Antibodies against a Leishmania infantum peroxiredoxin as a possible marker for diagnosis of visceral leishmaniasis and for monitoring the efficacy of treatment

Santarém, Nuno; Tomás, Ana M.; Ouaissi, Ali; Tavares, Joana; Ferreira, Nilza; Manso, Arêlo; Campino, Lenea; Correia, José Manuel; Cordeiro-da-Silva, Anabela

doi:10.1016/j.imlet.2005.04.006

Cited by 34 publications

(31 citation statements)

References 26 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, our results suggest that the dynamics of antibodies against recombinant proteins may be more useful than the dynamics of antibodies against CTLA for assessing clinical improvement after treatment, as described in human patients treated for visceral leishmaniasis. In accordance with our results, antibodies against the recombinant proteins rKMPII 25 and Li cTXNPx, a protein similar to TRYP, 26 decreased significantly after treatment of patients with visceral leishmaniasis, conversely to serologic results with crude Leishmania antigen.…”

Section: Discussionsupporting

confidence: 91%

Dynamics and Predictive Potential of Antibodies against Insect-Derived Recombinant Leishmania infantum Proteins during Chemotherapy of Naturally Infected Dogs

Todolí

Galindo²,

Gómez-Sebastián³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 91%

Dynamics and Predictive Potential of Antibodies against Insect-Derived Recombinant Leishmania infantum Proteins during Chemotherapy of Naturally Infected Dogs

Todolí

Galindo²,

Gómez-Sebastián³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

“…For comparison, the same cohort was tested for the presence of antibodies against SPLA, SALA, or LimTXNPx. The antigen concentration and serum dilution used in all ELISA assays were previously optimized (10,28,35). Therefore, we used 5 g/ml of each recombinant antigen and 10 g/ml of both Leishmania promastigote and amastigote soluble antigens as optimal concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, rK39 performs suboptimally in asymptomatic cases with proven infection (22). We have recently described the L. infantum cytosolic tryparedoxin peroxidase (LicTXNPx) antigen, a member of unique enzymatic Leishmania cascades for detoxification of peroxides (8), as a highly immunogenic probe during natural human or experimental canine infections (28,35). Our previous studies indicated that anti-LicTXNPx antibodies were present in both symptomatic and asymptomatic experimental canine infections, making this antigen a good candidate marker for both clinical and subclinical Leishmania infections (35).…”

mentioning

confidence: 99%

Application of an Improved Enzyme-Linked Immunosorbent Assay Method for Serological Diagnosis of Canine Leishmaniasis

et al. 2010

Self Cite

View full text Add to dashboard Cite

Accurate diagnosis of canine leishmaniasis (CanL) is essential toward a more efficient control of this zoonosis, but it remains problematic due to the high incidence of asymptomatic infections. In this study, we present data on the development of enzyme-linked immunosorbent assay (ELISA)-based techniques for the detection of antibodies against the recombinant protein Leishmania infantum cytosolic tryparedoxin peroxidase (LicTXNPx) and a comparison of the results with those employing soluble Leishmania antigens from promastigote or amastigote forms and the homologue recombinant protein L. infantum mitochondrial TXNPx (LimTXNPx). Moreover, we offer an evaluation of the diagnostic potential of rK39 for CanL in the Portuguese canine population and propose an improvement to the existing ELISA-based serological techniques by combining the LicTXNPx and rK39 antigens as a Leishmania antigen mixture (LAM). The data demonstrated that ELISAs based on soluble promastigote or amastigote antigens had generally higher levels of sensitivity for detection of antibodies in symptomatic or asymptomatic dogs than for detection of those against isolated recombinant proteins. Nevertheless, the specificities were found to be similar for all target antigens used. Importantly, the LAM-ELISA methodology improved the overall sensitivity, maintaining a high overall level of specificity. In addition, it was demonstrated that the detection of anti-LAM IgG2 can increase the accuracy of the serological diagnosis. Overall, the obtained results showed that the strategy of combining two well-defined

show abstract

“…Tryparedoxin peroxidase belongs to a family of proteins called peroxiredoxins, which are associated with parasite virulence and have a high antigenicity during the active disease. Previous studies have shown the antigenicity of peroxiredoxins in dogs and humans infected by Leishmania infantum or L. braziliensis (46,47). Khosravi et al showed that a realtime PCR using the tryparedoxin peroxidase gene was specific for CL diagnosis (48).…”

