Surgical site infection are the most common nosocomial infection accounting for 14% healthcare associated infection and are estimated to double the post-operative stay and significantly increase the cost of care. Surgical site infection has been considered as the third regularly occurring infection according to national nosocomial infection surveillance system.To determine the bacterial agents causing surgical site infection and antimicrobial susceptibility pattern of isolated microorganisms.: Isolate and identify pathogens of surgical site infection. To determine antimicrobial resistance and sensitivity pattern of isolated wound microbes. To determine the frequency of pathogens of surgical site infection.: Samples were cultured on Blood agar and MacConkey agar then incubated at 37 C for 24 hours. Any growth for further confirmed by Gram stain and appropriate biochemical tests, and then Antibiotic sensitivity test was done.: In this study 106 (61.7) were of male and 66 (38.3) were of female total 172 were examined. In our study total 172 patients included in which 142 pathogenic organism were isolated. The most common pathogenic organism found to be 44 isolates (30.99%) followed by 41 isolates (28.87%) 23 isolates (16.20%), 18 isolates (12.68%), 9 isolates (6.34%), 5 isolates (3.52%), 1 isolate (0.70%) and 1 isolate (0.70%). Antimicrobial resistance always pose challenges for clinician for treating wound infection the present study guide clinician about common pathogens and countered in pus sample furthermore it help the clinician to select and treat patients with proper antibiotics and decreased mortality and morbidity.