Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone

Abstract

Abstract. Recombinant follicle stimulating hormone(rFSH), which play an important role in assisted reproductive technologies. However, the availability of a suitable system for the screening high production cell lines from transfected mammal cells for the production of rFSH is limited. The purpose of this study was to establish a method for screening cloned cell lines with high productivity from transfected HEK293 cells. Here, we adopt the antibiotics in combination with ELISA as a hyphenated technique to obtain production cell lines, and the results indicated that this a simple and efficient method in the cloned cell screening application.