Antibiotics safety case studies: Hospitals

Khan, Faiz Ullah; Shah, Shahid; Abbas, Ghulam; Khan, Hafeez Ullah; Ahmad, Tawseef; Ullah, Wasim; Khan, Abdullah; Khan, Farman Ullah; Mallhi, Tauqeer Hussain; Khan, Yusra Habib; Fang, Yu

doi:10.1016/b978-0-323-98802-5.00003-0

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…The stub was gently tapped twice to remove the excess powder from the surface of the aluminum stub leaving a thin film of nanocrystals on the surface of the stub. The sample was gold coated using a sputter coater and representative sections were photographed at different magnifications to get clear images (Khan et al, 2020).…”

Section: Morphologymentioning

confidence: 99%

Untitled

2023

Pak. J. Pharm. Sci.,

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Indomethacin a Non-steroidal anti-inflammatory drug (NSAID) demonstrates low bioavailability. To counter the problem an attempt was made by preparing indomethacin nanocrystals in a micro-channel fluid reactor using different concentrations of the polymers. Indomethacin crystals were optimized by determining the particle size of 380 nm ± 5.0 with PDI of 0.29±0.05. The crystalline nature was confirmed with the help of Powder X-Ray diffraction and Differential scanning calorimetry. A high antisolvent volume (2.0/0.5ml/min), low inlet angle (10 °) and mixing time of 60 min with 15 min sonication at a high rate of stirring were the key parameters that produced Indomethacin nanocrystals with good particles size. A high anti-solvent volume (2.0/0.5ml/min), low inlet angle (10 °) and mixing time of 60 min with 15 min sonication at a high rate of stirring were the key parameters that produced Indomethacin nanocrystals with good particle size. Similarly, the polymeric combination of including Polyvinylpyrrolidone k-30 (1%)-Hydroxypropyl methyl cellulose-15cps (0.5%) and Sodium lauryl sulfate (0.5%) were the appropriate concentrations. The Transmission electron microscopy and Scanning electron microscopy studies demonstrated the homogeneity with a distinct crystalline structure. The stability study displayed stability at 4 o C and 25 o C. The nanocrystals exhibited a dissolution rate correlated to the marketed and unprocessed drugs.

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Section: Morphologymentioning

confidence: 99%

Untitled

2023

Pak. J. Pharm. Sci.,

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“…Genomic information is widely available to find pathogenic proteins apt for vaccine development. Recently, the combination of immunoinformatics with the subtractive proteomic technique has grown more appealing for the development of effective, affordable vaccines against a variety of infections (Khan et al, 2019). In the present study, subtractive proteomics filters were used to screen potential proteins for vaccine development from the complete proteome.…”

Section: Introductionmentioning

confidence: 99%

Integrated immunoinformatics and subtractive proteomics approach for multi-epitope vaccine designing to combat S. pneumoniae TIGR4

Ashgar¹,

Faidah²,

Bantun³

et al. 2023

Front. Mol. Biosci.

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Streptococcus pneumoniae is one of the major precarious pathogens accountable for over 1.2 million fatalities annually. The key drivers for pneumococcal vaccine development involve high morbidity and mortality in over one million cases, especially in very young children and the elderly. In this study, immunoinformatics was integrated with subtractive proteomics to find antigenic proteins for designing a multi-epitope vaccine against S. pneumoniae. As prospective vaccine targets, the developed pipeline identified two antigenic proteins, i.e., penicillin-binding protein and ATP synthase subunit. Several immunoinformatics and bioinformatics resources were used to forecast T- and B-cell epitopes from specific proteins. By employing a mixture of five cytotoxic T-cell lymphocytes, six helper T-cell lymphocytes, and seven linear B-cell lymphocyte epitopes, a 392 amino acid-long vaccine was designed. To enhance immune responses, the designed vaccine was coupled with a cholera enterotoxin subunit B adjuvant. The designed vaccine was highly antigenic, non-allergenic, and stable for human usage. The stability of the vaccine with toll-like receptor-4 was evaluated by molecular docking and molecular dynamic simulation. In addition, immunological simulation was performed to test its real-world potency. The vaccine codon was then cloned in silico. Overall, this study paves the way for the development of a multi-epitope S. pneumoniae vaccine under laboratory conditions. Furthermore, the current findings warrant for the experimental validation of the final multi-epitope vaccine construct to demonstrate its immunological reinforcing capability and clinical applicability.

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State-of-the-Art of Synthesized Exosomes and NPs-Based Biomimetic Nanoparticles for Wound Rehabilitation: A Review

Issaka

2023

Biomedical Materials & Devices

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Antibiotics safety case studies: Hospitals

Cited by 3 publications

References 20 publications

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Integrated immunoinformatics and subtractive proteomics approach for multi-epitope vaccine designing to combat S. pneumoniae TIGR4

State-of-the-Art of Synthesized Exosomes and NPs-Based Biomimetic Nanoparticles for Wound Rehabilitation: A Review

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