Antibodies against filamentous components in discrete cell types of the mouse retina

Drager, UC; Edwards, D L; Barnstable, C.J.

doi:10.1523/jneurosci.04-08-02025.1984

Cited by 212 publications

(115 citation statements)

References 51 publications

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“…In mouse retina, this antibody mainly labeled processes of horizontal cells and axons of ganglion cells (Dräger et al, 1984;Inoue et al, 2002). In zebrafish retina, strong labeling was observed in rod and cone photoreceptor outer segments (Adamus et al, 1988).…”

Section: Anti-sv2mentioning

confidence: 96%

Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish

Ricatti

Alfie

Lavoie

et al. 2008

Synapse

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Here we show a detailed immunocytochemical localization of two enzymes of the ENTPDase family in the retinal layers of two vertebrate species, namely, the mouse and the zebrafish. In the mouse retina, NTPDase2 was chiefly localized in Mü ller glia and ganglion cell processes. NTPDase1 was located on neurons as well, since it was expressed by horizontal and ganglion cell processes, suggesting that nucleotides such as ATP and ADP can be hydrolyzed at the surface of these cells. NTPDase1 was also detected in intraretinal blood vessels of the mouse. Regarding zebrafish, NTPDases1 and 2 seem to be differentially localized in horizontal cell processes, photoreceptor segments, and ganglion cell dendrites and axons, but absent from Mü ller glia. Moreover, NTPDases1 and 2 appear to be expressed within the germinal margin of the zebrafish retina that contains proliferative and differentiating cells. Retinal homogenates from both species exhibited ecto-ATPase activity which might be attributed at least to NTPDases1 and 2, whose expression is described in this report. Our results suggest a compartmentalized regulation of extracellular nucleotide/nucleoside concentration in the retinal layers, supporting a relevant role for extracellular nucleotide mediated-signaling in vertebrate retinas. Synapse 63:291-307,

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Section: Anti-sv2mentioning

confidence: 96%

Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish

Ricatti

Alfie

Lavoie

et al. 2008

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“…18 Expression of neurofilament proteins was detected by immunofluorescence staining of acetone-fixed cells as previously described, 13 using monoclonal antibody 7H11 that recognizes the 200 kDa neurofilament-protein. 35 …”

Section: Antigen Expressionmentioning

confidence: 99%

Hybrids of pluripotent and nullipotent human embryonal carcinoma cells: Partial retention of a pluripotent phenotype

et al. 2001

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To investigate whether the failure of human EC cells that do not differentiate is due to the loss of key differentiationpermissive functions or the acquisition of specific inhibitory functions, we tested the ability to differentiate of 2 hybrids produced between a relatively nullipotent human EC cell line, 2102Ep, and a pluripotent human EC cell line NTERA2. Both hybrids, which exhibited an EC phenotype, were able to differentiate readily in response to retinoic acid. Furthermore, 1 hybrid produced a well-differentiated xenograft tumor, which contained, like the NTERA2 tumors, glandular structures, loose mesenchymal tissues and nodules of cartilage, after injection into a SCID mouse. Thus, the failure of 2102Ep EC cells to differentiate is recessive and due to the loss of a key gene function or functions. Nevertheless, the hybrids differed from the pluripotent NTERA2 line by failing to differentiate in neurons, indicating that 2102Ep cells also had acquired a specific, dominantly-acting, inhibitory mutation specific to the neural lineage. Furthermore, the expression of collagen II by one hybrid before and after induction with retinoic suggested a propensity for spontaneous differentiation not evident in the parental NTERA2 cells. Thus, the mechanisms that restrict the differentiation capacity of the nullipotent 2102Ep line are complex and include both recessive and dominant acting factors.

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“…In addition, R5 staining of immunoblots was consistently much less intense than that of H5. The relative weakness of the R5 signal may explain the inability of Drager et al (1984) to detect an R5-reactive protein on immunoblots of rodent tissue. Three of the other mabs also recognized antigens on immunoblots.…”

Section: Identification Of Glial Antigensmentioning

confidence: 99%

“…3) Antibodies H28 and 29 were generated as in fusion 2, except that E l 0 hindbrain was used for suppression instead of E l 0 forebrain. 4) R5 was generated by Drager et al (1984), using murine retinal ganglion cells as immunogen. The RS-producing cell line was generously provided to us by Dr. Drager.…”

Section: Experimental Procedures Production Of Antibodiesmentioning

confidence: 99%

Developmentally regulated and spatially restricted antigens of radial glial cells

Herman

Victor

Sanes

1993

Developmental Dynamics

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Radial glial cells, present in many parts of the embryonic vertebrate central nervous system (CNS), have been implicated in the guidance of neuroblasts from the ventricular zone to their laminar destinations. Moreover, radial glial cells may be progenitors of some CNS neurons and glia. To gain new insight into the structure and development of these cells, we have generated and characterized a panel of monoclonal antibodies that recognize radial glial cells of the chick optic tectum. Mice were immunized with homogenates of embryonic day (E) 10 tectum, and antibodies were analyzed by immunofluorescence and immunoblotting. We describe here three pairs of antibodies. 1) H5 and a previously generated antibody, R5 (Drager et al., J. Neurosci. 42025, 1984), stain the whole extent of the radial glial cell from E7 to E20. In cultures prepared from El0 tecta, both stain a filamentous meshwork in glial cells but not in neurons. On immunoblots, both recognize a protein of -52 kD that is closely related (or identical) to vimentin. 2) H28 and H29 stain radial glia between E7 and E14, but not later. Moreover, H28 and H29 staining is markedly more intense in the ventricular and intermediate zones than in the laminae of the tectal plate. Both of these antibodies recognize an intracellular epitope in cultured glial cells and a protein of -35 kD on immunoblots. 3) H2 and H27 recognize antigens concentrated in the most superficial processes and endfeet of radial glia in late (E16-E20) embryos. They stain distinct structures in cultured glia, suggesting that they recognize distinct antigens. H27 recognizes a protein of -29 kD on immunoblots. Thus antibodies H5 and R5 are good markers of radial glial cells at all stages, whereas the others define antigens that are developmentally regulated and localized to discrete domains. Together, these antibodies can be used to study temporal and spatial specializations of radial glia. o 1993 %ley-Liss, Inc.

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Antibodies against filamentous components in discrete cell types of the mouse retina

Cited by 212 publications

References 51 publications

Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish

Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish

Hybrids of pluripotent and nullipotent human embryonal carcinoma cells: Partial retention of a pluripotent phenotype

Developmentally regulated and spatially restricted antigens of radial glial cells

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