Antibiotic Susceptibility Testing by a Standardized Single Disk Method

Abstract

in Seattle, on a standardized single-disk method for antibiotic susceptibility testing ".. . consolidate(s) and update(s) previous descriptions of the method and provide(s) a concise outline for its performance and interpretation." Clinical microbiologists were relieved that finally a disk diffusion method had been standardized, could be used with ease, and provided reliable results as compared with minimum inhibitory concentration tests. The pivotal role of Hans Ericsson's theoretical and practical studies (H. Ericsson and G. Svartz-Malmberg, Antibiot. Chemother. 6:41-74, 1959), as well as earlier reports by some of the authors of the publications cited, must be mentioned as a matter of fairness. Most of the recommendations given are still valid today even though some of the antimicrobial agents are obsolete, new ones have been added, some zone sizes had to be modified, and new media were designed for Haemophilus influenzae and Neisseria gonorrhoeae. Recommendations of the National Committee for Clinical Laboratory Standards continue to be based on this publication; the "Kirby-Bauer" method is, among the many disk methods used in other countries, still the one that has been researched most thoroughly and updated continuously.

Phytochemical and antimicrobial evaluation of selected medicinal plants in Meru community of Kenya

“…Filter paper disc-agar diffusion procedure, known as the Kirby-Bauer method (Bauer et al 1966), was used to determine the drug susceptibility of Pseudomonas aeruginosa, E. coli, Bacillus subtilis, Staphylococcus aureus and C. albicans. These microorganisms were obtained from the Department of Botany.…”

In Vitro Antibacterial Effects of Chloroform, Methanol and Water Extracts of Croton Macrostachyus Stem Bark Against Escherichia Coli and Staphylococcus Aureus Standard and Clinical Strains

“…The MICs of plant extracts was determined using a broth dilution method as described by Bauer et al (1966).…”

A Comparative Phytochemical and Biological Study between Different Solvent Extracts of <i>Bombax ceiba </i>Roots Available in Bangladesh

“…Five Gram-positive and six Gram-negative bacterial strains were used in the antibacterial assay following the disc diffusion method [20]. Suspension of each microorganism (100 µl, containing approximately 100 -150 CFU/ml) was spread over nutrient agar media and dried, sterile filter paper discs of 6 mm diameter were placed gently on those agar plates after impregnated with 300 µg and 600 µg of different extracts prepared by dissolving them in methanol followed by evaporation.…”