Antibiotic susceptibility profiles of Mycoplasma synoviae strains originating from Central and Eastern Europe

Abstract

Background Mycoplasma synoviae causes infectious synovitis and respiratory diseases in chickens and turkeys and may lead to egg shell apex abnormalities in chickens; hence possesses high economic impact on the poultry industry. Control of the disease consists of eradication, vaccination or medication. The aim of the present study was to determine the in vitro susceptibility to 14 different antibiotics and an antibiotic combination of M. synoviae strains originating from Hungary and other countries of Central and Eastern Europe.ResultsMinimal inhibitory concentration (MIC) values of a total of 41 M. synoviae strains were determined by the microbroth dilution method. The strains were collected between 2002 and 2016 and originated from Hungary (n = 26), Austria (n = 3), the Czech Republic (n = 3), Slovenia (n = 3), Ukraine (n = 3), Russia (n = 2) and Serbia (n = 1). Tetracyclines (with MIC50 values of 0.078 μg/ml, ≤0.25 μg/ml and 0.5 μg/ml for doxycycline, oxytetracycline and chlortetracycline, respectively), macrolides (with MIC50 values of ≤0.25 μg/ml for tylvalosin, tylosin and tilmicosin), pleuromutilins (with MIC50 values of 0.078 μg/ml and ≤0.039 μg/ml for tiamulin and valnemulin) and the combination of lincomycin and spectinomycin (MIC50 1 μg/ml (0.333/0.667 μg/ml)) were found to be the most effective antibiotic agents against M. synoviae in vitro. High MIC values were detected in numerous strains for fluoroquinolones (with MIC50 values of 1.25 μg/ml and 2.5 μg/ml for enrofloxacin and difloxacin), neomycin (MIC50 32 μg/ml), spectinomycin (MIC50 2 μg/ml), lincomycin (MIC50 0.5 μg/ml) and florfenicol (MIC50 4 μg/ml). Nevertheless, strains with elevated MIC values were detected for most of the applied antibiotics.ConclusionsIn the medical control of M. synoviae infections the preliminary in vitro antibiotic susceptibility testing and the careful evaluation of the data are crucial. Based on the in vitro examinations doxycycline, oxytetracycline, tylvalosin, tylosin and pleuromutilins could be recommended for the therapy of M. synoviae infections in the region.Electronic supplementary materialThe online version of this article (10.1186/s12917-017-1266-2) contains supplementary material, which is available to authorized users.

In vitroEvaluation of Various Antimicrobials against FieldMycoplasma gallisepticumandMycoplasma synoviaeIsolates in Egypt

“…Among macrolides group, tylvalosin was the most effective drug against both MG and MS isolates with an MIC range of 0.015-0.030 µg/mL. This finding is in a complete agreement with other research results in Iran [30], UK [5] and Europe [15]. This could be attributed to the recent introduction of tylvalosin as a newer antibiotic against MG and MS isolates in Egypt.…”

“…Interpretation of antimicrobial susceptibility results is hampered by lacking of the official breakpoints. In these cases, interpreting the data of our study is based on values previously used in other publications [8,15].…”

Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and Mycoplasma synoviae

“…MS is a major bacterial pathogen affecting the poultry industry worldwide with infections incurring enormous economic losses (Kreizinger et al, 2017;Sun et al, 2017;Xue et al, 2017;Kursa et al, 2019). Clinical signs of MS infection include infectious synovitis, primarily of the hock joints and foot pads (Kleven et al, 1975).…”

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