Antibiotics Reduce the Growth Rate and Differentiation of Embryonic Stem Cell Cultures

Cohen, Shahar; Samadikuchaksaraei, Alí; Polak, Julia M.; Bishop, Anne E.

doi:10.1089/ten.2006.12.ft-104

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…It has become increasingly evident that common antibiotics alter cell behavior. Cohen et al (56) observed reduced proliferation and differentiation of murine embryonic stem cells (ESCs) while in the presence of P/S (56, 57). It has also been demonstrated that streptomycin and related aminoglycoside antibiotics behave as open‐channel blockers of mechanosensitive cation channels of undefined molecular origin in neurons, cardiac myocytes or skeletal muscle (31, 32, 58–60).…”

Section: Discussionmentioning

confidence: 99%

TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin

et al. 2017

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Transient receptor potential (TRP) channels are polymodal cell sensors responding to diverse stimuli and widely implicated in the developmental programs of numerous tissues. The evidence for an involvement of TRP family members in adipogenesis, however, is scant. We present the first comprehensive expression profile of all known 27 human TRP genes in mesenchymal progenitors cells during white or brown adipogenesis. Using positive trilineage differentiation as an exclusion criterion, TRP polycystic (P)3, and TPR melastatin (M)8 were found to be uniquely adipospecific. Knockdown of TRPP3 repressed the expression of the brown fat signature genes uncoupling protein (UCP)-1 and peroxisome proliferator-activated receptor γ coactivator (PGC)-1α as well as attenuated forskolin-stimulated uncoupled respiration. However, indices of generalized adipogenesis, such as lipid droplet morphology and fatty acid binding protein (FAPB)-4 expression, were not affected, indicating a principal mitochondrial role of TRPP3. Conversely, activating TRPM8 with menthol up-regulated UCP-1 expression and augmented uncoupled respiration predominantly in white adipocytes (browning), whereas streptomycin antagonized TRPM8-mediated calcium entry, downregulated UCP-1 expression, and mitigated uncoupled respiration; menthol was less capable of augmenting uncoupled respiration (thermogenesis) in brown adipocytes. TRPP3 and TRPM8 hence appear to be involved in the priming of mitochondria to perform uncoupled respiration downstream of adenylate cyclase. Our results also underscore the developmental caveats of using antibiotics in adipogenic studies.-Goralczyk, A., van Vijven, M., Koch, M., Badowski, C., Yassin, M. S., Toh, S.-A., Shabbir, A., Franco-Obregón, A., Raghunath, M. TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin.

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Section: Discussionmentioning

confidence: 99%

TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin

et al. 2017

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“…The routine use of antibiotics in culture media is not recommended, because they may select for antibiotic-resistant strains of microorganisms [68]. Cohen et al had suggested that the routine use of antibiotics in embryonic stem cell (ESC) cultures be avoided, as it might reduce the efficiency of the culture system [69]. Therefore, the use of antibiotics in glioma stem cell culture medium is not suggested.…”

Section: The Use Of Antibioticsmentioning

confidence: 99%

The necessity for standardization of glioma stem cell culture: a systematic review

Zhang

Yuan

et al. 2020

Stem Cell Res Ther

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Background: The cancer stem cell hypothesis is an old idea which has been revived in recent years for many cancers, including gliomas. However, this concept has become controversial due to a series of studies with conflicting results. Methods: A systematic literature search was conducted in PubMed and the Web of Science database to analyze studies using serum-free medium and its components in glioma stem cells, glioma stem-like cells, glioma-initiating cells, or glioma neurosphere cultures. All the studies reviewed were published between 1970 and 2019. We found that no standardized culture method was used, and the data were incomparable due to differing culture conditions and the use of media with different components. Conclusions: Here, we review the most commonly used serum-free media and added components for glioma stem cell culture while highlighting the function of each component used in the media. We emphasize the necessity for standardization of glioma stem cell culture and propose a standard culture medium to prevent bias in glioma stem cell research.

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“…The recently discovered Y‐27632 Rho‐associated kinase inhibitor appears to increase the survival rate of single cell‐dissociated hES cells to ∼27% ( Watanabe et al ., 2007 ). Also it has been reported that ES cells exhibit decreased growth rate and reduced differentiation efficiency when cultured with antibiotics such as gentamicin and combined penicillin–streptomycin ( Cohen et al ., 2006 ). Although the latter study was on mES cells, it provides further evidence that culture conditions of hES cells are still partly unexplored.…”

Section: Hes Cell Culturementioning

confidence: 99%

Human embryonic stem cells and lung regeneration

Varanou

Page

Minger

2008

British J Pharmacology

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Human embryonic stem cells are pluripotent cells derived from the inner cell mass of preimplantation stage embryos. Their unique potential to give rise to all differentiated cell types has generated great interest in stem cell research and the potential that it may have in developmental biology, medicine and pharmacology. The main focus of stem cell research has been on cell therapy for pathological conditions with no current methods of treatment, such as neurodegenerative diseases, cardiac pathology, retinal dysfunction and lung and liver disease. The overall aim is to develop methods of application either of pure cell populations or of whole tissue parts to the diseased organ under investigation. In the field of pulmonary research, studies using human embryonic stem cells have succeeded in generating enriched cultures of type II pneumocytes in vitro. On account of their potential of indefinite proliferation in vitro, embryonic stem cells could be a source of an unlimited supply of cells available for transplantation and for use in gene therapy. Uncovering the ability to generate such cell types will expand our understanding of biological processes to such a degree that disease understanding and management could change dramatically.

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Antibiotics Reduce the Growth Rate and Differentiation of Embryonic Stem Cell Cultures

Cited by 3 publications

References 27 publications

TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin

TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin

The necessity for standardization of glioma stem cell culture: a systematic review

Human embryonic stem cells and lung regeneration

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