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In this study, 180 isolates of Pseudomonas aeruginosa were isolated from patients suffering from various infections, including urinary tract infections, burns, ear infections, tonsillitis, and pneumonia. Specimens were taken from patients hospitalized in Al-Hakim General Hospital, Al-Sadr Medical City, and the Burn Center in Najaf Al-Ashraf, and they were transferred to the Microbiology Laboratory in the Biology Department in the College of Science. P. aeruginosa have been isolated from Specimens taken from patients with these various infections. Isolation methods were different, including culture on MacConkey medium, blood agar, as well as various biochemical and Vitek tests. Susceptibility testing was also performed on these bacteria for six families according to CLSI. These families are penicillins, aminoglycosides, carbopenems, cephalosporins, fluoroquinins, and lipopeptides. It was noted that forty isolates were multi-resistant to these antibiotics. It was also revealed that resistant isolates formed biofilms using a flat microliter. The effectiveness of domestic and imported magnesium oxide nanoparticles on resistant isolates was conducted. These two types were taken at concentrations of 100, 150, and 200 μg/ml. Imported MgO nanoparticles were more effective than domestic ones. It was also observed that the effect of magnesium oxide nanoparticles on resistant bacteria increased with increasing concentration. Antibiotics resistant to Pseudomonas aeruginosa bacteria were also taken and mixed with imported and local magnesium oxide nanoparticles with an optimal concentration of (200) μg/ml. The effect of magnesium oxide nanoparticles combined with antibiotics was greater than if the nanoparticles were alone.
In this study, 180 isolates of Pseudomonas aeruginosa were isolated from patients suffering from various infections, including urinary tract infections, burns, ear infections, tonsillitis, and pneumonia. Specimens were taken from patients hospitalized in Al-Hakim General Hospital, Al-Sadr Medical City, and the Burn Center in Najaf Al-Ashraf, and they were transferred to the Microbiology Laboratory in the Biology Department in the College of Science. P. aeruginosa have been isolated from Specimens taken from patients with these various infections. Isolation methods were different, including culture on MacConkey medium, blood agar, as well as various biochemical and Vitek tests. Susceptibility testing was also performed on these bacteria for six families according to CLSI. These families are penicillins, aminoglycosides, carbopenems, cephalosporins, fluoroquinins, and lipopeptides. It was noted that forty isolates were multi-resistant to these antibiotics. It was also revealed that resistant isolates formed biofilms using a flat microliter. The effectiveness of domestic and imported magnesium oxide nanoparticles on resistant isolates was conducted. These two types were taken at concentrations of 100, 150, and 200 μg/ml. Imported MgO nanoparticles were more effective than domestic ones. It was also observed that the effect of magnesium oxide nanoparticles on resistant bacteria increased with increasing concentration. Antibiotics resistant to Pseudomonas aeruginosa bacteria were also taken and mixed with imported and local magnesium oxide nanoparticles with an optimal concentration of (200) μg/ml. The effect of magnesium oxide nanoparticles combined with antibiotics was greater than if the nanoparticles were alone.
A total of 74 clinical samples Formalin-Fixed Paraffin-Embedded (FFPE) were collected from patients diagnosed with prostate cancer (PCa) aged between 41 and 90 years and these samples were obtained from patients treated at notable medical institutions like Al-Sadr Medical City and leading clinical laboratories in Al-Najaf City, Iraq, during the period of January to December 2023. The current study indicated the potential role of the JCV virus in provoking prostatitis, which may lead to the emergence and development of prostate cancer in males compared to males who do not suffer from viral infection. The present study showed the presence of JCV virus DNA, as the percentage of positive samples reached (11, 14.864%) compared to negative samples (63, 85.135%). The current study showed a significant increase in the level of ICAM-2 biomarker in patients with JCV-positive prostate cancer, reaching (818.500±42.748 pg/ml) compared with patients with JCV-negative, reaching (502.925±58.037 pg/ml). Neprilysin (NEP) levels in the current study were significantly high in for JCV-positive patients and those with prostate cancer, reaching (4.275±0.376 pg/ml) compared with JCV-negative patients, reaching (3.131±0.232 pg/ml).
The current study was done to isolation and indentification of the Staphlococcus aureus isolates associated with polycystic ovarian syndrome patients and determine the immunological status for these patients. Inflammatory markers have been linked to polycystic ovary syndrome (PCOS). Because ovulation is a quasi-inflammatory process, endothelial adipose tissue can stimulate an inflammatory response and maintain inflammation in adipocytes. By influencing the release of inflammatory cytokines from this downregulated inflammation, polycystic ovary syndrome can arise. The study included 105 clinical specimens, that include age (20-45) years which were collected from November 2022 to June 2023. The specimens included blood specimens and vaginal swabs. They visit Imam Al-Sadiq Hospital and Babylon Teaching Hospital for Maternity and Children in Babylon Governorate. The results of vaginal swabs culture, biochemical tests and vitic2 for isolated bacteria from patients were revealed that Gram-positive bacteria isolates were dominant, amounting to 51 (54.83%), while the percentage of Gram-negative bacteria was 37 (39.78%), while fungi percentage was 5 (5.37%). The results showed that Staphylococcus aureus is the most common bacteria among Gram-positive bacteria, as it constituted 38 (40.86%), followed by Lactobacillus spp. As it accounted for 11 (11.82%) of Staphylococcus epidermidis by 2 (2.15%). The Gram-negative isolates included Escherichiae coli, Klebsiella pneumoniae, and Pseudomonase aerogenosa 23 (24.73%), 9(9.67%) and 5 (5.37%), respectively also the result demonstrates that IL-10, IL-13 level significantly elevation in PCOS, PCOD patients group.
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