Section: Discussionmentioning

confidence: 99%

Proteins Selected in Leishmania (Viannia) braziliensis by an Immunoproteomic Approach with Potential Serodiagnosis Applications for Tegumentary Leishmaniasis

Duarte

Pimenta

Menezes‐Souza

et al. 2015

Clin Vaccine Immunol

View full text Add to dashboard Cite

The serodiagnosis of human tegumentary leishmaniasis (TL) presents some problems, such as the low level of antileishmanial antibodies found in most of the patients, as well as the cross-reactivity in subjects infected by other trypanosomatids. In the present study, an immunoproteomic approach was performed aimed at identification of antigens in total extracts of stationaryphase promastigote and amastigote-like forms of Leishmania (Viannia) braziliensis using sera from TL patients. With the purpose of reducing the cross-reactivity of the identified proteins, spots recognized by sera from TL patients, as well as those recognized by antibodies present in sera from noninfected patients living in areas where TL is endemic and sera from Chagas disease patients, were discarded. Two Leishmania hypothetical proteins and 18 proteins with known functions were identified as antigenic. The study was extended with some of them to validate the results of the immunoscreening. The coding regions of five of the characterized antigens (enolase, tryparedoxin peroxidase, eukaryotic initiation factor 5a, ␤-tubulin, and one of the hypothetical proteins) were cloned in a prokaryotic expression vector, and the corresponding recombinant proteins were purified and evaluated for the serodiagnosis of TL. The antigens presented sensitivity and specificity values ranging from 95.4 to 100% and 82.5 to 100%, respectively. As a comparative antigen, a preparation of Leishmania extract showed sensitivity and specificity values of 65.1 and 57.5%, respectively. The present study has enabled the identification of proteins able to be employed for the serodiagnosis of TL. L eishmaniasis consists of a spectrum of diseases caused by protozoan parasites of the genus Leishmania, which present high morbidity and mortality throughout the world (1). Approximately 350 million people in 98 countries are at risk of contracting the infection, while nearly 1.0 to 1.5 million cases of tegumentary leishmaniasis (TL) and 0.2 to 0.5 million cases of visceral leishmaniasis (VL) are registered annually (2). Although TL is not a fatal disease, it is endemic in more than 70 countries, and 90% of the cases have occurred in Afghanistan, Algeria, Brazil, Pakistan, Peru, Saudi Arabia, and Syria (3). The disease exhibits distinct clinical manifestations, such as cutaneous leishmaniasis (CL), diffuse cutaneous leishmaniasis (DCL), and mucosal leishmaniasis (ML) (4, 5). In Brazil, TL is caused mainly by infection with the species Leishmania (Viannia) braziliensis, Leishmania (V.) guyanensis, and Leishmania (Leishmania) amazonensis, although parasitological and molecular evidence has shown that L. braziliensis is the most important etiological agent of the disease (6, 7).At present, there is no gold standard test for TL diagnosis, and a combination of diagnostic methods is frequently needed to obtain more precise results (8). Parasitological diagnosis is definitive and consists of the microscopic examination of Giemsa-stained biopsy specimen smears, of histopathological examinati...

show abstract

Antibodies against a Leishmania infantum peroxiredoxin as a possible marker for diagnosis of visceral leishmaniasis and for monitoring the efficacy of treatment

Cited by 34 publications

References 26 publications

Dynamics and Predictive Potential of Antibodies against Insect-Derived Recombinant Leishmania infantum Proteins during Chemotherapy of Naturally Infected Dogs

Dynamics and Predictive Potential of Antibodies against Insect-Derived Recombinant Leishmania infantum Proteins during Chemotherapy of Naturally Infected Dogs

Application of an Improved Enzyme-Linked Immunosorbent Assay Method for Serological Diagnosis of Canine Leishmaniasis

Proteins Selected in Leishmania (Viannia) braziliensis by an Immunoproteomic Approach with Potential Serodiagnosis Applications for Tegumentary Leishmaniasis

Contact Info

Product

Resources

About