurified Mtb proteins generated in E. coli lysate (6).…”

Biomarcadores da tuberculose: uma revisão da literatura.

“…Como esperado, as respostas para esses Ag comparadas com as de outras populações em trabalhos prévios foram heterogêneas, até mesmo entre populações dentro da África. Outro ponto importante foi a confirmação de experiências anteriores (GUPTA et al, 2013;SIEV et al, 2014) que afirmam que a carga de CD4 não tem correlação clara com a resposta humoral nesses casos.…”

Serological Diagnostic Assays for HIV-Associated Tuberculosis in Sub-Saharan Africa?

“…Siev and colleagues evaluated serological responses to MPT51 and MS and two other immunodominant proteins (echA1 and the 38-kDa protein) in patients with HIV-associated sputum smearnegative TB and CD4 counts exceeding 400 cells/l (30). In this case-control study, positive antibody responses to MPT51 and echA1 were observed in more than one-half of the TB cases and in a minority of the control group subjects studied at a single time point.…”

“…In this issue of Clinical and Vaccine Immunology, Siev and colleagues present an evaluation of antibody responses to several immunodominant proteins of Mycobacterium tuberculosis in patients with HIV-associated TB in South Africa (30). Among the range of purified immunodominant antigens that have previously been evaluated for serological detection of M. tuberculosis (31), selected proteins which demonstrate greater potential utility as biomarkers of TB among HIV-infected patients have been studied by Siev and colleagues (32,33).…”

“…Many previous studies of serological responses to M. tuberculosis antigens have studied patients with sputum smear-positive pulmonary TB (31). In contrast, Siev and colleagues have examined serological responses among a diagnostically more challenging group, HIV-infected individuals who have sputum smear-negative disease (30). Furthermore, the study population was recruited in a setting in KwaZuluNatal, South Africa, where there is an extremely high burden of HIV-associated TB.…”