Antibiotic treatment failure of uncomplicated urinary tract infections in primary care

Koh, Sky Wei Chee; Ng, Tony W.; Loh, Victor Weng Keong; Goh, Joyce; Low, Si Hui; Tan, Wei Zhi; Wong, Hung Chew; Durai, Pradeep; Sun, Louisa; Young, Doris; Tambyah, Paul Anantharajah

doi:10.1186/s13756-023-01282-4

“…Amoxicillin (AMOX) is an organic compound used as an antibiotic in the treatment of various infections, such as urinary tract [1], dental [2], chest [3], sinus [4], etc. This organic compound is found in drugs commercialized under different names, including (i) Amoksiklav (AMOX-drug), Augmentin, Amoclan, when AMOX is mixed with clavulanate potassium; (ii) Voquezna Triple Pak, which contains a mixture of AMOX, clarithromycin and vonoprazan; (iii) Omeclamox-Pak, when active compounds consist of AMOX, clarithromycin and omeprazole; and (iv) Prevpac, when the ingredients include AMOX, clarithromycin and lansoprazole.…”

Section: Introductionmentioning

confidence: 99%

Nanohybrid Composites Based on TiO2 and Single-Walled Carbon Nanohorns as Promising Catalysts for Photodegradation of Amoxicillin

Cercel,

Androne,

Florica

et al. 2023

Molecules

2

0

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In this work, applications of nanohybrid composites based on titanium dioxide (TiO2) with anatase crystallin phase and single-walled carbon nanohorns (SWCNHs) as promising catalysts for the photodegradation of amoxicillin (AMOX) are reported. In this order, TiO2/SWCNH composites were prepared by the solid-state interaction of the two chemical compounds. The increase in the SWCNH concentration in the TiO2/SWCNH composite mass, from 1 wt.% to 5 wt.% and 10 wt.% induces (i) a change in the relative intensity ratio of the Raman lines located at 145 and 1595 cm−1, which are attributed to the Eg(1) vibrational mode of TiO2 and the graphitic structure of SWCNHs; and (ii) a gradual increase in the IR band absorbance at 1735 cm−1 because of the formation of new carboxylic groups on the SWCNHs’ surface. The best photocatalytic properties were obtained for the TiO2/SWCNH composite with a SWCNH concentration of 5 wt.%, when approx. 92.4% of AMOX removal was achieved after 90 min of UV irradiation. The TiO2/SWCNH composite is a more efficient catalyst in AMOX photodegradation than TiO2 as a consequence of the SWCNHs’ presence, which acts as a capture agent for the photogenerated electrons of TiO2 hindering the electron–hole recombination. The high stability of the TiO2/SWCNH composite with a SWCNH concentration of 5 wt.% is proved by the reusing of the catalyst in six photodegradation cycles of the 98.5 μM AMOX solution, when the efficiency decreases from 92.4% up to 78%.

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Enzymes in Synergy: Bacteria Specific Molecular Probe for Locoregional Imaging of Urinary Tract Infection in vivo

Xin,

Kwek,

An

et al. 2024

Angewandte Chemie

0

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Uropathogenic Escherichia coli (UPECs) is a leading cause for urinary tract infections (UTI), accounting for 70‐90% of community or hospital‐acquired bacterial infections owing to high recurrence, imprecision in diagnosis and management, and increasing prevalence of antibiotic resistance. Current methods for clinical UPECs detection still rely on labor‐intensive urine cultures that impede rapid and accurate diagnosis for timely UTI therapeutic management. Herein, we developed a first‐in‐class near‐infrared (NIR) UPECs fluorescent probe (NO‐AH) capable of specifically targeting UPECs through its collaborative response to bacterial enzymes, enabling locoregional imaging of UTIs both in vitro and in vivo. Our NO‐AH probe incorporates a dual protease activatable moiety, which first reacts with OmpT, an endopeptidase abundantly present on outer membrane of UPECs, releasing an intermediate amino acid residue conjugated with a NIR hemicyanine fluorophore. Such liberated fragment would be subsequently recognized by aminopeptidase (APN) within periplasm of UPECs, activating localized fluorescence for precise imaging of UTIs in complex living environments. The peculiar specificity and selectivity of NO‐AH, facilitated by the collaborative action of bacterial enzymes, features a timely and accurate identification of UPECs‐infected UTIs, which could overcome misdiagnosis in conventional urine tests, thus opening new avenues towards reliable UTI diagnosis and personalized antimicrobial therapy management.

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Enzymes in Synergy: Bacteria Specific Molecular Probe for Locoregional Imaging of Urinary Tract Infection in vivo

Xin,

Kwek,

An

et al. 2024

Angew Chem Int Ed

0

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Uropathogenic Escherichia coli (UPECs) is a leading cause for urinary tract infections (UTI), accounting for 70‐90% of community or hospital‐acquired bacterial infections owing to high recurrence, imprecision in diagnosis and management, and increasing prevalence of antibiotic resistance. Current methods for clinical UPECs detection still rely on labor‐intensive urine cultures that impede rapid and accurate diagnosis for timely UTI therapeutic management. Herein, we developed a first‐in‐class near‐infrared (NIR) UPECs fluorescent probe (NO‐AH) capable of specifically targeting UPECs through its collaborative response to bacterial enzymes, enabling locoregional imaging of UTIs both in vitro and in vivo. Our NO‐AH probe incorporates a dual protease activatable moiety, which first reacts with OmpT, an endopeptidase abundantly present on outer membrane of UPECs, releasing an intermediate amino acid residue conjugated with a NIR hemicyanine fluorophore. Such liberated fragment would be subsequently recognized by aminopeptidase (APN) within periplasm of UPECs, activating localized fluorescence for precise imaging of UTIs in complex living environments. The peculiar specificity and selectivity of NO‐AH, facilitated by the collaborative action of bacterial enzymes, features a timely and accurate identification of UPECs‐infected UTIs, which could overcome misdiagnosis in conventional urine tests, thus opening new avenues towards reliable UTI diagnosis and personalized antimicrobial therapy management.

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Antibiotic treatment failure of uncomplicated urinary tract infections in primary care

Cited by 4 publications

References 18 publications

Nanohybrid Composites Based on TiO2 and Single-Walled Carbon Nanohorns as Promising Catalysts for Photodegradation of Amoxicillin

Nanohybrid Composites Based on TiO2 and Single-Walled Carbon Nanohorns as Promising Catalysts for Photodegradation of Amoxicillin

Enzymes in Synergy: Bacteria Specific Molecular Probe for Locoregional Imaging of Urinary Tract Infection in vivo

Enzymes in Synergy: Bacteria Specific Molecular Probe for Locoregional Imaging of Urinary Tract Infection in vivo

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